Dynamic Changes in the Proximal Gut Neural Crest Stem Cell Population Are Associated with Successful Development of the Distal Enteric Nervous System in Rats

The development of the enteric nervous system (ENS) is highly modulated by the synchronized interaction between the enteric neural crest cells (ENCCs) and the neural stem cell niche comprising the gut microenvironment. Genetic defects dysregulating the cellular behaviour(s) of the ENCCs result in incomplete innervation and hence ENS dysfunction. Hirschsprung disease (HSCR) is a rare complex neurocristopathy in which the enteric neural crest-derived cells fail to colonize the distal colon. In addition to ENS defects, increasing evidence suggests that HSCR patients may have intrinsic defects in the niche impairing the extracellular matrix (ECM)-cell interaction and/or dysregulating the cellular niche factors necessary for controlling stem cell behaviour. The niche defects in patients may compromise the regenerative capacity of the stem cell-based therapy and advocate for drug- and niche-based therapies as complementary therapeutic strategies to alleviate/enhance niche-cell interaction. Here, we provide a summary of the current understandings of the role of the enteric neural stem cell niche in modulating the development of the ENS and in the pathogenesis of HSCR. Deciphering the contribution of the niche to HSCR may provide important implications to the development of regenerative medicine for HSCR.

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515a DCLK1 Labels a Multipotent and Residual Neural Crest-Derived Stem Cell in the Gut Epithelium and Enteric Nervous System

Gastroenterology ◽

10.1016/s0016-5085(12)60406-5 ◽

2012 ◽

Vol 142 (5) ◽

pp. S-108 ◽

Cited By ~ 1

Author(s):

Michael Quante ◽

Christoph B. Westphalen ◽

Samuel Asfaha ◽

Michael D. Gershon ◽

Timothy C. Wang

Keyword(s):

Nervous System ◽

Stem Cell ◽

Neural Crest ◽

Enteric Nervous System ◽

Gut Epithelium

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Neural crest regionalisation for enteric nervous system formation: Implications for Hirschsprung's disease and stem cell therapy

Developmental Biology ◽

10.1016/j.ydbio.2009.12.014 ◽

2010 ◽

Vol 339 (2) ◽

pp. 280-294 ◽

Cited By ~ 49

Author(s):

Dongcheng Zhang ◽

Inigo M. Brinas ◽

Benjamin J. Binder ◽

Kerry A. Landman ◽

Donald F. Newgreen

Keyword(s):

Nervous System ◽

Stem Cell ◽

Cell Therapy ◽

Neural Crest ◽

Enteric Nervous System ◽

Stem Cell Therapy ◽

Hirschsprung's Disease ◽

Hirschsprung’S Disease ◽

System Formation

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Identification of a human adult cardiac stem cell population with neural crest origin

10.1101/590679 ◽

2019 ◽

Author(s):

Anna Höving ◽

Madlen Merten ◽

Kazuko Elena Schmidt ◽

Isabel Faust ◽

Lucia Mercedes Ruiz-Perera ◽

...

Keyword(s):

Stem Cells ◽

Stem Cell ◽

Neural Crest ◽

Cell Population ◽

Stem Cell Population ◽

Cell Populations ◽

Cardiac Stem Cell ◽

Adult Human ◽

Neural Crest Origin ◽

Stem Cell Populations

ABSTRACTCardiovascular diseases are the major cause of death worldwide, emphasizing the necessity to better understand adult human cardiac cell biology and development. Although the adult heart was considered as a terminally differentiated organ, rare populations of cardiac stem cells (CSCs) have been described so far, with their developmental origin and endogenous function still being a matter of debate.Here, we identified a Nestin+/S100+/CD105+/Sca1+/cKit-population of CSCs in the myocardium of the adult human heart auricle. Isolated cells showed expression of characteristic neural crest-derived stem cell (NCSC) markers and kept their genetic stability during cultivationin vitro. Cultivated hCSCs efficiently gave rise to functional, beating cardiomyocytes, osteoblasts, adipocytes and neurons. Global transcriptome analysis via RNAseq showed a high similarity between the expression profiles of Nestin+/S100+/CD105+/Sca1+/cKit-hCSCs and adult human NCSCs from the nasal cavity (inferior turbinate stem cells, ITSCs). In detail, 88.1 % of all genes were significantly expressed in both stem cell populations particularly including common NCSC-markers. Based on these observations, we suggest a similar developmental origin of both stem cell populations.In summary, we identified a human adult cardiac stem cell population with neural crest-origin, which may also contribute to endogenous cardiac tissue homeostasis and tissue repairin vivo.

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