Micro-HPLC

2016 ◽  
pp. 97-120
Keyword(s):  
1985 ◽  
Vol 8 (9) ◽  
pp. 543-546 ◽  
Author(s):  
D. Ishii ◽  
Y. Hashimoto ◽  
H. Asai ◽  
K. Watanabe ◽  
T. Takeuchi
Keyword(s):  

2007 ◽  
Vol 30 (5) ◽  
pp. 669-672 ◽  
Author(s):  
Ricardo Lopez ◽  
Paola Dugo ◽  
Luigi Mondello

1981 ◽  
Vol 4 (10) ◽  
pp. 530-531 ◽  
Author(s):  
A. Hirose ◽  
Y. Iwasaki ◽  
I. Iwata ◽  
K. Ueda ◽  
D. Ishii

1984 ◽  
Vol 17 (10) ◽  
pp. 933-943 ◽  
Author(s):  
Kiyokatsu Jinno
Keyword(s):  

2015 ◽  
Vol 61 (3) ◽  
pp. 556-558 ◽  
Author(s):  
Brian R Stolze ◽  
Verena Gounden ◽  
Jianghong Gu ◽  
Brent S Abel ◽  
Deborah P Merke ◽  
...  
Keyword(s):  

2004 ◽  
Vol 27 (17-18) ◽  
pp. 1467-1474 ◽  
Author(s):  
Yan Li ◽  
Jian Zhang ◽  
Rong Xiang ◽  
Yanhui Yang ◽  
Csaba Horváth

Author(s):  
E. G. Kuznetsova ◽  
O. M. Kuryleva ◽  
L. A. Salomatina ◽  
V. Yu. Belov ◽  
V. I. Sevastianov

Aim: to develop the emulsion transdermal therapeutic system (TTS) of cyclosporine and the research of its diffusion from TTS.Materials and methods. The quantitative evaluation of cyclosporine in model media was performed on liquid chromatograph Agilent 1100 G1311 with spectrophotometric detector G1315B (Germany). For the production of the emulsion matrix, the Heidolph DIAX900 disperser (Germany) and the ultrasonic homogenizer Hielscher UIS250V (Germany) were used. The cyclosporine diffusion from TTS through Strat-M membrane (25 mm in diameter, Merck Millipore) was studied using Copley diffusion analyser (Great Britain).Results. Using the developed method of micro-HPLC, it was established that the amount of cyclosporine passed through the Strat-M membrane over 24 hours from 10 sm2 TTS, containing 173 mg of the substance, was 5.9 mg. The estimated blood concentration is ~ 50 ng/ml which corresponds to the therapeutic maintenance blood concentration during organ transplantation (50–75 ng/ml).Conclusion. The model studies demonstrate the possibility of the cyclosporine transdermal transfer.


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