The effect of biopreparations of nodules bacteria with carmoisine on the formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic systems

Purpose. Investigate the formation and functioning of symbiotic systems of soybeans with nodule bacteria by ino­culation of seeds with biological products based on fungicide-resistant strains of Bradyrhizobium japonicum PC07 and B78 with different rates of synthetic carmoisine colorant. Methods. Physiological, microbiological, gas chromatography, statistical. Results. It was found that as a result of inoculation of soybean [Glycine max (L.) Merr] variety ‘Almaz’ with microbial preparations based on B. japonicum PC07 and B78, with the addition of carmoisine (0.25 and 0.5 g per 200 g of the preparation), the amount and the weight of nodules formed on the roots during the growing season were at the level of the control plants or exceeded them. The greatest difference in indicators of quantity and weight of root nodules between plants of control and experimental variants is noted in a phase of full flowering at inoculation by both strains of rhizobia and addition to biological products of various norms of dye. Analysis of nitrogen-fixing activity (NFA) of the formed symbiotic systems showed the absence of a negative effect of the synthetic colorant on its level. When inoculated with soybean seeds B. japonicum PC07 in the phase of three true leaves, NFA was higher by 15.6–25.9% and in the budding-beginning of flowering stage by 7.4–29.5% compared with control plants with the addition of 0.25 and 0.5 g of carmoisine, respectively. Against the background of bacterization of soybean seeds by strain B78 before the phase of full flowering of plants the level of N2 assimilation by adding 0.25 g of carmoisine to the vermiculite preparation was at the level of the control plants. During the period of full flowering, this figure exceeded the indicators of control plants by 7.6 and 18.8% with the introduction of 0.25 and 0.5 g of the colorant. Conclusions. Carmoisine can be applied in the further study of the effectiveness of its use as a dye identifier for controlling the uniformity of marking of loose bacterial preparations on seeds by adding 0.25 and 0.5 g per 200 g of a biopreparation, since this did not show a negative impact on the formation and functioning of the soybean – Bradyrhizobium japonicum symbiotic systems.

An optimised small-scale sample preparation workflow for historical dye analysis using UHPLC-PDA applied to Scottish and English Renaissance embroidery

A sample preparation workflow for historical dye analysis based on 96 well plates and filtration by centrifugation was developed. It requires less sample and the introduced error is decreased, making it useful for culturally important objects.

A composite polymer of polystyrene coated with poly(4-vinylpyridine) as a sorbent for the extraction of synthetic dyes from foodstuffs

A poly(St-co-EGDMA)@poly(4-vinylpyridine-co-EGDMA) composite polymer enables sample pre-treatment in food dye analysis in an environment friendly and economical manner.

Analisis Bahan Pewarna Sintetis Non Pangan Rhodamin B dan Methanyl Yellow pada Produk Saus Tomat dan Saus Cabe di Kota Banda Aceh

Abstrak.Penelitian ini bertujuan untuk mengidentifikasi bahan tambahan pewarna non pangan Rhodamin B dan Methanyl Yellow pada produk saus tomat dan saus cabe di kota Banda Aceh. Penelitian ini menggunakan metode penelitian survei konsumen dan analisis pewarna. Survei konsumen dilakukan dengan teknik incidental sampling yaitu penentuan responden berdasarkan kebetulan, yaitu siapa saja yang membeli produk saus dapat dijadikan sebagai responden. sedangkan analisis pewarna dilakukan secara kualitatif yaitu mengidentifikasi ada tidaknya kandungan bahan tambahan pewarna Rhodamin B dan Methanyl Yellow pada tiap sampel. Pada uji kualitatif zat pewarna Rhodamin B dan Methanyl Yellow diperoleh hasil bahwa tidak terdeteksi adanya kandungan zat pewarna Rhodamin B dan Methanyl Yellow pada keenam sampel saus tomat yang dianalisis.Abstract.This study aims to identify non-food coloring additives of Rhodamin B and Methanyl Yellow in tomato sauce and chilli sauce products in the city of Banda Aceh. This research used consumer survey research methods and dye analysis. Consumer survey was done by incidental sampling technique in which the determination of respondent is decided by chance so that anyone bought sauce product can be made as respondent. While the dye analysis was conducted qualitatively, the presence of additive material of Rhodamin B and Methanyl Yellow dyes in each sample was identified. In the qualitative test of Rhodamin B and Methanyl Yellow, it was found that no detectable content of Rhodamin B and Methanyl Yellow dyes in the six samples of tomato sauce analyzed.