Whole gene analysis of a genotype G29P[6] human rotavirus strain identified in Central African Republic

Abstract Objective Rotavirus A (RVA) remains the main causative agent of gastroenteritis in young children and the young of many mammalian and avian species. In this study we describe a RVA strain detected from a 6-month-old child from Central African Republic (CAR). Results We report the 11 open reading frame sequences of a G29-P[6]-I2-R2-C2-M2-A2-N2-T2-E2-H2 rotavirus strain, RVA/Human-wt/CAR/CAR91/2014/G29P[6]. Nine genes (VP1–VP3, VP6, NSP1–NSP5) shared 90–100% sequence similarities with genogroup 2 rotaviruses. Phylogenetically, backbone genes, except for VP3 and NSP4 genes, were linked with cognate gene sequences of human DS-1-like genogroup 2, hence their genetic origin. The VP3 and NSP4 genes, clustered genetically with both human and animal strains, an indication genetic reassortment human and animal RVA strains has taken place. The VP7 gene shared nucleotide (93–94%) and amino acid (95.5–96.7%) identities with Kenyan and Belgian human G29 strains, as well as to buffalo G29 strain from South Africa, while the VP4 gene most closely resembled P[6]-lineage I strains from Africa and Bangladesh (97%).

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Ilarviruses Encode a Cucumovirus-Like 2b Gene That Is Absent in Other Genera within the Bromoviridae

Journal of Virology ◽

10.1128/jvi.72.8.6956-6959.1998 ◽

1998 ◽

Vol 72 (8) ◽

pp. 6956-6959 ◽

Cited By ~ 33

Author(s):

Hong-Wu Xin ◽

Liang-Hui Ji ◽

Simon W. Scott ◽

Robert H. Symons ◽

Shou-Wei Ding

Keyword(s):

Open Reading Frame ◽

Latent Virus ◽

Evolutionary Origin ◽

Protein Species ◽

Subgenomic Rna ◽

Reading Frame ◽

2B Protein ◽

Sequence Similarities

ABSTRACT We found that RNA 2 of the four ilarviruses sequenced to date encodes an additional conserved open reading frame (ORF), 2b, that overlaps the 3′ end of the previously known ORF, 2a. A novel RNA species of 851 nucleotides was found to accumulate to high levels in plants infected with spinach latent virus (SpLV). Further analysis showed that RNA 4A is a subgenomic RNA of RNA 2 and encodes all of ORF 2b. Moreover, a protein species of the size expected for SpLV ORF 2b was translated in vitro from the RNA 4A-containing virion RNAs. The data support the suggestion that the SpLV 2b protein is translated in vivo. The 2b gene of ilarviruses, which is not encoded by alfamoviruses and bromoviruses, shares several features with the previously reported cucumovirus 2b gene; however, their encoded proteins share no detectable sequence similarities. The evolutionary origin of the 2b gene is discussed.

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YojI of Escherichia coli Functions as a Microcin J25 Efflux Pump

Journal of Bacteriology ◽

10.1128/jb.187.10.3465-3470.2005 ◽

2005 ◽

Vol 187 (10) ◽

pp. 3465-3470 ◽

Cited By ~ 28

Author(s):

Mónica A. Delgado ◽

Paula A. Vincent ◽

Ricardo N. Farías ◽

Raúl A. Salomón

Keyword(s):

Escherichia Coli ◽

Efflux Pump ◽

Open Reading Frame ◽

Peptide Antibiotic ◽

Toxic Level ◽

Reading Frame ◽

Increased Sensitivity ◽

Multicopy Vector ◽

Microcin J25 ◽

Sequence Similarities

ABSTRACT In the present study, we showed that yojI, an Escherichia coli open reading frame with an unknown function, mediates resistance to the peptide antibiotic microcin J25 when it is expressed from a multicopy vector. Disruption of the single chromosomal copy of yojI increased sensitivity of cells to microcin J25. The YojI protein was previously assumed to be an ATP-binding-cassette-type exporter on the basis of sequence similarities. We demonstrate that YojI is capable of pumping out microcin molecules. Thus, one obvious explanation for the protective effect against microcin J25 is that YojI action keeps the intracellular concentration of the peptide below a toxic level. The outer membrane protein TolC in addition to YojI is required for export of microcin J25 out of the cell. Microcin J25 is thus the first known substrate for YojI.

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