Development and Application of a Real-Time Quantitative PCR for Prenatal Detection of Fetal  0-Thalassemia from Maternal Plasma

2006 ◽  
Vol 1075 (1) ◽  
pp. 103-107 ◽  
Author(s):  
W. TUNGWIWAT ◽  
S. FUCHAROEN ◽  
G. FUCHAROEN ◽  
T. RATANASIRI ◽  
K. SANCHAISURIYA
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Maternal Plasma ◽  
Real Time Quantitative Pcr ◽  
Prenatal Detection

scholarly journals Prenatal Detection by Real-Time Quantitative PCR and Characterization of a New CFTR Deletion, 3600+15kbdel5.3kb (or CFTRdele19)

2000 ◽  
Vol 46 (9) ◽  
pp. 1417-1420 ◽  
Author(s):  
Bruno Costes ◽  
Emmanuelle Girodon ◽  
Dominique Vidaud ◽  
Elisabeth Flori ◽  
Azarnouche Ardalan ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Real Time Quantitative Pcr ◽  
Prenatal Detection

Fetal RhD genotyping by real time quantitative PCR in maternal plasma of RhD-negative pregnant women from the Sahel of Tunisia

2012 ◽  
Vol 70 (6) ◽  
pp. 683-688 ◽  
Author(s):  
Hajer Moussa ◽  
Marthe Tsochandaridis ◽  
Saloua Jemni-Yacoub ◽  
Slama Hmida ◽  
Hédi Khairi ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Real Time ◽  
Quantitative Pcr ◽  
Maternal Plasma ◽  
Real Time Quantitative Pcr

Optimized Real-Time Quantitative PCR Measurement of Male Fetal DNA in Maternal Plasma

2006 ◽  
Vol 1075 (1) ◽  
pp. 347-349 ◽  
Author(s):  
B. G ZIMMERMANN ◽  
W. HOLZGREVE ◽  
N. AVENT ◽  
S. HAHN
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Maternal Plasma ◽  
Real Time Quantitative Pcr ◽  
Fetal Dna

scholarly journals Optimized Real-Time Quantitative PCR Measurement of Male Fetal DNA in Maternal Plasma

2005 ◽  
Vol 51 (9) ◽  
pp. 1598-1604 ◽  
Author(s):  
Bernhard Zimmermann ◽  
Ahmad El-Sheikhah ◽  
Kypros Nicolaides ◽  
Wolfgang Holzgreve ◽  
Sinuhe Hahn
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
Pcr Amplification ◽  
First Trimester ◽  
Single Copy ◽  
Maternal Plasma ◽  
Probit Regression ◽  
Real Time Quantitative Pcr ◽  
Fetal Dna ◽  
First Trimester Of Pregnancy

Abstract Background: Circulating fetal DNA (cfDNA) in maternal plasma has been measured to investigate its possible relationship with pregnancy-related disorders, including fetal trisomy 21 and preeclampsia. The circulating concentrations of single-copy fetal genes, however, are close to the detection limits of PCR methods. Methods: We optimized a protocol for the real-time quantitative PCR amplification of the multicopy sequence DYS14 on the Y-chromosome. This was compared with an established real-time PCR assay for the single-copy SRY gene. Results: By probit regression analysis, the measurements of male DNA by the DYS14 assay had a 10-fold lower detection limit (0.4 genome equivalents) than did measurements of SRY. For plasma samples from women in the first trimester of pregnancy, imprecision (CV) was 2%–22% when amplifying DYS14 compared with 26%–140% for SRY. Conclusions: The low copy numbers of fetal DNA in plasma of women in the first trimester of pregnancy cannot be measured precisely when targeting single-copy sequences. Better results are obtained by amplifying a sequence that is present in multiple copies per male genome.

scholarly journals Quantification and Stability of Human Adenoviruses and Polyomavirus JCPyV in Wastewater Matrices

2006 ◽  
Vol 72 (12) ◽  
pp. 7894-7896 ◽  
Author(s):  
Silvia Bofill-Mas ◽  
Nestor Albinana-Gimenez ◽  
Pilar Clemente-Casares ◽  
Ayalkibet Hundesa ◽  
Jesus Rodriguez-Manzano ◽  
...  
Keyword(s):  
Real Time ◽  
Quantitative Pcr ◽  
High Stability ◽  
Wastewater Sludge ◽  
Human Polyomavirus ◽  
Sewage Effluent ◽  
Viral Contamination ◽  
Human Adenoviruses ◽  
Urban Sewage ◽  
Real Time Quantitative Pcr

ABSTRACT Human adenoviruses (HAdV) and human polyomavirus JCPyV have been previously proposed as indicators of fecal viral contamination in the environment. Different wastewater matrices have been analyzed by applying real-time quantitative PCR procedures for the presence, quantity, and stability of a wide diversity of excreted HAdV and JCPyV. High quantities of HAdV and JCPyV were detected in sewage, effluent wastewater, sludge, and biosolid samples. Both viruses showed high stability in urban sewage. These results confirm the suitability of both viruses as indicators of human fecal viral pollution.

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