scholarly journals Activation of Extracellular-Signal Regulated Kinase by Epidermal Growth Factor Is Potentiated by cAMP-Elevating Agents in Primary Cultures of Adult Rat Hepatocytes

2011 ◽  
Vol 34 (10) ◽  
pp. 1542-1552 ◽  
Author(s):  
Hajime Moteki ◽  
Mitsutoshi Kimura ◽  
Masahiko Ogihara
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Extracellular Signal Regulated Kinase ◽  
Adult Rat ◽  
Extracellular Signal ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth

Quantitative aspects of the effects of insulin, epidermal growth factor and dexamethasone on DNA synthesis in cultured adult rat hepatocytes

1985 ◽  
Vol 109 (3) ◽  
pp. 369-377 ◽  
Author(s):  
Tor-Erik Sand ◽  
Gunnar Brønstad ◽  
Vemund Digernes ◽  
Anne Killi ◽  
Wasfiyeh Amara ◽  
...  
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Dna Synthesis ◽  
Time Course ◽  
Primary Cultures ◽  
Rat Hepatocytes ◽  
Maximal Effect ◽  
Adult Rat ◽  
Adult Rat Hepatocytes ◽  
Epidermal Growth

Abstract. Epidermal growth factor (EGF) and insulin in combination have previously been shown to initiate S-phase in primary cultures of adult rat hepatocytes. We here describe the detailed time course and dosedependency of the effects of EGF and insulin on DNA synthesis in cultured hepatocytes. The DNA synthesis was assessed either biochemically or autoradiographically with a fairly good correlation between the two methods. DNA synthesis started 24–30 h after plating of the cells and peaked at approximately 70 h. Up to 70% of the cells entered DNA synthesis during this period. EGF and insulin acted synergistically on the DNA synthesis. Dexamethasone raised the DNA synthesis slightly, maximal effect occurred at concentrations above 2.5 nm and this agent was routinely used in the experiments with EGF and insulin. In the presence of 0.4 μm insulin from the time of plating, EGF dose-dependently increased the DNA synthesis with maximal effect at 5–15 nm. When added in combination with 1.7 nm EGF, insulin enhanced the DNA synthesis over the concentration range from 0.1 to 3 nm. These studies show that primary cultures of hepatocytes are useful in assessing the quantitative aspects of the interactions between the growth stimulating effects of hormones.

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