scholarly journals The retromer component sorting nexin-1 is required for efficient retrograde transport of Shiga toxin from early endosome to the trans Golgi network

2007 ◽  
Vol 120 (12) ◽  
pp. 2010-2021 ◽  
Author(s):  
M. V. Bujny ◽  
V. Popoff ◽  
L. Johannes ◽  
P. J. Cullen
Keyword(s):  
Shiga Toxin ◽  
Retrograde Transport ◽  
Early Endosome ◽  
Sorting Nexin ◽  
Trans Golgi Network ◽  
Golgi Network ◽  
Sorting Nexin 1

scholarly journals Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport

2017 ◽  
Vol 216 (11) ◽  
pp. 3677-3693 ◽  
Author(s):  
Arunas Kvainickas ◽  
Ana Jimenez-Orgaz ◽  
Heike Nägele ◽  
Zehan Hu ◽  
Jörn Dengjel ◽  
...  
Keyword(s):  
Quantitative Proteomics ◽  
Retrograde Transport ◽  
Dependent Manner ◽  
Sorting Nexin ◽  
Trans Golgi Network ◽  
The Core ◽  
Retromer Complex ◽  
Mannose 6 Phosphate ◽  
Golgi Network

The retromer complex, which recycles the cation-independent mannose 6-phosphate receptor (CI-MPR) from endosomes to the trans-Golgi network (TGN), is thought to consist of a cargo-selective VPS26–VPS29–VPS35 trimer and a membrane-deforming subunit of sorting nexin (SNX)–Bin, Amphyphysin, and Rvs (BAR; SNX-BAR) proteins. In this study, we demonstrate that heterodimers of the SNX-BAR proteins, SNX1, SNX2, SNX5, and SNX6, are the cargo-selective elements that mediate the retrograde transport of CI-MPR from endosomes to the TGN independently of the core retromer trimer. Using quantitative proteomics, we also identify the IGF1R, among more potential cargo, as another SNX5 and SNX6 binding receptor that recycles through SNX-BAR heterodimers, but not via the retromer trimer, in a ligand- and activation-dependent manner. Overall, our data redefine the mechanics of retromer-based sorting and call into question whether retromer indeed functions as a complex of SNX-BAR proteins and the VPS26–VPS29–VPS35 trimer.

scholarly journals The Golgin GCC88 Is Required for Efficient Retrograde Transport of Cargo from the Early Endosomes to the Trans-Golgi Network

2007 ◽  
Vol 18 (12) ◽  
pp. 4979-4991 ◽  
Author(s):  
Zi Zhao Lieu ◽  
Merran C. Derby ◽  
Rohan D. Teasdale ◽  
Charles Hart ◽  
Priscilla Gunn ◽  
...  
Keyword(s):  
Shiga Toxin ◽  
Retrograde Transport ◽  
Transport Pathway ◽  
Transport Pathways ◽  
Recycling Endosomes ◽  
Trans Golgi Network ◽  
Early Endosomes ◽  
Cargo Proteins ◽  
Mannose 6 Phosphate ◽  
Golgi Network

Retrograde transport pathways from early/recycling endosomes to the trans-Golgi network (TGN) are poorly defined. We have investigated the role of TGN golgins in retrograde trafficking. Of the four TGN golgins, p230/golgin-245, golgin-97, GCC185, and GCC88, we show that GCC88 defines a retrograde transport pathway from early endosomes to the TGN. Depletion of GCC88 in HeLa cells by interference RNA resulted in a block in plasma membrane–TGN recycling of two cargo proteins, TGN38 and a CD8 mannose-6-phosphate receptor cytoplasmic tail fusion protein. In GCC88-depleted cells, cargo recycling was blocked in the early endosome. Depletion of GCC88 dramatically altered the TGN localization of the t-SNARE syntaxin 6, a syntaxin required for endosome to TGN transport. Furthermore, the transport block in GCC88-depleted cells was rescued by syntaxin 6 overexpression. Internalized Shiga toxin was efficiently transported from endosomes to the Golgi of GCC88-depleted cells, indicating that Shiga toxin and TGN38 are internalized by distinct retrograde transport pathways. These findings have identified an essential role for GCC88 in the localization of TGN fusion machinery for transport from early endosomes to the TGN, and they have allowed the identification of a retrograde pathway which differentially selects TGN38 and mannose-6-phosphate receptor from Shiga toxin.

GGA proteins regulate retrograde transport of BACE1 from endosomes to the trans-Golgi network

2005 ◽  
Vol 29 (3) ◽  
pp. 453-461 ◽  
Author(s):  
Tina Wahle ◽  
Kai Prager ◽  
Nikolai Raffler ◽  
Christian Haass ◽  
Michael Famulok ◽  
...  
Keyword(s):  
Retrograde Transport ◽  
Trans Golgi Network ◽  
Golgi Network

scholarly journals Yeast synaptobrevin, Snc1, engages distinct routes of postendocytic recycling mediated by a sorting nexin, Rcy1-COPI, and retromer

2020 ◽  
Vol 31 (9) ◽  
pp. 944-962
Author(s):  
Jordan T. Best ◽  
Peng Xu ◽  
Jack G. McGuire ◽  
Shannon N. Leahy ◽  
Todd R. Graham
Keyword(s):  
Plasma Membrane ◽  
Multiple Modes ◽  
Sorting Nexin ◽  
Trans Golgi Network ◽  
Golgi Network

The yeast synaptobrevin, Snc1, uses multiple modes of postendocytic recycling to facilitate its return to either the trans-Golgi network or the plasma membrane. Snc1 primarily recycles via pathways dependent on either Rcy1/COPI or Snx4, while a smaller portion of the SNARE appears to be capable of being retrieved by retromer.

BFA-induced compartments from the Golgi apparatus and trans-Golgi network/early endosome are distinct in plant cells

2009 ◽  
Vol 60 (5) ◽  
pp. 865-881 ◽  
Author(s):  
Sheung Kwan Lam ◽  
Yi Cai ◽  
Yu Chung Tse ◽  
Juan Wang ◽  
Angus Ho Yin Law ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Plant Cells ◽  
Early Endosome ◽  
Trans Golgi Network ◽  
Golgi Network
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