scholarly journals Genome Size of Twenty Wild Species of Oryza Determined by Flow Cytometric and Chromosome Analyses

2007 ◽  
Vol 57 (1) ◽  
pp. 73-78 ◽  
Author(s):  
Toshie Miyabayashi ◽  
Ken-Ichi Nonomura ◽  
Hiroko Morishima ◽  
Nori Kurata
Keyword(s):  
Genome Size ◽  
Wild Species ◽  
Flow Cytometric

scholarly journals Flow Cytometric Determination of Genome Size in the Taxodiaceae, Cupressaceae sensu stricto and Sciadopityaceae.

CYTOLOGIA ◽  
2001 ◽  
Vol 66 (3) ◽  
pp. 307-311 ◽  
Author(s):  
Masahiro Hizume ◽  
Teiji Kondo ◽  
Fukashi Shibata ◽  
Ryoko Ishizuka
Keyword(s):  
Genome Size ◽  
Sensu Stricto ◽  
Flow Cytometric

The Genome Size of Some Species of Hyalomma Ticks from Turkish Thrace 

2018 ◽  
Vol 23 (11) ◽  
pp. 2122 ◽  
Author(s):  
Nadim Yılmazer
Keyword(s):  
Genome Size ◽  
X Chromosome ◽  
Tick Species ◽  
Animal Health ◽  
Chromosome Size ◽  
Diploid Genome ◽  
Flow Cytometric ◽  
Determination System ◽  
Sex Determination System ◽  
First Time

Hyalomma scupense, H. excavatum and H. marginatum are globally important tick species, as well as in Turkey, both in terms of human and animal health. The genome sizes of these tick species were determined in this study for the first time. From flow cytometric measurements, diploid genome sizes of female and male H. scupense were found to be 2.13 pg and 1.75 pg, respectively, while H. excavatum were 2.21 pg and 1.94 pg, and H. marginatum were 2.48 pg and 1.98 pg, respectively. Differences in diploid genome size indicate X chromosome size of females and males in these ticks because they have an XX:XO sex determination system. Thus, it was estimated that the X chromosome of H. scupense, H. excavatum, and H. marginatum may be composed of as much as 0.38 pg, 0.27 pg, and 0.50 pg of DNA, respectively. These findings indicate suitability of these three species for genome sequencing due to the relatively small size of their genomes compared with other tick species.

Genome size and nucleotypic variation in Malus germplasm

Genome ◽  
2009 ◽  
Vol 52 (2) ◽  
pp. 148-155 ◽  
Author(s):  
Schuyler S. Korban ◽  
Wannasiri Wannarat ◽  
Charlotte M. Rayburn ◽  
Tatiana C. Tatum ◽  
A. Lane Rayburn
Keyword(s):  
Genome Size ◽  
Flow Cytometric Analysis ◽  
Internal Standard ◽  
The United States ◽  
Mean Value ◽  
Ploidy Levels ◽  
Flow Cytometric ◽  
Malus Species ◽  
Leaf Tissues ◽  
Stomatal Length

The genus Malus has anywhere between 25 and 33 species along with several subspecies. Malus species as well as clones within the same species have varying ploidy levels, as these are more than likely collected from different trees and (or) from different locations. In recent years, large numbers of Malus germplasm accessions have been collected and maintained at the United States National Germplasm Clonal Repository; however, genome sizes of this material have not yet been determined. In this study, leaf tissues from young grafted trees of 100 Malus species and hybrids growing in a nursery at the University of Illinois were collected and immediately used for extracting nuclei. Leaf tissues from apple and maize line W-22, used as an internal standard, were co-chopped and prepared for flow cytometric analysis. Apple nuclei were stained with propidium iodide, an intercalating dye, and a minimum of 8000 nuclei per sample were analyzed. Mean fluorescence of apple nuclei was then determined. A total of four replications per sample was used. Among 100 Malus accessions analyzed, one tetraploid, three triploid, and 96 diploid genotypes were identified. Significant differences in genome size were identified among the three ploidy types observed and also within diploid genotypes. The 2C mean value for tetraploids was 3.13 pg and ranged from 2.27 to 2.41 pg for triploids, whereas 2C values for diploids ranged between 1.44 and 1.72 pg. In addition, leaf impressions of young, fully expanded leaves were collected from young trees of 10 selected genotypes based on their ploidy and flow cytometric analysis and used to measure the nucleotypic parameter stomatal length. Ten stomata were measured per slide, three slides were analyzed per leaf, and three leaves were analyzed per accession. Overall, mean length of stomata ranged between 19.47 μm (diploid) and 27.6 μm (tetraploid), indicating that stomatal length in a tetraploid Malus genotype was 1.4-fold higher than that of a diploid genotype. A positive correlation between genome size and the nucleotypic parameter stomatal length was observed.

Genome size variation among and within Ophiopogoneae species by flow cytometric analysis

2017 ◽  
Vol 40 (2) ◽  
pp. 529-537
Author(s):  
Guangyan Wang ◽  
Ying Meng ◽  
Yongping Yang
Keyword(s):  
Genome Size ◽  
Flow Cytometric Analysis ◽  
Size Variation ◽  
Genome Size Variation ◽  
Flow Cytometric

Flow cytometric and Feulgen densitometric analysis of genome size variation in Pisum

1996 ◽  
Vol 92 (3-4) ◽  
pp. 297-307 ◽  
Author(s):  
M. Baranyi ◽  
J. Greilhuber
Keyword(s):  
Genome Size ◽  
Size Variation ◽  
Genome Size Variation ◽  
Densitometric Analysis ◽  
Flow Cytometric

Diploid and tetraploid cytotypes and subspecies of Odontarrhena tortuosa (Brassicaceae) in Pannonia: differences in morphology, ecology and genome size

Phytotaxa ◽  
2018 ◽  
Vol 358 (2) ◽  
pp. 162
Author(s):  
STANISLAV ŠPANIEL ◽  
LÝDIA HABURAJOVÁ ◽  
VERONIKA CETLOVÁ
Keyword(s):  
Genome Size ◽  
Central Europe ◽  
Morphological Variation ◽  
Pannonian Basin ◽  
Sand Dunes ◽  
Distribution Area ◽  
Flow Cytometric Data ◽  
Flow Cytometric ◽  
Morphological Differences ◽  
Rocky Habitats

Odontarrhena tortuosa is a herbaceous species occurring in steppe and rocky habitats of Eurasia. The present study is focused on the cytogeography and morphological variation of the species’ populations growing in the Pannonian basin (Central Europe), which represents the westernmost part of its distribution area. Flow cytometric analyses have revealed the existence of tetraploid populations, which are reported here for the first time for Central Europe. We analysed the morphological variation of 329 individuals from Slovakia, Hungary and Serbia by multivariate morphometric methods to evaluate morphological differences between two cytotypes (diploids and tetraploids), between the subspecies (O. tortuosa subsp. tortuosa and O. tortuosa subsp. heterophylla) and between populations growing in three regions differing in the type of substrate (rocks, sand dunes and sandy and rocky screes). Although we have not found any clear morphological differences between the cytotypes, we have confirmed several previously reported morphological differences between the two subspecies, which occur in regions differing in the type of substrate. Flow cytometric data also indicate certain differences in monoploid genome size between all groups of populations under comparison. Populations from Serbia differ from other populations of O. tortuosa subsp. tortuosa in morphology and genome size, but due to a lack of data on the genetic variation of the species we suggest no change in taxonomy. For the identification of the two subspecies, we present an updated determination key.

scholarly journals Flow cytometric determination of genome size in European sunbleak Leucaspius delineatus (Heckel, 1843)

2011 ◽  
Vol 38 (2) ◽  
pp. 355-362 ◽  
Author(s):  
Marta Filipiak ◽  
Grzegorz Tylko ◽  
Wincenty Kilarski
Keyword(s):  
Genome Size ◽  
Flow Cytometric

Flow cytometric determination of genome size in Pinus

Plant Science ◽  
1996 ◽  
Vol 115 (1) ◽  
pp. 91-99 ◽  
Author(s):  
Iona E.W. O'Brien ◽  
Dale R. Smith ◽  
Richard C. Gardner ◽  
Brian G. Murray
Keyword(s):  
Genome Size ◽  
Flow Cytometric

Flow cytometric determination of genome size for eight commercially important fish species in China

2012 ◽  
Vol 48 (8) ◽  
pp. 507-517 ◽  
Author(s):  
Dongmei Zhu ◽  
Wen Song ◽  
Kun Yang ◽  
Xiaojuan Cao ◽  
Yasmeen Gul ◽  
...  
Keyword(s):  
Genome Size ◽  
Fish Species ◽  
Flow Cytometric ◽  
Commercially Important ◽  
Important Fish
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