Interactions between rhombomeres modulate Krox-20 and follistatin expression in the chick embryo hindbrain

Development ◽  
1996 ◽  
Vol 122 (2) ◽  
pp. 473-480 ◽  
Author(s):  
A. Graham ◽  
A. Lumsden
Keyword(s):  
Chick Embryo ◽  
Neural Crest ◽  
Cell Lineage ◽  
Signalling Molecule ◽  
Neighbour Interaction

The rhombomeres of the embryonic hindbrain display compartment properties, including cell lineage restriction, genetic definition and modular anatomical phenotype. Consistent with the idea that rhombomeres are autonomous developmental units, previous studies have shown that certain aspects of rhombomere phenotype are determined early, at the time of rhombomere formation. By contrast, the apoptotic depletion of neural crest from rhombomeres 3 and 5 is due to an interaction with their neighbouring rhombomeres, involving the signalling molecule Bmp4. In this paper, we have examined whether inter-rhombomere interactions control further aspects of rhombomere phenotype. We find that the expression of Krox-20 and the repression of follistatin in r3 is dependent upon neighbour interaction, whereas these genes are expressed autonomously in r5. We further demonstrate that modulation of Krox-20 and follistatin expression is not dependent on Bmp4, indicating the existence of multiple pathways of interaction between adjacent rhombomeres. We also show that, although some phenotypic aspects of r3 are controlled by neighbour interactions, the axial identity of the segment is intrinsically determined.

Cell lineage analysis in neural crest ontogeny

1993 ◽  
Vol 24 (2) ◽  
pp. 146-161 ◽  
Author(s):  
Nicole M. Le Douarin ◽  
Elisabeth Dupin
Keyword(s):  
Neural Crest ◽  
Cell Lineage ◽  
Lineage Analysis

Non-malignant migration of B16 mouse melanoma cells in the neural crest and invasive growth in the eye cup of the chick embryo

2007 ◽  
Vol 17 (1) ◽  
pp. 17-30 ◽  
Author(s):  
Matthias Oppitz ◽  
Christian Busch ◽  
Gernot Schriek ◽  
Marco Metzger ◽  
Lothar Just ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Neural Crest ◽  
Melanoma Cells ◽  
Invasive Growth ◽  
Mouse Melanoma ◽  
B16 Mouse Melanoma

Segmentation in the chick embryo hindbrain is defined by cell lineage restrictions

Nature ◽  
1990 ◽  
Vol 344 (6265) ◽  
pp. 431-435 ◽  
Author(s):  
Scott Fraser ◽  
Roger Keynes ◽  
Andrew Lumsden
Keyword(s):  
Chick Embryo ◽  
Cell Lineage

Overexpression of Snail family members highlights their ability to promote chick neural crest formation

Development ◽  
2002 ◽  
Vol 129 (7) ◽  
pp. 1583-1593 ◽  
Author(s):  
Marta G. del Barrio ◽  
M. Angela Nieto
Keyword(s):  
Chick Embryo ◽  
Neural Crest ◽  
Neural Tube ◽  
Family Members ◽  
Ectopic Expression ◽  
Endogenous Gene ◽  
The Family ◽  
Snail Family ◽  
Migratory Cells ◽  
Snail Gene

The Snail gene family of transcription factors plays crucial roles in different morphogenetic processes during the development of vertebrate and invertebrate embryos. In previous studies of function interference for one of the family members, Slug, we showed its involvement and neural crest formation in the chick embryo. Now we have carried out a series of gain-of-function experiments in which we show that Slug overexpression in the neural tube of the chick embryo induces an increase in neural crest production. The analysis of electroporated embryos shows that Slug can induce the expression of rhoB and an increase in the number of HNK-1-positive migratory cells, indicating that it lies upstream of them in the genetic cascade of neural crest development. The increase in neural crest production after Slug overexpression was confined to the cranial region, indicating that the mechanisms of crest induction somehow differ between head and trunk. The expression of the two vertebrate family members, Slug and Snail, is peculiar with respect to the neural crest. Slug is not expressed in the premigratory crest in the mouse, whereas it is expressed in this cell population in the chick and the opposite is true for Snail(Sefton, M., Sánchez, S. and Nieto M. A. (1998) Development125, 3111-3121). This raises the question of whether they can be functionally equivalent. To test this hypothesis both intra- and interspecies, we have performed a series of ectopic expression experiments by electroporating chick and mouse Snail in the chick embryo hindbrain. We observe that both genes elicit the same responses in the neural tube. Our results indicate that they can be functionally equivalent, although the embryos show a higher response to the endogenous gene, chick Slug.

scholarly journals Development of the Schwann cell lineage: From the neural crest to the myelinated nerve

Glia ◽  
2008 ◽  
Vol 56 (14) ◽  
pp. 1481-1490 ◽  
Author(s):  
Ashwin Woodhoo ◽  
Lukas Sommer
Keyword(s):  
Schwann Cell ◽  
Neural Crest ◽  
Cell Lineage ◽  
Myelinated Nerve

The signalling molecule BMP4 mediates apoptosis in the rhombencephalic neural crest

Nature ◽  
1994 ◽  
Vol 372 (6507) ◽  
pp. 684-686 ◽  
Author(s):  
Anthony Graham ◽  
Philippa Francis-West ◽  
Paul Brickell ◽  
Andrew Lumsden
Keyword(s):  
Neural Crest ◽  
Signalling Molecule

Segmental origin and migration of neural crest cells in the hindbrain region of the chick embryo

Development ◽  
1991 ◽  
Vol 113 (4) ◽  
pp. 1281-1291 ◽  
Author(s):  
A. Lumsden ◽  
N. Sprawson ◽  
A. Graham
Keyword(s):  
Chick Embryo ◽  
Neural Crest ◽  
Fate Map ◽  
Membrane Probe ◽  
Vital Dye ◽  
Video Fluorescence Microscopy ◽  
Dye Analysis ◽  
And Migration ◽  
Neural Crest Migration ◽  
Branchial Region

A vital dye analysis of cranial neural crest migration in the chick embryo has provided a positional fate map of greater resolution than has been possible using labelled graft techniques. Focal injections of the fluorescent membrane probe DiI were made into the cranial neural folds at stages between 3 and 16 somites. Groups of neuroepithelial cells, including the premigratory neural crest, were labelled by the vital dye. Analysis of whole-mount embryos after 1–2 days further development, using conventional and intensified video fluorescence microscopy, revealed the pathways of crest cells migrating from mesencephalic and rhombencephalic levels of the neuraxis into the subjacent branchial region. The patterns of crest emergence and emigration correlate with the segmented disposition of the rhombencephalon. Branchial arches 1, 2 and 3 are filled by crest cells migrating from rhombomeres 2, 4 and 6 respectively, in register with the cranial nerve entry/exit points in these segments. The three streams of ventrally migrating cells are separated by alternating regions, rhombomeres 3 and 5, which release no crest cells. Rostrally, rhombomere 1 and the caudal mesencephalon also contribute crest to the first arch, primarily to its upper (maxillary) component. Both r3 and r5 are associated with enhanced levels of cell death amongst cells of the dorsal midline, suggesting that crest may form at these levels but is then eliminated. Organisation of the branchial region is thus related by the dynamic process of neural crest immigration to the intrinsic mechanisms that segment the neuraxis.

Sign in / Sign up

Export Citation Format

Share Document