scholarly journals Composition of eukaryotic gene loci regarding gene conversion units and the presence or the absence of intralocus reciprocal recombination.

1989 ◽  
Vol 64 (4) ◽  
pp. 295-313
Author(s):  
Yoshiaki KITANI
Keyword(s):  
Gene Conversion ◽  
Reciprocal Recombination ◽  
Eukaryotic Gene

scholarly journals Extrachromosomal and chromosomal gene conversion in mammalian cells.

1986 ◽  
Vol 6 (5) ◽  
pp. 1608-1614 ◽  
Author(s):  
J Rubnitz ◽  
S Subramani
Keyword(s):  
Gene Conversion ◽  
Mammalian Cells ◽  
Insertion Mutation ◽  
Chromosomal Gene ◽  
Sequence Organization ◽  
Reciprocal Recombination ◽  
Homologous Fragment ◽  
Double Recombination ◽  
Neomycin Resistance ◽  
Chromosomal Recombination

We constructed substrates to study gene conversion in mammalian cells specifically without the complication of reciprocal recombination events. These substrates contain both an insertion mutation of the neomycin resistance gene (neoX) and an internal, homologous fragment of the neo gene (neo-526), such that gene conversion from neo-526 to neoX restores a functional neo gene. Although two reciprocal recombination events can also produce an intact neo gene, these double recombination events occur much less frequently that gene conversion in mammalian cells, We used our substrates to characterize extrachromosomal gene conversion in recombination-deficient bacteria and in monkey COS cells. Chromosomal recombination was also studied after stable integration of these substrates into the genome of mouse 3T6 cells. All extrachromosomal and chromosomal recombination events analyzed in mammalian cells resulted from gene conversion. Chromosomal gene conversion events occurred at frequencies of about 10(-6) per cell generation and restored a functional neo gene without overall effects on sequence organization.

scholarly journals Mitotic gene conversion lengths, coconversion patterns, and the incidence of reciprocal recombination in a Saccharomyces cerevisiae plasmid system.

1986 ◽  
Vol 6 (11) ◽  
pp. 3685-3693 ◽  
Author(s):  
B Y Ahn ◽  
D M Livingston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Average Length ◽  
Reciprocal Recombination ◽  
Allelic Sequence ◽  
Flanking Sequences ◽  
Mitotic Gene Conversion ◽  
His3 Gene ◽  
Conversion Tract

Plasmids capable of undergoing genetic exchange in mitotically dividing Saccharomyces cerevisiae cells were used to measure the length of gene conversion events, to determine patterns of coconversion when multiple markers were present, and to correlate the incidence of reciprocal recombination with the length of conversion tracts. To construct such plasmids, restriction site linkers were inserted both within the HIS3 gene and in the flanking sequences, and two different his3- alleles were placed in a vector. Characterization of the genetic exchanges in these plasmids showed that most occur with the conversion of one his3- allele. Many of these events included coconversions in which more than one marker along the allelic sequence was replaced. The frequency of coconversion decreased with the distance between two markers such that markers further than 1 kilobase apart were infrequently coconverted. From these results the average length of conversion was determined to be approximately 0.5 kilobase. Examination of coconversions involving three or more markers revealed an almost obligatory, simultaneous coconversion pattern of all markers. Thus, when two markers which flank an intervening marker are converted, the intervening marker is 20 times more likely to be converted than to remain unchanged. The results of these studies also showed that the incidence of reciprocal recombination, which accompanies more than 20% of the conversion events, is more frequent when the conversion tract is longer than average.

scholarly journals Gene conversion in higher organisms: Non-reciprocal recombination events at the rosy cistron in Drosophila melanogaster

1971 ◽  
Vol 17 (2) ◽  
pp. 139-149 ◽  
Author(s):  
G. H. Ballantyne ◽  
Arthur Chovnick
Keyword(s):  
Drosophila Melanogaster ◽  
Gene Conversion ◽  
Strong Argument ◽  
Reciprocal Recombination ◽  
Regular Event ◽  
Higher Eukaryotes

SUMMARYAnalysis of a series of exceptional ry+ half-tetrads, produced in mass matings involving rosy mutant heterozygous half-tetrads, provides rigorous demonstration of the occurrence of non-reciprocal as well as reciprocal recombination events within the rosy cistron of Drosophila melanogaster. Inferences about allele recombination drawn from this and other studies in Drosophila provide a strong argument that gene conversion occurs as a regular event in higher eukaryotes.

Extrachromosomal and chromosomal gene conversion in mammalian cells

1986 ◽  
Vol 6 (5) ◽  
pp. 1608-1614
Author(s):  
J Rubnitz ◽  
S Subramani
Keyword(s):  
Gene Conversion ◽  
Mammalian Cells ◽  
Insertion Mutation ◽  
Chromosomal Gene ◽  
Sequence Organization ◽  
Reciprocal Recombination ◽  
Homologous Fragment ◽  
Double Recombination ◽  
Neomycin Resistance ◽  
Chromosomal Recombination

We constructed substrates to study gene conversion in mammalian cells specifically without the complication of reciprocal recombination events. These substrates contain both an insertion mutation of the neomycin resistance gene (neoX) and an internal, homologous fragment of the neo gene (neo-526), such that gene conversion from neo-526 to neoX restores a functional neo gene. Although two reciprocal recombination events can also produce an intact neo gene, these double recombination events occur much less frequently that gene conversion in mammalian cells, We used our substrates to characterize extrachromosomal gene conversion in recombination-deficient bacteria and in monkey COS cells. Chromosomal recombination was also studied after stable integration of these substrates into the genome of mouse 3T6 cells. All extrachromosomal and chromosomal recombination events analyzed in mammalian cells resulted from gene conversion. Chromosomal gene conversion events occurred at frequencies of about 10(-6) per cell generation and restored a functional neo gene without overall effects on sequence organization.

A plasmid system to monitor gene conversion and reciprocal recombination in vitro

1993 ◽  
Vol 291 (3) ◽  
pp. 181-192 ◽  
Author(s):  
Tanja Oppliger ◽  
Friedrich E. Würgler ◽  
Christian Sengstag
Keyword(s):  
Gene Conversion ◽  
Reciprocal Recombination
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