scholarly journals Serum from partially hepatectomized rats induces primary hepatocytes to enter S phase: a role for prostaglandins?

1988 ◽  
Vol 91 (4) ◽  
pp. 549-553
Author(s):  
P. Little ◽  
G.G. Skouteris ◽  
M.G. Ord ◽  
L.A. Stocken
Keyword(s):  
Dna Synthesis ◽  
Culture Medium ◽  
Rat Hepatocytes ◽  
S Phase ◽  
Primary Hepatocytes ◽  
Adult Rat ◽  
Prostaglandin Release ◽  
Adult Rat Hepatocytes ◽  
Growth Promoting ◽  
Hepatocyte Growth

Serum obtained from partially hepatectomized rats 1, 3 or 24 h after operation was more effective in stimulating DNA synthesis in primary adult rat hepatocytes than serum from sham-operated rats; exposure to the serum for 2 h was sufficient to promote growth. Serum from the partially hepatectomized rats contained elevated levels of PGE2 and PGF2 alpha; it promoted hepatocytes to release prostaglandins into their culture medium. Growth-promoting effects of the serum and its capacity to elicit prostaglandin release into the culture medium were inhibited by 0.1 mM-indomethacin or 1 mM-aspirin. 0.1 mM-indomethacin also prevented DNA synthesis if the inhibitor were added 4 h after growth had been initiated by serum from partially hepatectomized rats, suggesting that prostaglandins continue to be important for the maintenance of hepatocyte growth for at least 6 h.

Induction and regulation of connexin26 by glucagon in primary cultures of adult rat hepatocytes

1995 ◽  
Vol 108 (8) ◽  
pp. 2771-2780 ◽  
Author(s):  
T. Kojima ◽  
T. Mitaka ◽  
Y. Shibata ◽  
Y. Mochizuki
Keyword(s):  
Culture Medium ◽  
Primary Cultures ◽  
Minor Component ◽  
Rat Hepatocytes ◽  
Primary Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth ◽  
A Minor ◽  
Junction Proteins

In the adult rat hepatocyte, the gap junction proteins consist of a major component, connexin32 (Cx32) and a minor component, connexin26 (Cx26). Although we recently reported our success in inducing and maintaining Cx32 in adult rat hepatocytes cultured in serum-free L-15 medium supplemented with epidermal growth factor and 2% dimethyl sulfoxide, it was very difficult to induce Cx26 in the primary hepatocytes. In the present study, we found that the addition of 10(−7) M glucagon into the culture medium could dramatically induce Cx26 mRNA and protein. Although the expression of Cx32 mRNA was also influenced by glucagon, the increase of the expression was small. Immunocytochemically, Cx26-positive spots were observed between most adjacent cells and were co-localized with the Cx32-positive spots. We also examined whether 0.5 mM dibutyl cyclic AMP could induce expression of Cx26 in the cells. The effect of dexamethasone on the expression of Cx26 mRNA compared to that of Cx32 mRNA was examined. For the induction and maintenance of Cx26 mRNA, more than 10(−7) M dexamethasone was necessary in this culture. These results suggest that expression of Cx26 in hepatocytes may be regulated by the concentrations of glucagon and glucocorticoid hormones.

Effects of intracellular cyclic AMP and cyclic GMP levels on DNA synthesis of young-adult rat hepatocytes in primary culture

1992 ◽  
Vol 200 (2) ◽  
pp. 404-409 ◽  
Author(s):  
Masahiro Miyazaki ◽  
Syarifuddin Wahid ◽  
Liyan Bai ◽  
Masayoshi Namba
Keyword(s):  
Young Adult ◽  
Cyclic Amp ◽  
Dna Synthesis ◽  
Primary Culture ◽  
Cyclic Gmp ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

DNA synthesis in cultured adult rat hepatocytes: effect of serum and calcium

2008 ◽  
Vol 9 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Tor-Erik Sand ◽  
Ivar Prydz Gladhaug ◽  
Magne Refsnes ◽  
Thoralf Christoffersen
Keyword(s):  
Dna Synthesis ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

scholarly journals Hormonal stimulation of DNA synthesis in primary cultures of adult rat hepatocytes.

1976 ◽  
Vol 73 (10) ◽  
pp. 3589-3593 ◽  
Author(s):  
R. A. Richman ◽  
T. H. Claus ◽  
S. J. Pilkis ◽  
D. L. Friedman
Keyword(s):  
Dna Synthesis ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Hormonal Stimulation ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Stimulation Of

Stimulation of DNA synthesis in primary cultures of adult rat hepatocytes by rat platelet-associated substance(s)

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Alastair J. Strain ◽  
Joan A. McGowan ◽  
Nancy L. R. Bucher
Keyword(s):  
Dna Synthesis ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Stimulation Of
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