scholarly journals Phenotypical and functional characteristics of mesenchymal stem cells from bone marrow: comparison of culture using different media supplemented with human platelet lysate or fetal bovine serum

2012 ◽  
Vol 3 (1) ◽  
pp. 6 ◽  
Author(s):  
Nesrine Azouna ◽  
Faouzi Jenhani ◽  
Zohra Regaya ◽  
Lamia Berraeis ◽  
Tarek Othman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Functional Characteristics ◽  
Human Platelet Lysate ◽  
Fetal Bovine

scholarly journals Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
M. Guiotto ◽  
W. Raffoul ◽  
A. M. Hart ◽  
M. O. Riehle ◽  
P. G. di Summa
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Search Algorithm ◽  
Platelet Lysate ◽  
Pubmed Database ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Abstract Background Foetal bovine serum (FBS), is the most commonly used culture medium additive for in vitro cultures, despite its undefined composition, its potential immunogenicity and possible prion/zoonotic transmission. For these reasons, significant efforts have been targeted at finding a substitute, such as serum free-media or human platelet-lysates (hPL). Our aim is to critically appraise the state-of-art for hPL in the published literature, comparing its impact with FBS. Materials and methods In June 2019 a systematic search of the entire Web of Science, Medline and PubMed database was performed with the following search terms: (mesenchymal stem cells) AND (fetal bovine serum OR fetal bovine calf) AND (human platelet lysate). Excluded from this search were review articles that were published before 2005, manuscripts in which mesenchymal stem cells (MSCs) were not from human sources, and when the FBS controls were missing. Results Based on our search algorithm, 56 papers were selected. A review of these papers indicated that hMSCs cultured with hPL showed a spindle-shaped elongated morphology, had higher proliferation indexes, similar cluster of differentiation (CD) markers and no significant variation in differentiation lineage (osteocyte, adipocyte, and chondrocyte) compared to those cultured with FBS. Main sources of primary hMSCs were either fat tissue or bone marrow; in a few studies cells isolated from alternative sources showed no relevant difference in their response. Conclusion Despite the difference in medium choice and a lack of standardization of hPL manufacturing, the majority of publications support that hPL was at least as effective as FBS in promoting adhesion, survival and proliferation of hMSCs. We conclude that hPL should be considered a viable alternative to FBS in hMSCs culture—especially with a view for their clinical use.

scholarly journals Human platelet lysate as an alternative to fetal bovine serum for culture and endothelial differentiation of human amniotic fluid mesenchymal stem cells

2019 ◽  
Author(s):  
Waleephan Tancharoen ◽  
Sirinda Aungsuchawan ◽  
Peraphan Pothacharoen ◽  
Kanokkan Bumroongkit ◽  
Chaniporn Puaninta ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Amniotic Fluid ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Endothelial Differentiation ◽  
Human Amniotic Fluid ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Comparison of fetal bovine serum and human platelet lysate in cultivation and differentiation of dental pulp stem cells into hepatic lineage cells

2014 ◽  
Vol 88 ◽  
pp. 142-153 ◽  
Author(s):  
Punitha Vasanthan ◽  
Nareshwaran Gnanasegaran ◽  
Vijayendran Govindasamy ◽  
Aimi Naim Abdullah ◽  
Pukana Jayaraman ◽  
...  
Keyword(s):  
Stem Cells ◽  
Dental Pulp ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Dental Pulp Stem Cells ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine

scholarly journals Human Platelet Lysate is a Good Alternative to Fetal Bovine Serum in Bone Marrow Karyotyping Medium

2020 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Abdulkader Mima ◽  
Abduljalil Ghriwati ◽  
Khaled Khanje
Keyword(s):  
Bone Marrow ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Good Alternative ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Efficient replacing of fetal bovine serum with human platelet releasate during propagation and differentiation of human bone marrow-derived mesenchymal stem cells to functional hepatocytes-like cells

Vox Sanguinis ◽  
2008 ◽  
Vol 95 (2) ◽  
pp. 149-158 ◽  
Author(s):  
S. Kazemnejad ◽  
A. Allameh ◽  
A. Gharehbaghian ◽  
M. Soleimani ◽  
N. Amirizadeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Platelet Releasate ◽  
Fetal Bovine

Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells

2013 ◽  
Vol 95 (2) ◽  
pp. 693-698 ◽  
Author(s):  
Jong-pil Seo ◽  
Nao Tsuzuki ◽  
Shingo Haneda ◽  
Kazutaka Yamada ◽  
Hidefumi Furuoka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
In Vitro Expansion ◽  
Fetal Bovine ◽  
Equine Bone

scholarly journals HUMAN PLATELET LYSATE IS A GOOD ALTERNATIVE TO FETAL BOVINE SERUM IN BONE MARROW KARYOTYPING MEDIUM

2021 ◽  
Vol 44 (1) ◽  
pp. 111-118
Author(s):  
Abdulkader Mima ◽  
Abdel Galil Ghriwati ◽  
Khalid Khanji
Keyword(s):  
Bone Marrow ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Good Alternative ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Human platelet lysate is an alternative to fetal bovine serum for large-scale expansion of bone marrow-derived mesenchymal stromal cells

2012 ◽  
Vol 34 (7) ◽  
pp. 1367-1374 ◽  
Author(s):  
Sanjay Gottipamula ◽  
Archana Sharma ◽  
Sagar Krishnamurthy ◽  
Anish Sen Majumdar ◽  
Raviraja N. Seetharam
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Bovine Serum ◽  
Large Scale ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Human Platelet Lysate ◽  
Fetal Bovine
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