Molecular diversity and functional characterization of voltage- dependent calcium channels (CACN4) expressed in pancreatic beta-cells

1995 ◽  
Vol 9 (1) ◽  
pp. 121-130 ◽  
Author(s):  
Y. Ihara
Keyword(s):  
Calcium Channels ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Molecular Diversity ◽  
Functional Characterization ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels

scholarly journals Identification and Functional Characterization of Voltage-dependent Calcium Channels in T Lymphocytes

2003 ◽  
Vol 278 (47) ◽  
pp. 46949-46960 ◽  
Author(s):  
Maya F. Kotturi ◽  
Douglas A. Carlow ◽  
Junella C. Lee ◽  
Hermann J. Ziltener ◽  
Wilfred A. Jefferies
Keyword(s):  
T Lymphocytes ◽  
Calcium Channels ◽  
Functional Characterization ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels

scholarly journals Ascorbic acid modulation of calcium channels in pancreatic beta cells.

1993 ◽  
Vol 102 (3) ◽  
pp. 503-523 ◽  
Author(s):  
R V Parsey ◽  
D R Matteson
Keyword(s):  
Ascorbic Acid ◽  
Calcium Channels ◽  
Beta Cells ◽  
Pancreatic Beta Cells ◽  
Inactivation Kinetics ◽  
Dependent Manner ◽  
Patch Clamp Technique ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels ◽  
Metal Catalyzed

We have studied the effect of ascorbic acid on voltage-dependent calcium channels in pancreatic beta cells. Using the whole-cell and perforated-patch variants of the patch clamp technique to record calcium tail currents, we have shown that the slowly deactivating (SD) calcium channel, which is similar to the T-type channel in other cells, is inhibited in a voltage-dependent manner by ascorbic acid (AA). The other channels that carry inward current in beta cells, FD calcium channels and sodium channels, are unaffected by AA. Ascorbic acid causes a voltage-dependent decrease in the magnitude of the SD channel conductance which can be explained by the hypothesis that approximately 50-60% of the channels have their voltage dependence shifted by approximately 62 mV in the depolarizing direction. Thus, ascorbate appears to modify only a fraction of the SD channels. The activation kinetics of the ascorbate-modified channels are slower than control channels in a manner that is consistent with this hypothesis. Deactivation and inactivation kinetics are unaffected by ascorbate. These effects of ascorbate require metal ions, and it appears that some of the activity of ascorbate is due to a product of its metal catalyzed oxidation, perhaps dehydroascorbate.

Characterization of Novel Probes of Voltage-Dependent Calcium Channels

1990 ◽  
pp. 59-66
Author(s):  
Gregory J. Kaczorowski ◽  
John P. Felix ◽  
Maria L. Garcia ◽  
V. Frank King ◽  
Judith L. Shevell ◽  
...  
Keyword(s):  
Calcium Channels ◽  
Voltage Dependent ◽  
Voltage Dependent Calcium Channels
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