Development of Solid-Phase Radical Reactions Using Oxime Ethers as a Radical Acceptor

2000 ◽  
Vol 120 (8) ◽  
pp. 667-676 ◽  
Author(s):  
Hideto MIYABE
1999 ◽  
Vol 40 (40) ◽  
pp. 7285-7288 ◽  
Author(s):  
Stephen Caddick ◽  
Daniel Hamza ◽  
Sjoerd N Wadman

2002 ◽  
Vol 4 (10) ◽  
pp. 1775-1777 ◽  
Author(s):  
Stephen Caddick ◽  
Daniel Hamza ◽  
Sjoerd N. Wadman ◽  
Jonathan D. Wilden

2000 ◽  
Vol 2 (10) ◽  
pp. 1443-1445 ◽  
Author(s):  
Hideto Miyabe ◽  
Chihiro Konishi ◽  
Takeaki Naito

Author(s):  
K. Pegg-Feige ◽  
F. W. Doane

Immunoelectron microscopy (IEM) applied to rapid virus diagnosis offers a more sensitive detection method than direct electron microscopy (DEM), and can also be used to serotype viruses. One of several IEM techniques is that introduced by Derrick in 1972, in which antiviral antibody is attached to the support film of an EM specimen grid. Originally developed for plant viruses, it has recently been applied to several animal viruses, especially rotaviruses. We have investigated the use of this solid phase IEM technique (SPIEM) in detecting and identifying enteroviruses (in the form of crude cell culture isolates), and have compared it with a modified “SPIEM-SPA” method in which grids are coated with protein A from Staphylococcus aureus prior to exposure to antiserum.


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