Phorbol ester stimulates the synthesis and secretion of brain natriuretic peptide from neonatal rat ventricular cardiocytes: a comparison with the regulation of atrial natriuretic factor

1993 ◽  
Vol 7 (10) ◽  
pp. 1284-1296 ◽  
Author(s):  
M. C. LaPointe
Keyword(s):  
Brain Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Phorbol Ester ◽  
Atrial Natriuretic Factor ◽  
Neonatal Rat ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

The expression and secretion of atrial natriuretic factor and brain natriuretic peptide by rat proximal tubular cells

2000 ◽  
Vol 59 (7) ◽  
pp. 783-790 ◽  
Author(s):  
Sanjay Mistry ◽  
Benedict Lussert ◽  
Keith Stewart ◽  
Gabrielle M Hawksworth ◽  
Alan Struthers ◽  
...  
Keyword(s):  
Brain Natriuretic Peptide ◽  
Natriuretic Peptide ◽  
Atrial Natriuretic Factor ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Natriuretic Factor ◽  
Proximal Tubular ◽  
Atrial Natriuretic

Evidence for Atrial Natriuretic Factor Induced Natriuretic Peptide Receptor Subtype Switching in Rat Proximal Tubular Cells during Culture

1998 ◽  
Vol 6 (2) ◽  
pp. 104-111 ◽  
Author(s):  
Sanjay K. Mistry ◽  
Prabal K. Chatterjee ◽  
Roshan P. Weerackody ◽  
Gabrielle M. Hawksworth ◽  
Rachel M. Knott ◽  
...  
Keyword(s):  
Natriuretic Peptide ◽  
Atrial Natriuretic Factor ◽  
Receptor Subtype ◽  
Natriuretic Peptide Receptor ◽  
Proximal Tubular Cells ◽  
Tubular Cells ◽  
Peptide Receptor ◽  
Natriuretic Factor ◽  
Proximal Tubular ◽  
Atrial Natriuretic

Regulated secretion of atrial natriuretic factor from cultured ventricular myocytes

1993 ◽  
Vol 264 (1) ◽  
pp. H282-H285 ◽  
Author(s):  
C. E. Irons ◽  
C. A. Sei ◽  
C. C. Glembotski
Keyword(s):  
Transit Time ◽  
Atrial Natriuretic Factor ◽  
Ventricular Myocytes ◽  
Secretory Granules ◽  
Subcellular Fractionation ◽  
Neonatal Rat ◽  
Cell Type ◽  
Regulated Secretion ◽  
Natriuretic Factor ◽  
Atrial Natriuretic

We have investigated endothelin (ET)-regulated secretion of atrial natriuretic factor (ANF) from primary neonatal rat ventricular myocytes, where hormone release is thought to be constitutive. In a dose-dependent, nifedipine-sensitive manner, ET acutely enhanced ANF release by two- to fivefold over control cultures within 15 min of agonist exposure, demonstrating that ventricular myocytes display a primary characteristic of a regulated secretory cell type. Unlike atrial cultures, ET enhanced ANF release during the first 30 min of exposure; thereafter, secretion rates returned to control levels. KCl, however, effectively enhanced ANF release only during the first 15 min of exposure. Subcellular fractionation of ventricular culture homogenates did not reveal atrial-type dense secretory granules, and pulse-chase labeling experiments showed that the transit time of newly synthesized ANF was short in ventricular myocytes [time required for half of labeled ANF to be released from cells (t1/2) = 0.5-1.5 h) compared with atrial myocytes (t1/2 = 4 h). These results suggest that, whereas ventricular myocytes possess some of the characteristics of a constitutively secreting cell type (e.g., few, if any, dense secretory granules and rapid transit time for newly synthesized hormone); however, they also display the capacity for regulated secretion of ANF in response to the physiological agonist ET.

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