The objective of this research was to investigate the effect of incubation temperature towards the growth of Acetobacter xylinum 0416 and the production of bacterial cellulose. Fermentation of A.xylinum 0416 were carried out for 5 days in static condition using Hestrin & Schramm (HS) medium with initial glucose concentration of 4.0% (w/v) and initial pH of 6.4. The incubation temperature were varied at 5°C, 20°C, 25°C, 27°C, 28°C, 30°C, 35°C and 40°C respectively. Results indicated that 28°C served as the best incubation temperature for the growth of A.xylinum 0416 and produced the highest amount of bacterial cellulose with total dried weight of 0.3722 g/l. Meanwhile at incubation temperature of 5°C and 40°C, no significant growth of A.xylinum 0416 and bacterial cellulose were obtained. As a conclusion, incubation temperature plays an important role for the growth of A.xylinum 0416. The best incubation temperature is at 28°C in which the sufficient energy will be provided for bacterial growth thus enhancing the cellulose biosynthetic pathway in order to convert glucose into bacterial cellulose.
Application of raw starch degrading enzyme from Laceyella sacchari LP175 for development of bacterial cellulose fermentation using colored rice as substrate
