A Cytological and Histochemical Study of the Stria Vascularis of the Guinea-pig's Ear

1961 ◽  
Vol s3-102 (57) ◽  
pp. 75-82
Author(s):  
JOHN T. Y. CHOU

The cells of the stria vascularis of the guinea-pig have been studied morphologically and histochemically. The stria vascularis consists of three layers of cells with bloodcapillaries. Apart from some yellowish pigments, the only cytoplasmic inclusions seen in the living cells and in the fixed materials are numerous rod-shaped mitochondria and spherical lipid globules. The mitochondria are about 1.5 µ in length with a diameter of 0.8 µ they contain phospholipid. The lipid globules are about 1.0 µ to 1.5 µ in diameter; they contain phospholipid and a small amount of cerebroside. The cytoplasm of these cells contains a small amount of diffused phospholipid and cerebroside.

1981 ◽  
Vol 92 (1-6) ◽  
pp. 433-437 ◽  
Author(s):  
F. Aporti ◽  
L. Facci ◽  
A. Pastorello ◽  
R. Siliprandi ◽  
M. Savastano ◽  
...  

1990 ◽  
Vol 49 (Suppl-6) ◽  
pp. 40-46
Author(s):  
Shoji Nishiyama ◽  
Harumichi Seguchi ◽  
Eva Garcia

1955 ◽  
Vol s3-96 (33) ◽  
pp. 49-56
Author(s):  
W. G. BRUCE CASSELMAN ◽  
JOHN R. BAKER

1. The neurones studied were those of the anterior mesenteric and coeliac ganglia of immature rabbits. 2. Ectoplasm and endoplasm can be distinguished in these cells. 3. Two kinds of cytoplasmic inclusions occur. These are (a) spherical or spheroid lipid globules or lipochondria, which are confined to the endoplasm; and (b) very minute threads and granules, regarded as mitochondria. 4. A wide variety of histochemical tests was used. The results indicate that the lipochondria consist of galactolipid (cerebrosid) and phospholipid, though the smallest ones may perhaps consist of galactolipid only. 5. The objects regarded as mitochondria are too small for accurate investigation by in situ histochemical methods, but they give positive reactions for phospholipid and protein.


1978 ◽  
Vol 88 (11) ◽  
pp. 1825???1835 ◽  
Author(s):  
DANIEL C. MARCUS ◽  
RUEDIGER THALMANN ◽  
NANCY Y. MARCUS

1965 ◽  
Vol 67 (5) ◽  
pp. 600-619 ◽  
Author(s):  
E. L. Rodriguez Echandia ◽  
Mario H. Burgos

1991 ◽  
Vol 111 (6) ◽  
pp. 1041-1050 ◽  
Author(s):  
Shoji Nishiyama ◽  
Eva Garcia Del Saz ◽  
Harumichi Seguchi
Keyword(s):  

1981 ◽  
Vol 178 (3) ◽  
pp. 237-240 ◽  
Author(s):  
Chr Kittas ◽  
L. Henry ◽  
C. Papadimitriou ◽  
N. X. Papacharalampous

If a guinea-pig R is sensitized by the transplantation of a skin homograft from a guinea-pig D , the intradermal injection into R of antigenic matter from D provokes an inflammatory response of delayed onset ( direct reaction ) that is outwardly and histologically similar to a tuberculin reaction. A reaction of the same kind is provoked when living cells expressed from the regional lymph nodes of R are injected intradermally into D ( transfer reaction ). The transfer reaction is interpreted as a local passive transfer of the state of reactivity dis­closed by the direct reaction. When due allowance is made for the sharing of antigens by members of outbred populations of guinea-pigs, both direct and transfer reactions are immunologically specific (§§ 2.3, 2.4, 3.1). The direct reaction can be provoked by cellular extracts (§ 2.3) as well as by living cells (§ 2-4). Its intensity is strongly correlated with the strength of the homograft reaction (§ 8). After sensitization by a single set of skin homografts, direct reactivity persists for a period of the order of hundreds of days (§ 4). ‘Hyperimmunization’ by the repeated injection of D cells into R is accompanied by a decline of direct reactivity (§ 6). The transfer reaction can be mediated through blood leucocytes (§ 3.2) and peritoneal exudate cells (§ 3.3) as well as by cells from lymph nodes. Although the regional node is the first node to be activated by a regional homograft, activity spreads thereafter to other nodes (§ 3.4). After the intravenous injection into R of 40 million lymphoid cells from D , transfer reactivity can be demonstrated in the leucocytes of R within 3 days (§ 3.2). Sensitized cells killed by heating, freezing or drying do not mediate the transfer reaction (§ 7.1). Extracts of leucocytes disrupted in the presence of deoxyribonuclease (Lawrence’s ‘transfer factor’) cannot deputize for living cells in the transfer reaction (§ 7.3), and evidence that such extracts can transfer direct reactivity to normal guinea-pigs is equivocal (§ 7.4). Disrupted leucocytes and especially blood platelets of syngeneic (‘isologous’) origin can give rise to violent non-specific delayed inflammatory reactions which resemble tuberculin reactions superficially but differ from them histologically (§ 7.3). Direct and transfer reactions were combined in an experiment in which cells or antigenic extracts from D were mixed with sensitized cells from R and then injected into a normal guinea-pig syngeneic with R (§ 3.5). Thus both direct and transfer reactions seem to depend essentially upon the local engagement of antigen with sensitized cells. Neither direct nor transfer reactions were affected by the administration of high doses of hydrocortisone (§ 5). It has not been possible to demonstrate direct or transfer reactions in mice, but the transfer reaction in rabbits is violent and prolonged (§ 3.0). (Other workers have demon­ strated direct reactivity in man.) The evidence, taken in the round, suggests that the delayed cutaneous inflammatory reactions revealed by the direct and transfer tests are manifestations of the homograft reaction, and not of an immunological reaction associated with some different iso-antigenic system. It thus supports the analogy long since drawn between the homograft and tuberculin reactions, and upholds the contention that the ‘second set’ homograft reaction reveals a pre-existing and not are-awakened (anamnestic) sensitivity (§ 8).


1981 ◽  
Vol 8 (2) ◽  
pp. 45-54
Author(s):  
Fumihisa Hiraide ◽  
Tetsuzo Inouye ◽  
Masamichi Sawada ◽  
Norimasa Miyakogawa ◽  
Yasukiyo Tsubaki ◽  
...  

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