Complementation of G1-phase variants of a mammalian cell cycle

1979 ◽  
Vol 35 (1) ◽  
pp. 53-58
Author(s):  
P.M. Naha
Keyword(s):  
Cell Cycle ◽  
Cell Fusion ◽  
Mammalian Cell ◽  
S Phase ◽  
G1 Phase ◽  
Temperature Sensitive ◽  
Multinucleated Cells ◽  
Mammalian Cell Cycle ◽  
Complementation Groups ◽  
Phase Variants

Complementation between temperature-sensitive (ts) variants of Balb/c-3T3 defective in the G1 phase of its cell cycle was measured in the [3H]thymidine-labeling indices of the multinucleated cells during incubation at the restricted temperature (38 degrees C) following cell fusion. One ts variant from each group along the length of the G1 phase was tested for complementation. Varying degrees of complementation were observed between the 4 ts variants tested, judging by the time of entry into S-phase and the degree of synchrony attained. At least 3 complementation groups were discernible.

scholarly journals Underlying principles of cell fate determination during G1 phase of the mammalian cell cycle

Cell Cycle ◽  
2008 ◽  
Vol 7 (20) ◽  
pp. 3246-3257 ◽  
Author(s):  
Benjamin Pfeuty ◽  
Thérèse David-Pfeuty ◽  
Kunihiko Kaneko
Keyword(s):  
Cell Cycle ◽  
Cell Fate ◽  
Mammalian Cell ◽  
G1 Phase ◽  
Cell Fate Determination ◽  
Fate Determination ◽  
Mammalian Cell Cycle

Use of a cell cycle mutant to delineate the critical period for the control of histone mRNA levels in the mammalian cell cycle

1984 ◽  
Vol 4 (11) ◽  
pp. 2364-2369
Author(s):  
A Artishevsky ◽  
A M Delegeane ◽  
A S Lee
Keyword(s):  
Cell Cycle ◽  
Critical Period ◽  
Fibroblast Cell ◽  
Temporal Analysis ◽  
S Phase ◽  
Temperature Sensitive ◽  
Mrna Levels ◽  
Histone Mrna ◽  
Mrna Accumulation ◽  
Mammalian Cell Cycle

Temporal analysis of DNA replication and histone mRNA accumulation in a hamster fibroblast cell cycle mutant (K12) showed that histone mRNA accumulates periodically during the cell cycle and reaches its highest level in the S phase. The direct correlation between the initiation of DNA synthesis and the accumulation of histone mRNA to high levels in S phase demonstrated the strict interdependence of these two events. Moreover, a critical period necessary for histone mRNA accumulation occurred late in G1 phase. If cells were incubated at the nonpermissive temperature during this critical period, the amount of histone mRNA remained at the basal level. Transcription rate measurements indicated that the triggering of histone mRNA synthesis occurred in late G1 and this mRNA was synthesized at its maximal rate 3 to 5 h before its peak of accumulation. However, if cells were prohibited from synthesizing DNA as a consequence of the temperature-sensitive block in G1, the synthesis of histone mRNA was not initiated.

Prediction of Key Factor Controlling G1/S Phase in the Mammalian Cell Cycle Using System Analysis

2008 ◽  
Vol 106 (4) ◽  
pp. 368-374 ◽  
Author(s):  
Yoshihiko Tashima ◽  
Hiroyuki Hamada ◽  
Masahiro Okamoto ◽  
Taizo Hanai
Keyword(s):  
Cell Cycle ◽  
Mammalian Cell ◽  
System Analysis ◽  
S Phase ◽  
Mammalian Cell Cycle ◽  
Key Factor

scholarly journals Restriction Point timing and cell cycle variability – a re-evaluation

2020 ◽  
Author(s):  
Robert F. Brooks
Keyword(s):  
Cell Cycle ◽  
Growth Factor ◽  
Mammalian Cell ◽  
Time Lapse ◽  
S Phase ◽  
Critical Transition ◽  
Restriction Point ◽  
Cycle Times ◽  
Mammalian Cell Cycle ◽  
Step Down

AbstractThe Restriction Point (R) in the mammalian cell cycle is regarded as a critical transition in G1 when cells become committed to enter S phase even in the absence of further growth factor stimulation. Classic time-lapse studies by Zetterberg and Larsson suggested that the acquisition of growth factor independence (i.e. passage of R) occurred very abruptly 3-4 hours after mitosis, with most cell cycle variability arising between R and entry into S phase. However, the cycle times of the post-R cells that continued on to mitosis after serum step-down without perturbation were far less variable than the control cells with which they were compared. A re-analysis of the data, presented here, shows that when the timing of R and entry in mitosis are compared for the same experiments, the curves are superimposable and statistically indistinguishable. This indicates that the data are compatible with the timing of R contributing to much of the overall variability in the cell cycle, contrary to the conclusions of Zetterberg and colleagues.

scholarly journals Use of a cell cycle mutant to delineate the critical period for the control of histone mRNA levels in the mammalian cell cycle.

1984 ◽  
Vol 4 (11) ◽  
pp. 2364-2369 ◽  
Author(s):  
A Artishevsky ◽  
A M Delegeane ◽  
A S Lee
Keyword(s):  
Cell Cycle ◽  
Critical Period ◽  
Fibroblast Cell ◽  
Temporal Analysis ◽  
S Phase ◽  
Temperature Sensitive ◽  
Mrna Levels ◽  
Histone Mrna ◽  
Mrna Accumulation ◽  
Mammalian Cell Cycle

Temporal analysis of DNA replication and histone mRNA accumulation in a hamster fibroblast cell cycle mutant (K12) showed that histone mRNA accumulates periodically during the cell cycle and reaches its highest level in the S phase. The direct correlation between the initiation of DNA synthesis and the accumulation of histone mRNA to high levels in S phase demonstrated the strict interdependence of these two events. Moreover, a critical period necessary for histone mRNA accumulation occurred late in G1 phase. If cells were incubated at the nonpermissive temperature during this critical period, the amount of histone mRNA remained at the basal level. Transcription rate measurements indicated that the triggering of histone mRNA synthesis occurred in late G1 and this mRNA was synthesized at its maximal rate 3 to 5 h before its peak of accumulation. However, if cells were prohibited from synthesizing DNA as a consequence of the temperature-sensitive block in G1, the synthesis of histone mRNA was not initiated.

scholarly journals Towards a systems biology approach to mammalian cell cycle: modeling the entrance into S phase of quiescent fibroblasts after serum stimulation

2009 ◽  
Vol 10 (Suppl 12) ◽  
pp. S16 ◽  
Author(s):  
Roberta Alfieri ◽  
Matteo Barberis ◽  
Ferdinando Chiaradonna ◽  
Daniela Gaglio ◽  
Luciano Milanesi ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Systems Biology ◽  
Mammalian Cell ◽  
S Phase ◽  
Serum Stimulation ◽  
Mammalian Cell Cycle
Sign in / Sign up

Export Citation Format

Share Document