Redistribution of a Golgi glycoprotein in plant cells treated with Brefeldin A

1992 ◽  
Vol 103 (4) ◽  
pp. 1153-1166 ◽  
Author(s):  
B. Satiat-Jeunemaitre ◽  
C. Hawes
Keyword(s):  
Golgi Apparatus ◽  
Fatty Acid Derivative ◽  
Plant Cells ◽  
Brefeldin A ◽  
Root Tip ◽  
Animal Cells ◽  
Higher Plant ◽  
Onion Root ◽  
Tip Cells ◽  
Root Tip Cells

The fungal fatty acid derivative Brefeldin A (BFA), has been used to study the reversible distribution of a Golgi glycoprotein, the JIM 84 epitope, into the cytosol of higher plant cells. Treatment of both maize and onion root tip cells resulted in a rearrangement of the Golgi stacks into either circular formations or a perinuclear distribution. The Golgi cisternae became curved and vesiculated and in cells where the Golgi apparatus was totally dispersed the JIM 84 epitope was associated with large areas in the cytosol which were also vesiculated. On removal of the BFA the Golgi apparatus reformed and the JIM 84 epitope was again located in the cisternal stacks. This mode of BFA action is compared with that so far described for animal cells.

Brefeldin A affects the endomembrane system and vesicle trafficking in higher plants

1993 ◽  
Vol 51 ◽  
pp. 192-193
Author(s):  
Béatrice Satiat-Jeunemaitre ◽  
Jancy Henderson ◽  
David Evans ◽  
Kim Crooks ◽  
Mark Fricker ◽  
...  
Keyword(s):  
Golgi Apparatus ◽  
Higher Plants ◽  
Fatty Acid Derivative ◽  
Plant Cells ◽  
Brefeldin A ◽  
Endomembrane System ◽  
Animal Cells ◽  
Higher Plant ◽  
Membrane Flow ◽  
Golgi Stack

In plant cells, as in animal cells, many macromolecules and membranes are transported by vesicle vectors through both the exocytotic and endocytotic pathways. In order to elucidate the mechanisms and molecular events of such trafficking we are using a set of drugs known to perturb membrane flow in plant cells in combination with immunocytochemical studies using a bank of monoclonal antibodies to various components of the endomembrane system and cell surface. In animal cells, one such drug, Brefeldin A, a fungal fatty acid derivative which causes disruption of the Golgi apparatus, has recently been used as a tool to dissect the mechanisms of vesicle flow from the endoplasmic reticulum to the Golgi apparatus and down the cisternae of the Golgi stack (1). It has been demonstrated that BFA also has a dramatic effect on the Golgi apparatus in higher plant cells (2,3,4).In this paper we report on recent work on the disruption of the plant Golgi apparatus with BFA and the redistribution of endomembrane marker epitopes after drug treatment of roots and suspension culture cells.

INATE AND PHOTO-CONTROLLED MITOTIC RHYTHMS IN ONION ROOT-TIP CELLS

1973 ◽  
Vol 15 (4) ◽  
pp. 667-670 ◽  
Author(s):  
Richard C. Bishop ◽  
Richard M. Klein
Keyword(s):  
Mitotic Activity ◽  
Diurnal Rhythm ◽  
Low Temperatures ◽  
Root Tip ◽  
Fluorescent Light ◽  
Onion Root ◽  
Seed Imbibition ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Red Radiation

A four-peaked diurnal rhythm in mitotic activity of dark-grown onion root-tip cells is initiated upon seed imbibition, damps rapidly and is lost within 5 days. It is abolished by continuous white fluorescent light, by continuous blue, green or red radiation, by low temperatures, high osmotica or anoxia. Photoperiodic light controls the well-known two-peaked persistent rhythm. The nature of the zeitgeber for either the inate or the photoperiodically-controlled rhythms is unknown.

Depolymerization of microtubule arrays in root tip cells by oryzalin and their recovery with modified nucleation patterns

1988 ◽  
Vol 66 (12) ◽  
pp. 2353-2366 ◽  
Author(s):  
A. L. Cleary ◽  
A. R. Hardham
Keyword(s):  
Immunofluorescence Microscopy ◽  
Root Tip ◽  
Cell Cortex ◽  
Microtubule Depolymerization ◽  
Higher Plant ◽  
Resistant Plant ◽  
Normal Functional ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Preprophase Bands

Immunofluorescence microscopy with anti-tubulin has been used to study the effects of the dinitroaniline herbicide, oryzalin, on microtubule arrays in root tip cells of a number of species of plants. All species of grasses that were examined showed rapid microtubule depolymerization, as did the nongrass Potamogeton. All other nongrass monocotyledons and dicotyledons tested required much longer treatment times to achieve microtubule depolymerization in the majority of root tip cells. Correlated immunofluorescence and electron microscopy have been used to obtain details of depolymerization and repolymerization of microtubules during treatment and recovery in a resistant plant, Zinnia elegans, and in a sensitive plant, Lolium rigidum. Although rates of disruption differ, both plants displayed similar patterns of microtubule depolymerization and repolymerization. The microtubules that comprise the different categories of array in the root tip cells were differentially sensitive to oryzalin. In order of increasing stability they were as follows: polar microtubules in mitotic cells < interphase arrays < preprophase bands ≤ phragmoplasts [Formula: see text] kinetochore microtubules. During recovery, microtubules are nucleated in the cell cortex in interphase and at the kinetochores during mitosis. The cells are able to reinstate cortical interphase arrays, preprophase bands, and phragmoplasts of apparently normal organization, but not normal, functional spindles. Our results provide basic information on the use of oryzalin in studying the organization and dynamics of microtubule arrays in higher plant cells.

Cytological investigations on onion root-tip cells treated with some cigarette smoke constituents

1974 ◽  
Vol 65 (5) ◽  
pp. 311-313 ◽  
Author(s):  
P. R. BHALLA ◽  
R. C. ARNOLD ◽  
P. S. SABHARWAL
Keyword(s):  
Cigarette Smoke ◽  
Root Tip ◽  
Onion Root ◽  
Tip Cells ◽  
Root Tip Cells

Effect of Reserpine Phosphate on Dividing Onion Root-tip Cells

1958 ◽  
Vol 98 (2) ◽  
pp. 252-254 ◽  
Author(s):  
M. R. Grimm ◽  
F. C. Rull ◽  
R. L. Mayer
Keyword(s):  
Root Tip ◽  
Onion Root ◽  
Tip Cells ◽  
Root Tip Cells

C3-P-15Spatio-temporal differences between RanGAP and microtubule bands during the development of preprophase bands in onion root tip cells

Microscopy ◽  
2015 ◽  
Vol 64 (suppl 1) ◽  
pp. i133.2-i133
Author(s):  
Takatoshi Yabuuchi ◽  
Tomonori Nakai ◽  
Daisuke Yamauchi ◽  
Seiji Sonobe ◽  
Yoshinobu Mineyuki
Keyword(s):  
Root Tip ◽  
Onion Root ◽  
Temporal Differences ◽  
Tip Cells ◽  
Root Tip Cells ◽  
Preprophase Bands

scholarly journals AN ATYPICAL CRISTA RESEMBLING A "TIGHT JUNCTION" IN BEAN ROOT MITOCHONDRIA

1968 ◽  
Vol 39 (1) ◽  
pp. 35-42 ◽  
Author(s):  
Eldon H. Newcomb ◽  
Martin W. Steer ◽  
Peter K. Hepler ◽  
William P. Wergin
Keyword(s):  
Phaseolus Vulgaris ◽  
Tight Junction ◽  
Tight Junctions ◽  
Osmium Tetroxide ◽  
Root Tip ◽  
Mitochondrial Matrix ◽  
Phaseolus Vulgaris L ◽  
Animal Cells ◽  
Tip Cells ◽  
Root Tip Cells

The conformation and structure of an atypical crista found in a small percentage of the mitochondria in root tip cells of Phaseolus vulgaris L. have been studied electron microscopically in material fixed in glutaraldehyde followed by osmium tetroxide. In its transformation into an atypical crista, a normal crista elongates, broadens, and flattens, and the inner leaflets of its apposed unit membranes appear to fuse in a manner analogous to the formation of "tight junctions" between certain animal cells. The result is a large platelike, quintuple-layered structure, 240–260 A thick, whose long axis parallels that of the mitochondrion. The outer layers of the "plate," bordering on the mitochondrial matrix, are thickened and exhibit striking patterns in the micrographs. The structure of the plate is compared with that previously described for tight junctions between animal cells.

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