Mitotic gene conversion, reciprocal recombination and gene replacement at the benA, beta-tubulin, locus of Aspergillus nidulans

1988 ◽  
Vol 213 (2-3) ◽  
pp. 339-345 ◽  
Author(s):  
Patrick W. Dunne ◽  
Berl R. Oakley
Keyword(s):  
Gene Conversion ◽  
Aspergillus Nidulans ◽  
Gene Replacement ◽  
Beta Tubulin ◽  
Reciprocal Recombination ◽  
Mitotic Gene Conversion

scholarly journals Mitotic gene conversion lengths, coconversion patterns, and the incidence of reciprocal recombination in a Saccharomyces cerevisiae plasmid system.

1986 ◽  
Vol 6 (11) ◽  
pp. 3685-3693 ◽  
Author(s):  
B Y Ahn ◽  
D M Livingston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Average Length ◽  
Reciprocal Recombination ◽  
Allelic Sequence ◽  
Flanking Sequences ◽  
Mitotic Gene Conversion ◽  
His3 Gene ◽  
Conversion Tract

Plasmids capable of undergoing genetic exchange in mitotically dividing Saccharomyces cerevisiae cells were used to measure the length of gene conversion events, to determine patterns of coconversion when multiple markers were present, and to correlate the incidence of reciprocal recombination with the length of conversion tracts. To construct such plasmids, restriction site linkers were inserted both within the HIS3 gene and in the flanking sequences, and two different his3- alleles were placed in a vector. Characterization of the genetic exchanges in these plasmids showed that most occur with the conversion of one his3- allele. Many of these events included coconversions in which more than one marker along the allelic sequence was replaced. The frequency of coconversion decreased with the distance between two markers such that markers further than 1 kilobase apart were infrequently coconverted. From these results the average length of conversion was determined to be approximately 0.5 kilobase. Examination of coconversions involving three or more markers revealed an almost obligatory, simultaneous coconversion pattern of all markers. Thus, when two markers which flank an intervening marker are converted, the intervening marker is 20 times more likely to be converted than to remain unchanged. The results of these studies also showed that the incidence of reciprocal recombination, which accompanies more than 20% of the conversion events, is more frequent when the conversion tract is longer than average.

Mitotic gene conversion lengths, coconversion patterns, and the incidence of reciprocal recombination in a Saccharomyces cerevisiae plasmid system

1986 ◽  
Vol 6 (11) ◽  
pp. 3685-3693
Author(s):  
B Y Ahn ◽  
D M Livingston
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Average Length ◽  
Reciprocal Recombination ◽  
Allelic Sequence ◽  
Flanking Sequences ◽  
Mitotic Gene Conversion ◽  
His3 Gene ◽  
Conversion Tract

Plasmids capable of undergoing genetic exchange in mitotically dividing Saccharomyces cerevisiae cells were used to measure the length of gene conversion events, to determine patterns of coconversion when multiple markers were present, and to correlate the incidence of reciprocal recombination with the length of conversion tracts. To construct such plasmids, restriction site linkers were inserted both within the HIS3 gene and in the flanking sequences, and two different his3- alleles were placed in a vector. Characterization of the genetic exchanges in these plasmids showed that most occur with the conversion of one his3- allele. Many of these events included coconversions in which more than one marker along the allelic sequence was replaced. The frequency of coconversion decreased with the distance between two markers such that markers further than 1 kilobase apart were infrequently coconverted. From these results the average length of conversion was determined to be approximately 0.5 kilobase. Examination of coconversions involving three or more markers revealed an almost obligatory, simultaneous coconversion pattern of all markers. Thus, when two markers which flank an intervening marker are converted, the intervening marker is 20 times more likely to be converted than to remain unchanged. The results of these studies also showed that the incidence of reciprocal recombination, which accompanies more than 20% of the conversion events, is more frequent when the conversion tract is longer than average.

The pso4-1 mutation reduces spontaneous mitotic gene conversion and reciprocal recombination in Saccharomyces cerevisiae

1992 ◽  
Vol 235 (2-3) ◽  
pp. 311-316 ◽  
Author(s):  
L. B. Meira ◽  
M. B. Fonseca ◽  
D. Averbeck ◽  
A. C. G. Schenberg ◽  
J. A. P. Henriques
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Reciprocal Recombination ◽  
Mitotic Gene Conversion

scholarly journals Molecular and cellular evidence for biased mitotic gene conversion in hybrid scallop

2010 ◽  
Vol 10 (1) ◽  
pp. 6 ◽  
Author(s):  
Shi Wang ◽  
Lingling Zhang ◽  
Jingjie Hu ◽  
Zhenmin Bao ◽  
Zhanjiang Liu
Keyword(s):  
Gene Conversion ◽  
Mitotic Gene Conversion

The Escherichia coli recA gene increases UV-induced mitotic gene conversion in Saccharomyces cerevisiae

1994 ◽  
Vol 25 (5) ◽  
pp. 472-474 ◽  
Author(s):  
Viera Vlčková ◽  
Luba Černáková ◽  
Eva Farkašová ◽  
Jela Brozmanová
Keyword(s):  
Escherichia Coli ◽  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Reca Gene ◽  
Mitotic Gene Conversion

High-frequency homologous recombination between duplicate chromosomal immunoglobulin mu heavy-chain constant regions

1989 ◽  
Vol 9 (12) ◽  
pp. 5500-5507
Author(s):  
M D Baker
Keyword(s):  
Homologous Recombination ◽  
Heavy Chain ◽  
High Frequency ◽  
Gene Replacement ◽  
Immunoglobulin M ◽  
Hybridoma Cells ◽  
Immunoglobulin Gene ◽  
Reciprocal Recombination ◽  
Base Pair Deletion ◽  
Frequent Event

Homologous recombination was used in a previous study to correct a 2-base-pair deletion in the third constant domain (Cmu3) of the haploid chromosomal mu gene in a mutant hybridoma cell line by transfer of a pSV2neo vector bearing a subfragment of the normal Cmu region (M.D. Baker, N. Pennell, L. Bosnoyan, and M.J. Shulman, Proc. Natl. Acad. Sci. USA 85:6432-6436, 1988). In these experiments, both gene replacement and single reciprocal crossover events were found to restore normal, cytolytic 2,4,6-trinitrophenyl-specific immunoglobulin M production to the mutant cells. In the cases of single reciprocal recombination, the structure of the recombinant mu gene is such that the normal Cmu region, in its correct position 3' of the expressed 2,4,6-trinitrophenyl-specific heavy-chain variable region, is separated from the mutant Cmu region by the integrated vector sequences. I report here that homologous recombination occurs with high frequency between the duplicate Cmu regions in mitotically growing hybridoma cells. The homologous recombination events were easily detected since they generated hybridomas that were phenotypically different from the parental cells. Analysis of the recombinant cells suggests that gene conversion is the most frequent event, occurring between 60 and 73% of the time. The remaining events consisted of single reciprocal crossovers. Intrachromatid double reciprocal recombination was not detected. The high frequency of recombination, the ability to isolate and analyze the participants in the recombination reactions, and the capacity to generate specific modifications in the immunoglobulin Cmu regions by gene targeting suggest that this system will be useful for studying mammalian chromosomal homologous recombination. Moreover, the ability to specifically modify the chromosomal immunoglobulin genes by homologous recombination should facilitate studies of immunoglobulin gene regulation and expression and provide a more convenient of engineering specifically modified antibody.

Evidence for Independent Mismatch Repair Processing on Opposite Sides of a Double-Strand Break in Saccharomyces cerevisiae

Genetics ◽  
1998 ◽  
Vol 148 (1) ◽  
pp. 59-70
Author(s):  
Yi-shin Weng ◽  
Jac A Nickoloff
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Gene Conversion ◽  
Mismatch Repair ◽  
Strand Break ◽  
Double Strand Break ◽  
Stem Loop ◽  
Mitotic Gene Conversion ◽  
Low Levels ◽  
Mat Locus ◽  
Insertion Mutations

Abstract Double-strand break (DSB) induced gene conversion in Saccharomyces cerevisiae during meiosis and MAT switching is mediated primarily by mismatch repair of heteroduplex DNA (hDNA). We used nontandem ura3 duplications containing palindromic frameshift insertion mutations near an HO nuclease recognition site to test whether mismatch repair also mediates DSB-induced mitotic gene conversion at a non-MAT locus. Palindromic insertions included in hDNA are expected to produce a stem-loop mismatch, escape repair, and segregate to produce a sectored (Ura+/−) colony. If conversion occurs by gap repair, the insertion should be removed on both strands, and converted colonies will not be sectored. For both a 14-bp palindrome, and a 37-bp near-palindrome, ~75% of recombinant colonies were sectored, indicating that most DSB-induced mitotic gene conversion involves mismatch repair of hDNA. We also investigated mismatch repair of well-repaired markers flanking an unrepaired palindrome. As seen in previous studies, these additional markers increased loop repair (likely reflecting corepair). Among sectored products, few had additional segregating markers, indicating that the lack of repair at one marker is not associated with inefficient repair at nearby markers. Clear evidence was obtained for low levels of short tract mismatch repair. As seen with full gene conversions, donor alleles in sectored products were not altered. Markers on the same side of the DSB as the palindrome were involved in hDNA less often among sectored products than nonsectored products, but markers on the opposite side of the DSB showed similar hDNA involvement among both product classes. These results can be explained in terms of corepair, and they suggest that mismatch repair on opposite sides of a DSB involves distinct repair tracts.

Sign in / Sign up

Export Citation Format

Share Document