scholarly journals Comparative Field Studies of Various Traps and Attractants for the Olive Fruit fly, Bαctrocerα oleαe

2017 ◽  
Vol 12 ◽  
pp. 71 ◽  
Author(s):  
G.E. Haniotakis ◽  
ΤΗ. Broumas ◽  
C. Liaropoulos
Keyword(s):  
Uv Light ◽  
Fruit Fly ◽  
Field Studies ◽  
Ammonium Bicarbonate ◽  
Protein Hydrolysates ◽  
Olive Fruit Fly ◽  
Mass Trapping ◽  
Olive Fruit ◽  
Olive Fly ◽  
High Concentrations

In the framework of an ongoing effort for development of an effective trap for the control of the olive fruit fly, Bactrocera oleae (Gmelin) (Diptera, Tephrifidae), by mass trapping, four trap types (wood, cloth, laminate, McPhail), seven different colors of laminate traps, different food attractants, attractant combinations or formulations (total of ten cases), and two insecticides (deltamethrin, b-cyfluthrin) used as killing agents on the traps, were compared under field conditions. The following conclusions may be drawn: Laminate traps (paper envelopes with a polyethylene lining inside, 15×20 cm in size, containing 70 g of ammonium bicarbonate salt and impregnated with 15 mg (a.i.) of an insecticide), were more effective than wood traps. Effectiveness of laminate traps can be enhanced by using an attracting color or in the case of the food attractant ammonium bicarbonate, by incorporating copper oxychloride. Combinations of two protein hydrolysates were more effective than one hydrolysate alone. Effectiveness of protein hydrolysates is not constantly higher than that of ammonium bicarbonate, the use of which is more economic and convenient. The insecticide b-cyfluthrin may be used on traps instead of deltamethrin, which is known to have a repellent effect on the olive fly, especially Linder high concentrations, and is unstable under natural UV light.

scholarly journals Yeasts Associated with the Olive Fruit Fly Bactrocera oleae (Rossi) (Diptera: Tephritidae) Lead to New Attractants

Agronomy ◽  
2020 ◽  
Vol 10 (10) ◽  
pp. 1501
Author(s):  
Elda Vitanović ◽  
Julian M. Lopez ◽  
Jeffrey R. Aldrich ◽  
Maja Jukić Špika ◽  
Kyria Boundy-Mills ◽  
...  
Keyword(s):  
Candida Utilis ◽  
Insect Pest ◽  
Fruit Fly ◽  
Bactrocera Oleae ◽  
Olive Fruit Fly ◽  
Olive Fruit ◽  
Torula Yeast ◽  
Yeast Cultures ◽  
Industry Standard ◽  
Olive Fly

The olive fruit fly (Bactrocera oleae Rossi) is the primary insect pest in all olive-growing regions worldwide. New integrated pest management (IPM) techniques are needed for B. oleae to mitigate reliance on pesticides used for its control which can result in negative environmental impacts. More effective lures for monitoring olive flies would help to know when and where direct chemical applications are required. The aim of this research was to find new, more effective methods for B. oleae detection and monitoring. Twelve insect-associated yeasts were selected and tested as living cultures in McPhail traps for the attraction of olive flies. Certain yeasts were more attractive than others to B. oleae; specifically, Kuraishia capsulata, Lachancea thermotolerans, Peterozyma xylosa, Scheffersomyces ergatensis, and Nakazawae ernobii, than the industry-standard dried torula yeast (Cyberlindnera jadinii; syn. Candida utilis). The attractiveness of dry, inactive (i.e., non-living) formulations of these five yeasts was also tested in the field. Inactive formulations of K. capsulata, P. xylosa, N. ernobii, and L. thermotolerans were significantly more attractive to B. oleae than commercially available torula yeast. Green lacewing, Chrysoperla comanche (Stephens) (Neuroptera: Chrysopidae), adults were incidentally caught in traps baited with the live yeast cultures. This is the first field study that compares olive fly attraction to yeast species other than torula yeast. Commercialization of yeasts that are more attractive than the torula standard would improve monitoring and associated control of the olive fruit fly.

scholarly journals Transcriptome landscape of the developing olive fruit fly embryo delineated by Oxford Nanopore long-read RNA-Seq

2018 ◽  
Author(s):  
Anthony Bayega ◽  
Spyros Oikonomopoulos ◽  
Eleftherios Zorbas ◽  
Yu Chang Wang ◽  
Maria-Eleni Gregoriou ◽  
...  
Keyword(s):  
Embryo Development ◽  
De Novo ◽  
Transcriptome Assembly ◽  
Fruit Fly ◽  
Full Length ◽  
Olive Fruit Fly ◽  
Rna Seq ◽  
Olive Fruit ◽  
Olive Fly ◽  
Oxford Nanopore

AbstractThe olive fruit fly or olive fly (Bactrocera oleae) is the most important pest of cultivated olive trees. Like all insects the olive fly undergoes complete metamorphosis. However, the transcription dynamics that occur during early embryonic development have not been explored, while detailed transcriptomic analysis in the absence of a fully annotated genome is challenging. We collected olive fly embryos at hourly intervals for the first 6 hours of development and performed full-length cDNA-Seq using a purpose designed SMARTer cDNA synthesis protocol followed by sequencing on the MinION (Oxford Nanopore Technologies). We generated 31 million total reads across the timepoints (median yield 4.2 million per timepoint). The reads showed 98 % alignment rate to the olive fly genome and 91 % alignment rate to the NBCI predicted B. oleae gene models. Over 50 % of the expressed genes had at least one read covering its entire length validating our full-length RNA-Seq procedure. Expression of 68 % of the predicted B. oleae genes was detected in the first six hours of development. We generated a de novo transcriptome assembly of the olive fly and identified 3553 novel genes and a total of 79,810 transcripts; a fourfold increase in transcriptome diversity compared to the NCBI predicted transcriptome. On a global scale, the first six hours of embryo development were characterized by dramatic transcriptome changes with the total number of transcripts per embryo dropping to half from the first hour to the second hour of embryo development. Clustering of genes based on temporal co-expression followed by gene-set enrichment analysiss of genes expressed in the first six hours of embryo development showed that genes involved in transcription and translation, macro-molecule biosynthesis, and neurodevelopment were highly enriched. These data provide the first insight into the transcriptome landscape of the developing olive fly embryo. The data also reveal transcript signatures of sex development. Overall, full-length sequencing of the cDNA molecules permitted a detailed characterization of the isoform complexity and the transcriptional dynamics of the first embryonic stages of the B. oleae.

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