scholarly journals Cytoplasmic male sterile lines of a tobacco variety, Tsukuba 1, developed by asymmetric protoplast fusion.

1988 ◽  
Vol 38 (2) ◽  
pp. 158-164 ◽  
Author(s):  
Tomoaki Kubo ◽  
Takashi Kumashiro ◽  
Yasuhito Saito
Keyword(s):  
Protoplast Fusion ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Asymmetric Protoplast Fusion

scholarly journals An asymmetric protoplast fusion and screening method for generating celeriac cybrids

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Silvia Bruznican ◽  
Tom Eeckhaut ◽  
Johan Van Huylenbroeck ◽  
Ellen De Keyser ◽  
Hervé De Clercq ◽  
...  
Keyword(s):  
Protoplast Fusion ◽  
Screening Method ◽  
Low Frequency ◽  
Nuclear Genome ◽  
Hybrid Seed Production ◽  
Male Sterile ◽  
Suspension Cells ◽  
Asymmetric Protoplast Fusion ◽  
Pooled Samples ◽  
Hybrid Screening

AbstractCeleriac F1 hybrid seed production is currently complicated due to the instability of cytoplasmic male sterile lines. To develop alternative alloplasmic CMS lines, an asymmetric protoplast fusion and hybrid screening methodology was established. Celeriac suspension cells protoplasts were used as the acceptor and carrot, coriander and white celery mesophyll protoplasts as the donor for protoplast fusion experiments. Acceptor cytoplasmic inheritance was inhibited by iodoacetamide treatment and donor nuclear genome inheritance was prevented by UV exposure. Protoplasts were selectively stained and fused using electroporation and polyethylene glycol, and candidate hybrid shoots were obtained. One chloroplast and three mitochondrial markers that could distinguish acceptor and donors organelles were used to characterize over 600 plants obtained after fusion events, without identifying any cybrid. In order to increase the testing efficiency a high number of micro plantlets were pooled and hence the presence of the carrot specific Atp1 marker in one of the pooled samples was detected. We demonstrated that fusion took place between celeriac and a carrot indicating that the creation of viable hybrids is possible although at a very low frequency. These findings open the path for new cytoplasmic hybridization and the isolation of novel CMS lines of celeriac.

Cloning and Expression Analysis of cMDH Gene Related to Cytoplasmic Male Sterile Wheat with Aegilops kotschyi Cytoplasm

2009 ◽  
Vol 35 (9) ◽  
pp. 1620-1627 ◽  
Author(s):  
Long-Yu ZHANG ◽  
Hong-Xia LI ◽  
Gai-Sheng ZHANG ◽  
Jun-Sheng WANG ◽  
Yan-Fen HAN ◽  
...  
Keyword(s):  
Expression Analysis ◽  
Cloning And Expression ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

Fertility and Cytomorphology of Anthers in New Cytoplasmic Male-Sterile Lines of Chinese Cabbage(Brassica campestris L. ssp. pekinensis)

2012 ◽  
Vol 30 (1) ◽  
pp. 49
Author(s):  
Zhan SHI ◽  
Zheng-Jie WAN ◽  
Yue-Jin XU ◽  
Rui-Chang ZOU ◽  
Tao HUANG ◽  
...  
Keyword(s):  
Chinese Cabbage ◽  
Brassica Campestris ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile

scholarly journals Development of cytoplasmic male sterile lines and restorer lines of various elite Indica Group rice cultivars using CW-CMS/Rf17 system

Rice ◽  
2019 ◽  
Vol 12 (1) ◽  
Author(s):  
Kinya Toriyama ◽  
Tomohiko Kazama ◽  
Tadashi Sato ◽  
Yoshimichi Fukuta ◽  
Masaaki Oka
Keyword(s):  
Nuclear Gene ◽  
Oryza Rufipogon ◽  
Rice Cultivars ◽  
Male Sterile ◽  
Cytoplasmic Male Sterile ◽  
Cms Line

Abstract Background A cytoplasm of CW-type cytoplasmic male sterile (CMS) line is derived from Oryza rufipogon strain W1 and fertility is restored by a single nuclear gene, Rf17. We have previously reported that CW-CMS were effective for breeding CMS lines of Indica Group rice cultivars, IR 24 and IR 64. The applicability of this CW-CMS/Rf17 system to produce other elite Indica Group rice cultivars with CMS was explored. Findings Out of seven elite Indica Group rice cultivars, complete CMS lines were obtained for six cultivars: NSIC Rc 160, NSIC Rc 240, Ciherang, BRRI dhan 29, NERICA-L-19, and Pusa Basmati. The fertility of these six lines was restored when Rf17 was present. A CMS line was not obtained for the cultivar Samba Mahsuri. Conclusions The CW-CMS/Rf17 system will be useful to produce CMS lines and restorer lines of various elite Indica Group rice cultivars.

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