scholarly journals Improved enzyme-linked immunoadsorbent assay (ELISA) for the study of Trypanosoma cruzi-host cell interaction in vitro

1993 ◽  
Vol 88 (2) ◽  
pp. 235-241 ◽  
Author(s):  
Mauricio R. M. P. Luz ◽  
Maria de Nazaré C. Soeiro ◽  
Tania C. Araújo-Jorge
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cell Interaction ◽  
Host Cell Interaction

scholarly journals Advances in Zika Virus–Host Cell Interaction: Current Knowledge and Future Perspectives

2019 ◽  
Vol 20 (5) ◽  
pp. 1101 ◽  
Author(s):  
Jae Lee ◽  
Ok Shin
Keyword(s):  
Host Cell ◽  
Zika Virus ◽  
Current Knowledge ◽  
Cell Interaction ◽  
Human Pathogens ◽  
Zikv Infection ◽  
In Vivo Models ◽  
Host Cell Interaction

Emerging mosquito-transmitted RNA viruses, such as Zika virus (ZIKV) and Chikungunya represent human pathogens of an immense global health problem. In particular, ZIKV has emerged explosively since 2007 to cause a series of epidemics in the South Pacific and most recently in the Americas. Although typical ZIKV infections are asymptomatic, ZIKV infection during pregnancy is increasingly associated with microcephaly and other fetal developmental abnormalities. In the last few years, genomic and molecular investigations have established a remarkable progress on the pathogenic mechanisms of ZIKV infection using in vitro and in vivo models. Here, we highlight recent advances in ZIKV-host cell interaction studies, including cellular targets of ZIKV, ZIKV-mediated cell death mechanisms, host cell restriction factors that limit ZIKV replication, and immune evasion mechanisms utilized by ZIKV. Understanding of the mechanisms of ZIKV–host interaction at the cellular level will contribute crucial insights into the development of ZIKV therapeutics and vaccines.

scholarly journals Akt Inhibitor MK2206 Prevents Influenza pH1N1 Virus InfectionIn Vitro

2014 ◽  
Vol 58 (7) ◽  
pp. 3689-3696 ◽  
Author(s):  
Oxana V. Denisova ◽  
Sandra Söderholm ◽  
Salla Virtanen ◽  
Carina Von Schantz ◽  
Dmitrii Bychkov ◽  
...  
Keyword(s):  
Virus Infection ◽  
Host Cell ◽  
Treatment Options ◽  
Cell Interaction ◽  
Akt Signaling ◽  
Akt Inhibitor ◽  
Prevention And Treatment ◽  
Host Cell Interaction

ABSTRACTThe influenza pH1N1 virus caused a global flu pandemic in 2009 and continues manifestation as a seasonal virus. Better understanding of the virus-host cell interaction could result in development of better prevention and treatment options. Here we show that the Akt inhibitor MK2206 blocks influenza pH1N1 virus infectionin vitro. In particular, at noncytotoxic concentrations, MK2206 alters Akt signaling and inhibits endocytic uptake of the virus. Interestingly, MK2206 is unable to inhibit H3N2, H7N9, and H5N1 viruses, indicating that pH1N1 evolved specific requirements for efficient infection. Thus, Akt signaling could be exploited further for development of better therapeutics against pH1N1 virus.

scholarly journals Active Components of Commonly Prescribed Medicines Affect Influenza a Virus-Host Cell Interaction: A Pilot Study

2021 ◽  
Author(s):  
Aleksandr Ianevski ◽  
Rouan Yao ◽  
Eva Zusinaite ◽  
Hilde Lysvand ◽  
Valentyn Oksenych ◽  
...  
Keyword(s):  
Host Cell ◽  
Influenza A Virus ◽  
Drug Target ◽  
Influenza A ◽  
Cell Interaction ◽  
Host Responses ◽  
Target Interaction ◽  
Prescribed Medicines ◽  
Host Cell Interaction

Background: Every year, millions of people are hospitalized, and thousands die from influenza A virus (FLUAV) infection. Most cases of hospitalizations and death occur among elderly. Many of these elderly patients are reliant on medical treatment of underlying chronic diseases, such as arthritis, diabetes, and hypertension. We hypothesized that the commonly prescribed medicines for treatment of underlying chronic diseases can affect host responses to FLUAV infection, and thus contribute to morbidity and mortality associated with influenza. Therefore, the aim of this study was to examine whether commonly prescribed medicines could affect host responses to virus infection in vitro. Methods: We first identified 45 active compounds of medicines commonly prescribed in Central Norway. Then we constructed a drug-target interaction network and identified potential implication of these interactions for FLUAV-host cell interplay. Finally, we tested the effect of 45 drugs on viability, transcription and metabolism of mock- and A/WSN/33(H1N1)-infected human retinal pigment epithelial (RPE) cells. Results: In silico drug-target interaction analysis revealed that many drugs, such as acetylsalicylic acid, atorvastatin, candesartan, and hydroxocobalam, could target and modulate FLUAV-host cell interaction. In vitro experiments showed that these and other compounds at non-cytotoxic concentrations differently affected transcription and metabolism of mock- and FLUAV-infected cells. Conclusion: Many commonly prescribed drugs modulate FLUAV-host cell interactions in vitro and therefore could affect their interplay in vivo, thus, contributing to morbidity and mortality of patients with influenza virus infections.

scholarly journals Active components of commonly prescribed medicines affect influenza A virus-host cell interaction: a pilot study

2021 ◽  
Author(s):  
Aleksandr Ianevski ◽  
Rouan Yao ◽  
Eva Zusinaite ◽  
Hilde Lysvand ◽  
Valentyn Oksenych ◽  
...  
Keyword(s):  
Host Cell ◽  
Influenza A Virus ◽  
Drug Target ◽  
Influenza A ◽  
Cell Interaction ◽  
Host Responses ◽  
Target Interaction ◽  
Prescribed Medicines ◽  
Host Cell Interaction

Background: Every year, millions of people are hospitalized, and thousands die from influenza A virus (FLUAV) infection. Most cases of hospitalizations and death occur among elderly. Many of these elderly patients are reliant on medical treatment of underlying chronic diseases, such as arthritis, diabetes, and hypertension. We hypothesized that the commonly prescribed medicines for treatment of underlying chronic diseases can affect host responses to FLUAV infection, and thus contribute to morbidity and mortality associated with influenza. Therefore, the aim of this study was to examine whether commonly prescribed medicines could affect host responses to virus infection in vitro. Methods: We first identified 45 active compounds of commonly prescribed medicines. Then we constructed a drug-target interaction network and identified potential implication of these interactions for FLUAV-host cell interplay. Finally, we tested the effect of 45 drugs on viability, transcription and metabolism of mock- and FLUAV-infected human retinal pigment epithelial (RPE) cells. Results: In silico drug-target interaction analysis revealed that many drugs, such as atorvastatin, candesartan, and hydroxocobalam, could target and modulate FLUAV-host cell interaction. In vitro experiments showed that these and other compounds at non-cytotoxic concentrations differently affected transcription and metabolism of mock- and FLUAV-infected cells. Conclusion: Many commonly prescribed drugs modulate FLUAV-host cell interactions in silico and in vitro and, therefore, could affect their interplay in vivo, thus, contributing to morbidity and mortality of patients with influenza virus infections.

scholarly journals Cysteine proteinase in Trypanosoma cruzi: immunocytochemical localization and involvement in parasite-host cell interaction

1990 ◽  
Vol 96 (3) ◽  
pp. 485-490
Author(s):  
T. Souto-Padron ◽  
O.E. Campetella ◽  
J.J. Cazzulo ◽  
W. de Souza
Keyword(s):  
Trypanosoma Cruzi ◽  
Host Cell ◽  
Cysteine Proteinase ◽  
Cell Interaction ◽  
Immunocytochemical Localization ◽  
Flagellar Pocket ◽  
Surface Localization ◽  
Axenic Cultures ◽  
Cytoplasmic Structures ◽  
Host Cell Interaction

A monospecific polyclonal antibody obtained against a cysteine proteinase isolated from epimastigotes of Trypanosoma cruzi was used for the immunocytochemical localization of the protein by electron microscopy and to analyse the role played by cysteine proteinase in the process of T. cruzi-host cell interaction. Cytoplasmic structures that correspond to elements of the endosomal-lysosomal (reservosome) system found in epimastigote, amastigote and trypomastigote forms reacted intensely with colloidal gold-labelled antibodies using on-section indirect labelling. The surface of most of the tissue culture-derived trypomastigotes was not labelled. However, the flagellar pocket of this form was labelled. All epimastigotes obtained from axenic cultures and amastigote-like forms found in the supernatant of vertebrate cells heavily infected with T. cruzi had their surface intensely labelled, indicating also the surface localization of the protein. Incubation of the parasites in the presence of a sub-agglutinating concentration of the anti-cysteine proteinase antibody led to a marked increase in their uptake by macrophages. In contrast, addition of the F(ab')2 portion of the same antibody significantly reduced the uptake of the parasites by the macrophages. The results obtained strongly suggest an important participation of cysteine proteinase in the process of T. cruzi-macrophage interaction.

Ecto-ATPase activity on the surface of Trypanosoma cruzi and its possible role in the parasite?host cell interaction

2003 ◽  
Vol 91 (4) ◽  
pp. 273-282 ◽  
Author(s):  
Danielle F. R. Bisaggio ◽  
Carlos Eduardo Peres-Sampaio ◽  
Jos� Roberto Meyer-Fernandes ◽  
Tha�s Souto-Padr�n
Keyword(s):  
Trypanosoma Cruzi ◽  
Atpase Activity ◽  
Host Cell ◽  
Cell Interaction ◽  
Host Cell Interaction
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