scholarly journals Divergence and heterogeneity of the histone gene repeating units in the Drosophila melanogaster species subgroup

2003 ◽  
Vol 78 (5) ◽  
pp. 383-389 ◽  
Author(s):  
Mitsuru Kakita ◽  
Tsunenori Shimizu ◽  
Masami Emoto ◽  
Mariya Nagai ◽  
Miyo Takeguchi ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Histone Gene ◽  
Drosophila Melanogaster Species Subgroup

scholarly journals Correction: Evolution of Eye Morphology and Rhodopsin Expression in the Drosophila melanogaster Species Subgroup

PLoS ONE ◽  
2012 ◽  
Vol 7 (7) ◽  
Author(s):  
Nico Posnien ◽  
Corinna Hopfen ◽  
Maarten Hilbrant ◽  
Margarita Ramos-Womack ◽  
Sophie Murat ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Eye Morphology ◽  
Drosophila Melanogaster Species Subgroup

scholarly journals DNA replication of histone gene repeats in Drosophila melanogaster tissue culture cells: multiple initiation sites and replication pause sites.

1993 ◽  
Vol 13 (7) ◽  
pp. 4098-4106 ◽  
Author(s):  
T Shinomiya ◽  
S Ina
Keyword(s):  
Tissue Culture ◽  
Drosophila Melanogaster ◽  
Dna Replication ◽  
Present Report ◽  
Polymerase Chain Reaction Method ◽  
Mapping Method ◽  
Histone Gene ◽  
Culture Cells ◽  
Tissue Culture Cells ◽  
Replication Intermediates

We showed previously that DNA replication initiates at multiple sites in the 5-kb histone gene repeating unit in early embryos of Drosophila melanogaster. The present report shows evidence that replication in the same chromosomal region initiates at multiple sites in tissue culture cells as well. First, we analyzed replication intermediates by the two-dimensional gel electrophoretic replicon mapping method and detected bubble-form replication intermediates for all fragments restricted at different sites in the repeating unit. Second, we analyzed bromodeoxyuridine-labeled nascent strands amplified by the polymerase chain reaction method and detected little differences in the size distribution of nascent strands specific to six short segments located at different sites in the repeating unit. These results strongly suggest that DNA replication initiates at multiple sites located within the repeating unit. We also found several replication pause sites located at 5' upstream regions of some histone genes.

scholarly journals Pure species discriminate against hybrids in the Drosophila melanogaster species subgroup

2020 ◽  
Author(s):  
Antonio Serrato-Capuchina ◽  
Timothy D. Schwochert ◽  
Stephania Zhang ◽  
Baylee Roy ◽  
David Peede ◽  
...  
Keyword(s):  
Drosophila Melanogaster ◽  
Gene Flow ◽  
Parental Species ◽  
Cuticular Hydrocarbon ◽  
F1 Hybrids ◽  
Sexual Attractiveness ◽  
Pure Species ◽  
Species Pairs ◽  
Fertile Hybrids ◽  
Drosophila Melanogaster Species Subgroup

ABSTRACTIntrogression, the exchange of alleles between species, is a common event in nature. This transfer of alleles between species must happen through fertile hybrids. Characterizing the traits that cause defects in hybrids illuminate how and when gene flow is expected to occur. Inviability and sterility are extreme examples of fitness reductions but are not the only type of defects in hybrids. Some traits specific to hybrids are more subtle but are important to determine their fitness. In this report, we study whether F1 hybrids between two species pairs of Drosophila are as attractive as the parental species. We find that in both species pairs, the sexual attractiveness of the F1 hybrids is reduced and that pure species discriminate strongly against them. We also find that the cuticular hydrocarbon (CHC) profile of the hybrids is intermediate between the parental species. Perfuming experiments show that modifying the CHC profile of the hybrids to resemble pure species improves their chances of mating. Our results show that behavioral discrimination against hybrids might be an important component of the persistence of species that can hybridize.

scholarly journals Molecular evolution of the duplicated Amy locus in the Drosophila melanogaster species subgroup: concerted evolution only in the coding region and an excess of nonsynonymous substitutions in speciation.

Genetics ◽  
1995 ◽  
Vol 141 (1) ◽  
pp. 223-236
Author(s):  
H Shibata ◽  
T Yamazaki
Keyword(s):  
Drosophila Melanogaster ◽  
Concerted Evolution ◽  
Sequence Data ◽  
Coding Region ◽  
Nucleotide Sequence Data ◽  
Nonsynonymous Substitutions ◽  
Specialist Species ◽  
Duplicated Loci ◽  
Flanking Regions ◽  
Drosophila Melanogaster Species Subgroup

Abstract From the analysis of restriction maps of the Amy region in eight sibling species belonging to the Drosophila melanogaster species subgroup, we herein show that the patterns of duplication of the Amy gene are almost the same in all species. This indicates that duplication occurred before speciation within this species subgroup. From the nucleotide sequence data, we show a strong within-species similarity between the duplicated loci in the Amy coding region. This is in contrast to a strong similarity in the 5' and 3' flanking regions within each locus (proximal or distal) throughout the species subgroup. This means that concerted evolution occurred only in the Amy coding region and that differentiated evolution between the duplication occurred in the flanking regions. Moreover, when comparing the species, we also found a significant excess of nonsynonymous substitutions. In particular, all the fixed substitutions specific to D. erecta were found to be nonsynonymous. We thus conclude that adaptive protein evolution occurred in the lineage of D. erecta that is a "specialist" species for host plants and probably also occurs in the process of speciation in general.

Sign in / Sign up

Export Citation Format

Share Document