scholarly journals BIOCHEMICAL STUDIES ON MECHANISM OF THE ACTION OF CARDIAC GLYCOSIDES

1960 ◽  
Vol 9 (1) ◽  
pp. 83-90 ◽  
Author(s):  
KAZUO KAWAI
Author(s):  
M. C. Buhrer ◽  
R. A. Mathews

Ruthenium red has been used as a stain to demonstrate a variety of extracellular materials, especially acid mucopolysaccharides. It also reacts with certain intracellular and extracellular lipids. Since biochemical studies in our laboratory demonstrated the presence of a variety of monosaccharides in human hair ruthenium red staining procedures were adopted in order to evaluate the presence and morphological location of acid oligosaccharides in the keratinized aspect of hair.


Author(s):  
Robert R. Cardell

Hypophysectomy of the rat renders this animal deficient in the hormones of the anterior pituitary gland, thus causing many primary and secondary hormonal effects on basic liver functions. Biochemical studies of these alterations in the rat liver cell are quite extensive; however, relatively few morphological observations on such cells have been recorded. Because the available biochemical information was derived mostly from disrupted and fractionated liver cells, it seemed desirable to examine the problem with the techniques of electron microscopy in order to see what changes are apparent in the intact liver cell after hypophysectomy. Accordingly, liver cells from rats which had been hypophysectomized 5-120 days before sacrifice were studied. Sham-operated rats served as controls and both hypophysectomized and control rats were fasted 15 hours before sacrifice.


1999 ◽  
Vol 38 (05) ◽  
pp. 164-168 ◽  
Author(s):  
Gloria Ruiz Hernandez ◽  
C. Sanchez Marchori ◽  
J. Munoz Moliner ◽  
C. Martinez Carsi

SummaryA 26-year-old man with a previous history of external twin bursitis was remitted to our Department for a bone scintigraphy. Before the study, the patient performed an elevated number of intense sprints. Bone scintigraphy showed a bilaterally increased activity in both anterior rectum muscles suggesting rhabdomyolysis. Biochemical studies and MRT confirmed the diagnosis.


2020 ◽  
pp. 15-21
Author(s):  
Arindam Ghosh ◽  
Soumya Majumder ◽  
Sumedha Saha ◽  
Malay Bhattacharya

Beneficial properties of shade trees of tea plantations other than their medicinal properties have been extensively studied. This research was initiated to explore the properties of some shade trees with special emphasis on their antioxidant and antibacterial properties. Leaves from shade tree like Dalbergia sissoo (DS), Cassia siamea (CS), Derris robusta (DR), Leucaena leucocephala (LL), Acacia lenticularis (AL) and Melia azedarach (MA) were used for the study. Characterization of shade tree leaves by determination of moisture, crude fibre and ash content and tests of non polar – polar solvent extracts for steroid, tannins, cardiac glycosides and coumarin, free radical scavenging, ferric reducing power, NO scavenging activities, quantification of Flavonoids and antibacterial activity were conducted. The average moisture, crude fibre and ash percentage of shade tree plants were found to be 62.95, 11.28 and 1.86 respectively. Methanol, ethanol, acetone and ethyl acetate respectively proved to be the most potent solvent for various phytochemical extractions as it gave positive results for tests like tannin, steroid, cardiac glycosides and coumarin. AL (91.46%), DR (92.69%), LL (94.32%) and MA (93.34%) leaf extracts showed a high level of DPPH scavenging activity in their water extracts. In DS (88.11%) and CS (83.23%) maximum DPPH scavenging activity was observed in Diethyl ether and Methanol extracts respectively.  Acetone extracts were more active than the water extracts in exhibiting ferric reducing power and NO scavenging activity. Summation of the quantity revealed that DS showed maximum presence of flavonoids and acetone as most potential for isolation of flavonoids. The decreasing order of summative antibacterial activity was recorded in DS, followed by CS, DR, AL, MA and LL. Chloroform showed the highest summative inhibition zone followed by ethanol, ethyl acetate, diethyl ether, acetone, water,  hexane, benzene and methanol. The antioxidant and antibacterial potential of shade trees were established.


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