scholarly journals Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localization and involvement in parasite-host cell interaction

1990 ◽  
Vol 96 (3) ◽  
pp. 485-490
Author(s):  
T. Souto-Padron ◽  
O.E. Campetella ◽  
J.J. Cazzulo ◽  
W. de Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cysteine Proteinase ◽  
Cell Interaction ◽  
Immunocytochemical Localization ◽  
Flagellar Pocket ◽  
Surface Localization ◽  
Axenic Cultures ◽  
Cytoplasmic Structures ◽  
Host Cell Interaction

A monospecific polyclonal antibody obtained against a cysteine proteinase isolated from epimastigotes of Trypanosoma cruzi was used for the immunocytochemical localization of the protein by electron microscopy and to analyse the role played by cysteine proteinase in the process of T. cruzi-host cell interaction. Cytoplasmic structures that correspond to elements of the endosomal-lysosomal (reservosome) system found in epimastigote, amastigote and trypomastigote forms reacted intensely with colloidal gold-labelled antibodies using on-section indirect labelling. The surface of most of the tissue culture-derived trypomastigotes was not labelled. However, the flagellar pocket of this form was labelled. All epimastigotes obtained from axenic cultures and amastigote-like forms found in the supernatant of vertebrate cells heavily infected with T. cruzi had their surface intensely labelled, indicating also the surface localization of the protein. Incubation of the parasites in the presence of a sub-agglutinating concentration of the anti-cysteine proteinase antibody led to a marked increase in their uptake by macrophages. In contrast, addition of the F(ab')2 portion of the same antibody significantly reduced the uptake of the parasites by the macrophages. The results obtained strongly suggest an important participation of cysteine proteinase in the process of T. cruzi-macrophage interaction.

scholarly journals Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro

1993 ◽  
Vol 88 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Mauricio R. M. P. Luz ◽  
Maria de Nazaré C. Soeiro ◽  
Tania C. Araújo-Jorge
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Interaction ◽  
Host Cell Interaction

Ecto-ATPase activity on the surface of Trypanosoma cruzi and its possible role in the parasite?host cell interaction

2003 ◽  
Vol 91 (4) ◽  
pp. 273-282 ◽  
Author(s):  
Danielle F. R. Bisaggio ◽  
Carlos Eduardo Peres-Sampaio ◽  
Jos� Roberto Meyer-Fernandes ◽  
Tha�s Souto-Padr�n
Keyword(s):  
Trypanosoma Cruzi ◽  
Atpase Activity ◽  
Host Cell ◽  
Cell Interaction ◽  
Host Cell Interaction

scholarly journals Revealing Annexin A2 and ARF-6 enrollment during Trypanosoma cruzi extracellular amastigote-host cell interaction

2015 ◽  
Vol 8 (1) ◽  
Author(s):  
Thaise Lara Teixeira ◽  
Lilian Cruz ◽  
Renato Arruda Mortara ◽  
Claudio Vieira Da Silva
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Interaction ◽  
Annexin A2 ◽  
Host Cell Interaction

scholarly journals Review onTrypanosoma cruzi: Host Cell Interaction

2010 ◽  
Vol 2010 ◽  
pp. 1-18 ◽  
Author(s):  
Wanderley de Souza ◽  
Tecia Maria Ulisses de Carvalho ◽  
Emile Santos Barrias
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Mammalian Cells ◽  
Cell Types ◽  
Cell Interaction ◽  
Host Cells ◽  
Mammalian Host ◽  
Blood Sucking ◽  
Number Of Individuals ◽  
Host Cell Interaction

Trypanosoma cruzi, the causative agent of Chagas' disease, which affects a large number of individuals in Central and South America, is transmitted to vertebrate hosts by blood-sucking insects. This protozoan is an obligate intracellular parasite. The infective forms of the parasite are metacyclic and bloodstream trypomastigote and amastigote. Metacyclic trypomastigotes are released with the feces of the insect while amastigotes and bloodstream trypomastigotes are released from the infected host cells of the vertebrate host after a complex intracellular life cycle. The recognition between parasite and mammalian host cell involves numerous molecules present in both cell types. Here, we present a brief review of the interaction betweenTrypanosoma cruziand its host cells, mainly emphasizing the mechanisms and molecules that participate in theT. cruziinvasion process of the mammalian cells.

Bacteriophages Associated with Turkey Coecums in Bluecomb-Infected and Healthy Birds

1969 ◽  
Vol 27 ◽  
pp. 226-227
Author(s):  
A. E. Ritchie
Keyword(s):  
Host Cell ◽  
Causal Relationship ◽  
Cell Cultures ◽  
Cell Interaction ◽  
Etiologic Agent ◽  
Viral Origin ◽  
Intestinal Contents ◽  
Host Cell Interaction

The cause of bluecomb disease in turkeys is unknown. Filtration of infective intestinal contents suggests a viral origin. To date, it has not been possible to isolate the etiologic agent in various cell cultures. The purpose of this work was to characterize as many virus-like entities as were recognizable in intestines of both healthy and bluecomb-infected turkeys. By a comparison of the viral populations it was hoped that some insight might be gained into the cause of this disease. Studies of turkey hemorraghic enteritis by Gross and Moore (Avian Dis. 11: 296-307, 1967) have suggested that a bacteriophage-host cell interaction may bear some causal relationship to that disease.

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