DNA synthesis in cultured adult rat hepatocytes: effect of serum and calcium

2008 ◽  
Vol 9 (1) ◽  
pp. 20-26 ◽  
Author(s):  
Tor-Erik Sand ◽  
Ivar Prydz Gladhaug ◽  
Magne Refsnes ◽  
Thoralf Christoffersen
Keyword(s):  
Dna Synthesis ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes

scholarly journals Serum from partially hepatectomized rats induces primary hepatocytes to enter S phase: a role for prostaglandins?

1988 ◽  
Vol 91 (4) ◽  
pp. 549-553
Author(s):  
P. Little ◽  
G.G. Skouteris ◽  
M.G. Ord ◽  
L.A. Stocken
Keyword(s):  
Dna Synthesis ◽  
Culture Medium ◽  
Rat Hepatocytes ◽  
S Phase ◽  
Primary Hepatocytes ◽  
Adult Rat ◽  
Prostaglandin Release ◽  
Adult Rat Hepatocytes ◽  
Growth Promoting ◽  
Hepatocyte Growth

Serum obtained from partially hepatectomized rats 1, 3 or 24 h after operation was more effective in stimulating DNA synthesis in primary adult rat hepatocytes than serum from sham-operated rats; exposure to the serum for 2 h was sufficient to promote growth. Serum from the partially hepatectomized rats contained elevated levels of PGE2 and PGF2 alpha; it promoted hepatocytes to release prostaglandins into their culture medium. Growth-promoting effects of the serum and its capacity to elicit prostaglandin release into the culture medium were inhibited by 0.1 mM-indomethacin or 1 mM-aspirin. 0.1 mM-indomethacin also prevented DNA synthesis if the inhibitor were added 4 h after growth had been initiated by serum from partially hepatectomized rats, suggesting that prostaglandins continue to be important for the maintenance of hepatocyte growth for at least 6 h.

Stimulation of DNA synthesis in primary cultures of adult rat hepatocytes by rat platelet-associated substance(s)

In Vitro ◽  
1982 ◽  
Vol 18 (2) ◽  
pp. 108-116 ◽  
Author(s):  
Alastair J. Strain ◽  
Joan A. McGowan ◽  
Nancy L. R. Bucher
Keyword(s):  
Dna Synthesis ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Stimulation Of
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