The use of methyl green as a histochemical reagent

1965 ◽  
Vol s3-106 (73) ◽  
pp. 3-13
Author(s):  
JOHN R. BAKER ◽  
ELIZABETH G. M. WILLIAMS
Keyword(s):  
Connective Tissue ◽  
Malachite Green ◽  
Helix Aspersa ◽  
Common Snail ◽  
Methyl Green ◽  
Dilute Solution ◽  
Dilute Solutions ◽  
The Common ◽  
Snail Helix Aspersa

The cation of methyl green carriea two poaitive charges, that of malachite green only one; but the two dyes behave towards tissue-constituents in almost exactly the same way. These dyes are not specific for chromatin. They colour certain objects that are devoid of DNA, even when they are used in very dilute solution. The granules of cells called Körnchenzellen in the connective tissue of the common snail, Helix aspersa, are strongly coloured by both dyes from very dilute solutions, and thus provide a striking instance of the unspecificity of these dyes. Malachite green, which is stable and free from contamination by metachromstic impurities, can advantageously replace the methyl green commonly used in mixtures with pyronine. It is suggested that pyronine may have a greater capacity for penetrating into close-textured objects, such ss nucleoli and ribosomes, than methyl and malachite greens.

Peptides in the brain of the common snail, Helix aspersa

1982 ◽  
Vol 10 (5) ◽  
pp. 384-385 ◽  
Author(s):  
MARION E. WILLIAMSON ◽  
PIERS C. EMSON
Keyword(s):  
Helix Aspersa ◽  
Common Snail ◽  
The Common ◽  
Snail Helix Aspersa ◽  
The Brain

scholarly journals The composition of intracellular granules from the metal-accumulating cells of the common garden snail (Helix aspersa)

1981 ◽  
Vol 194 (2) ◽  
pp. 507-511 ◽  
Author(s):  
B Howard ◽  
P C H Mitchell ◽  
A Ritchie ◽  
K Simkiss ◽  
M Taylor
Keyword(s):  
Metal Ion ◽  
Heavy Metal Ions ◽  
Inorganic Material ◽  
Common Garden ◽  
Helix Aspersa ◽  
X Ray ◽  
The Common ◽  
Main Components ◽  
Snail Helix Aspersa ◽  
Enzymic Assay

Certain cells in the hepatopancreas of the common garden snail (Helix aspersa) contain intracellular granules that are sites of metal-ion accumulation. These granules have been extracted and investigated by u.v. and i.r. spectroscopy, atomic-absorption spectroscopy, X-ray microanalysis, thermogravimetric analysis, enzymic assay and microanalysis. The deposits contain about 18% (w/w) water, 5% (w/w) organic matter and 76% (w/w) inorganic material of which the main components are Ca2+, Mg2+ and P2O7(4)-. The possible origin of these granules is discussed, as is their role in detoxifying heavy-metal ions.

Outgrowths from Pieces of Helix aspersa, the Common Snail

Nature ◽  
1931 ◽  
Vol 128 (3241) ◽  
pp. 1002-1003 ◽  
Author(s):  
J. BRONTË GATENBY
Keyword(s):  
Helix Aspersa ◽  
Common Snail ◽  
The Common

Two New Cell Types in the Retina of a Snail Helix aspersa

1974 ◽  
Vol 32 ◽  
pp. 284-285
Author(s):  
Jean L. Brandenburger ◽  
Richard M. Eakin
Keyword(s):  
Sensory Cell ◽  
Ganglion Cells ◽  
Common Garden ◽  
Cell Types ◽  
Helix Aspersa ◽  
Spherical Nucleus ◽  
Golgi Bodies ◽  
The Common ◽  
Snail Helix Aspersa ◽  
Electron Lucent

Previous studies have described the retina of the common garden snail Helix aspersa to be composed of two cell types: sensory and pigmented. We have now found a third, a ganglion cell, and another kind of sensory cell, both heretofore undescribed.Ganglion cells (Fig. 1) are large (11-16 fan in diameter), ovoid, and few in number. We estimate about 12 per eye. They occur only along the periphery of the retina. Each possesses a large spherical nucleus centrally situated in an electron lucent cytoplasm which contains numerous organelles and in clusions: mitochondria, Golgi bodies, EH, lysosomes, glycogen granules, microtubules, ribosomes, fine filaments and clusters of irregular vesicles. These vesicles (insert, Fig. 1) vary in size (500 A to 1200 A in diameter) and density (clear, granular or dense-cored).

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