Structural Analysis and Subunit Interaction of Insulin Receptor from Membranes of Cultured Embryonic Chick Heart Cells*

Endocrinology ◽  
1984 ◽  
Vol 115 (5) ◽  
pp. 1828-1837 ◽  
Author(s):  
FUMIO ENDO ◽  
LOUIS J. ELSAS
Keyword(s):  
Structural Analysis ◽  
Insulin Receptor ◽  
Heart Cells ◽  
Embryonic Chick ◽  
Subunit Interaction ◽  
Chick Heart ◽  
Embryonic Chick Heart

Toxicity of Emetine to Isolated Embryonic Chick-Heart Cells

1968 ◽  
Vol 57 (11) ◽  
pp. 1968-1974 ◽  
Author(s):  
W. David Watkins ◽  
Wallace L. Guess
Keyword(s):  
Heart Cells ◽  
Embryonic Chick ◽  
Chick Heart ◽  
Embryonic Chick Heart

scholarly journals The Electrical Activity of Embryonic Chick Heart Cells Isolated in Tissue Culture Singly or in Interconnected Cell Sheets

1968 ◽  
Vol 52 (3) ◽  
pp. 643-665 ◽  
Author(s):  
Robert L. DeHaan ◽  
Sheldon H. Gottlieb
Keyword(s):  
Action Potential ◽  
Single Cells ◽  
Electrical Contact ◽  
Resting Potential ◽  
Heart Cells ◽  
Embryonic Chick ◽  
Irreversible Damage ◽  
Diastolic Depolarization ◽  
Chick Heart ◽  
Embryonic Chick Heart

Embryonic chick heart cells were cultured on a plastic surface in sparse sheets of 2–50 cells mutually in contact, or isolated as single cells. Conditions are described which permitted conjoint cells to be impaled with recording microelectrodes with 75% success, and isolated single cells with 8% success. It is proposed that cells in electrical contact with neighbors are protected from irreversible damage by the penetrating electrode, by a flow of ions or other substances from connected cells across low-impedance intercellular junctions. Action potentials recorded from conjoint and isolated single cells were similar in form and amplitude. The height or shape of the action potential thus appears not to depend upon spatial relationships of one cell to another. As the external potassium concentration was increased from 1.3 mM to 6 mM, cells became hyperpolarized while the afterhyperpolarization was reduced. At higher potassium levels, the afterhyperpolarization disappeared, the slope of the slow diastolic depolarization decreased, and resting potential fell along a linear curve with a slope of 61 mv per 10-fold increase in potassium. In pacemaker cells the diastolic depolarization consists of two phases: (a) recovery from the afterpotential of the previous action potential and (b) the pacemaker potential. These phases are separated by a point of inflection, and represent manifestations of different mechanisms. Evidence is presented that it is the point of inflection (PBA) rather than the point of maximal diastolic potential, that should be taken as the resting potential.

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