Determination of Bacterial Sensitivity to Antibiotics and Pesticide

2017 ◽  
pp. 251-256
Author(s):  
S. G. Borkar
Keyword(s):  
Bacterial Sensitivity

scholarly journals A simple method for the determination of bacterial sensitivity to sulphonamides by the use of blotting-paper disks

1948 ◽  
Vol 46 (4) ◽  
pp. 422-425 ◽  
Author(s):  
R. J. Evans
Keyword(s):  
Red Blood Cells ◽  
Blood Cells ◽  
Nutrient Agar ◽  
Zone Of Inhibition ◽  
Simple Method ◽  
Blotting Paper ◽  
Inhibition Of Growth ◽  
Bacterial Sensitivity ◽  
Horse Red Blood Cells

A description is given of a simple method of determining the sensitivity of bacteria to sulphonamides by means of impregnated blotting-paper disks applied to nutrient agar prepared from broth freed from sulphonamide antagonists by incubation with lysed horse red blood cells.With organisms sensitive to the selected sulphonamide, a zone of inhibition of growth will occur, the diameter of which is related to the concentration of the sulphonamide necessary to inhibit growth. Sensitivity to several sulphonamides can be determined simultaneously.The impregnated disks can be stored without loss of potency.

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