Comparison of allogeneic platelet lysate and fetal bovine serum for in vitro expansion of equine bone marrow-derived mesenchymal stem cells

2013 ◽  
Vol 95 (2) ◽  
pp. 693-698 ◽  
Author(s):  
Jong-pil Seo ◽  
Nao Tsuzuki ◽  
Shingo Haneda ◽  
Kazutaka Yamada ◽  
Hidefumi Furuoka ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
In Vitro Expansion ◽  
Fetal Bovine ◽  
Equine Bone

Comparative epigenetic influence of autologous versus fetal bovine serum on mesenchymal stem cells through in vitro osteogenic and adipogenic differentiation

2016 ◽  
Vol 344 (2) ◽  
pp. 176-182 ◽  
Author(s):  
Nesa Fani ◽  
Reihane Ziadlou ◽  
Maryam Shahhoseini ◽  
Mohamadreza Baghaban Eslaminejad
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Adipogenic Differentiation ◽  
Fetal Bovine

Human Serum is as Efficient as Fetal Bovine Serum in Supporting Proliferation and Differentiation of Human Multipotent Stromal (Mesenchymal) Stem Cells In Vitro and In Vivo

2011 ◽  
Vol 7 (4) ◽  
pp. 860-868 ◽  
Author(s):  
Abdullah Aldahmash ◽  
Mandana Haack-Sørensen ◽  
May Al-Nbaheen ◽  
Linda Harkness ◽  
Basem M. Abdallah ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Human Serum ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Proliferation And Differentiation ◽  
Fetal Bovine

scholarly journals Genetic and epigenetic instability of human bone marrow mesenchymal stem cells expanded in autologous serum or fetal bovine serum

2008 ◽  
Vol 52 (8) ◽  
pp. 1033-1042 ◽  
Author(s):  
John-Arne Dahl ◽  
Shivali Duggal ◽  
Neralie Coulston ◽  
Douglas Millar ◽  
John Melki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Human Bone ◽  
Autologous Serum ◽  
Epigenetic Instability ◽  
Marrow Mesenchymal Stem Cells ◽  
Fetal Bovine

scholarly journals Human platelet lysate to substitute fetal bovine serum in hMSC expansion for translational applications: a systematic review

2020 ◽  
Vol 18 (1) ◽  
Author(s):  
M. Guiotto ◽  
W. Raffoul ◽  
A. M. Hart ◽  
M. O. Riehle ◽  
P. G. di Summa
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Search Algorithm ◽  
Platelet Lysate ◽  
Pubmed Database ◽  
Human Platelet Lysate ◽  
Fetal Bovine

Abstract Background Foetal bovine serum (FBS), is the most commonly used culture medium additive for in vitro cultures, despite its undefined composition, its potential immunogenicity and possible prion/zoonotic transmission. For these reasons, significant efforts have been targeted at finding a substitute, such as serum free-media or human platelet-lysates (hPL). Our aim is to critically appraise the state-of-art for hPL in the published literature, comparing its impact with FBS. Materials and methods In June 2019 a systematic search of the entire Web of Science, Medline and PubMed database was performed with the following search terms: (mesenchymal stem cells) AND (fetal bovine serum OR fetal bovine calf) AND (human platelet lysate). Excluded from this search were review articles that were published before 2005, manuscripts in which mesenchymal stem cells (MSCs) were not from human sources, and when the FBS controls were missing. Results Based on our search algorithm, 56 papers were selected. A review of these papers indicated that hMSCs cultured with hPL showed a spindle-shaped elongated morphology, had higher proliferation indexes, similar cluster of differentiation (CD) markers and no significant variation in differentiation lineage (osteocyte, adipocyte, and chondrocyte) compared to those cultured with FBS. Main sources of primary hMSCs were either fat tissue or bone marrow; in a few studies cells isolated from alternative sources showed no relevant difference in their response. Conclusion Despite the difference in medium choice and a lack of standardization of hPL manufacturing, the majority of publications support that hPL was at least as effective as FBS in promoting adhesion, survival and proliferation of hMSCs. We conclude that hPL should be considered a viable alternative to FBS in hMSCs culture—especially with a view for their clinical use.

Increased proliferation of human synovial mesenchymal stem cells with autologous human serum: Comparisons with bone marrow mesenchymal stem cells and with fetal bovine serum

2008 ◽  
Vol 58 (2) ◽  
pp. 501-510 ◽  
Author(s):  
Akimoto Nimura ◽  
Takeshi Muneta ◽  
Hideyuki Koga ◽  
Tomoyuki Mochizuki ◽  
Koji Suzuki ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Serum ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Marrow Mesenchymal Stem Cells ◽  
Fetal Bovine

Adult bovine platelet lysate-derived serum “NeoSERA” is safe, less ethical and powerful alternative to fetal bovine serum for the culture of mesenchymal stem cells

Cytotherapy ◽  
2019 ◽  
Vol 21 (5) ◽  
pp. S80-S81
Author(s):  
K. Yamahara ◽  
T. Sudo ◽  
A. Hamada ◽  
K. Yoshihara ◽  
S. Yoshihara ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Fetal Bovine Serum ◽  
Platelet Lysate ◽  
Fetal Bovine

Efficient replacing of fetal bovine serum with human platelet releasate during propagation and differentiation of human bone marrow-derived mesenchymal stem cells to functional hepatocytes-like cells

Vox Sanguinis ◽  
2008 ◽  
Vol 95 (2) ◽  
pp. 149-158 ◽  
Author(s):  
S. Kazemnejad ◽  
A. Allameh ◽  
A. Gharehbaghian ◽  
M. Soleimani ◽  
N. Amirizadeh ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Bovine Serum ◽  
Human Platelet ◽  
Fetal Bovine Serum ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Platelet Releasate ◽  
Fetal Bovine
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