scholarly journals Cyclic Tetrasaccharide-Synthesizing Enzymes fromArthrobacter globiformisA19

2004 ◽  
Vol 68 (12) ◽  
pp. 2529-2540 ◽  
Author(s):  
Kazuhisa MUKAI ◽  
Kazuhiko MARUTA ◽  
Kazuhiro SATOUCHI ◽  
Michio KUBOTA ◽  
Shigeharu FUKUDA ◽  
...  
Keyword(s):  
Cyclic Tetrasaccharide

Evaluation of the Relative Available Energy of Cyclic Nigerosylnigerose (CNN) using breath hydrogen excretion in healthy humans

2021 ◽  
Author(s):  
Akiko Mizote ◽  
Akiko Yasuda ◽  
Chiyo Yoshizane ◽  
Yuki Ishida ◽  
Shoji Kakuta ◽  
...  
Keyword(s):  
Brush Border Membrane ◽  
Human Subjects ◽  
Membrane Vesicles ◽  
Controlled Study ◽  
Breath Hydrogen ◽  
Double Blind ◽  
Available Energy ◽  
Healthy Humans ◽  
Cyclic Tetrasaccharide ◽  
Study Participants

Abstract Cyclic nigerosylnigerose (CNN) is a cyclic tetrasaccharide with properties distinct from those of other conventional cyclodextrins. We investigated the relative available energy of CNN in healthy humans. CNN digestibility was determined using brush border membrane vesicles from the small intestines of rats. CNN was not hydrolyzed by rat intestinal enzymes. To investigate breath hydrogen excretion, thirteen human subjects were included in a double-blind cross-over, randomized, placebo-controlled study. The effects of CNN on hydrogen excretion were compared with those of a typical nondigestible, fermentable fructooligosaccharide (FOS). In the study participants, hydrogen excretion hardly increased upon CNN and was remarkably lower than for FOS. The available energy value was determined using the fermentability based on breath hydrogen excretion and was evaluated as 0 kcal/g for CNN. CNN was hardly metabolized and hence may be used as a low-energy dietary fiber.

Production of Cyclic Tetrasaccharide with 6-?-Glucosyltransferase and ?-Isomaltosyltransferase

ChemInform ◽  
2005 ◽  
Vol 36 (21) ◽  
Author(s):  
Tomoyuki Nishimoto ◽  
Hajime Aga ◽  
Michio Kubota ◽  
Shigeharu Fukuda ◽  
Masashi Kurimoto ◽  
...  
Keyword(s):  
Cyclic Tetrasaccharide

Enzymatic synthesis of a β-d-galactopyranosyl cyclic tetrasaccharide by β-galactosidases

2004 ◽  
Vol 339 (9) ◽  
pp. 1603-1608 ◽  
Author(s):  
Takanobu Higashiyama ◽  
Hikaru Watanabe ◽  
Hajime Aga ◽  
Tomoyuki Nishimoto ◽  
Michio Kubota ◽  
...  
Keyword(s):  
Enzymatic Synthesis ◽  
Cyclic Tetrasaccharide

An enzymatically produced novel cyclic tetrasaccharide, cyclo-{→6)-α-d-Glcp-(1→4)-α-d-Glcp-(1→6)-α-d-Glcp-(1→4)-α-d-Glcp-(1→} (cyclic maltosyl-(1→6)-maltose), from starch

2005 ◽  
Vol 340 (8) ◽  
pp. 1469-1474 ◽  
Author(s):  
Kazuhisa Mukai ◽  
Hikaru Watanabe ◽  
Kazuyuki Oku ◽  
Tomoyuki Nishimoto ◽  
Michio Kubota ◽  
...  
Keyword(s):  
Cyclic Tetrasaccharide

Supramolecular Photochirogenesis with Novel Cyclic Tetrasaccharide: Enantiodifferentiating Photoisomerization of (Z)-Cyclooctene with Cyclic Nigerosylnigerose-Based Sensitizers

Chirality ◽  
2012 ◽  
Vol 24 (11) ◽  
pp. 921-927 ◽  
Author(s):  
Cheng Yang ◽  
Wenting Liang ◽  
Masaki Nishijima ◽  
Gaku Fukuhara ◽  
Tadashi Mori ◽  
...  
Keyword(s):  
Cyclic Tetrasaccharide

scholarly journals Purification, Characterization, and Gene Cloning of a Novel Maltosyltransferase from an Arthrobacter globiformis Strain That Produces an Alternating α-1,4- and α-1,6-Cyclic Tetrasaccharide from Starch

2006 ◽  
Vol 72 (2) ◽  
pp. 1065-1071 ◽  
Author(s):  
Kazuhisa Mukai ◽  
Hikaru Watanabe ◽  
Michio Kubota ◽  
Hiroto Chaen ◽  
Shigeharu Fukuda ◽  
...  
Keyword(s):  
Amino Acids ◽  
Molecular Mass ◽  
Genomic Library ◽  
Soluble Starch ◽  
Transfer Reactions ◽  
Arthrobacter Globiformis ◽  
Calculated Molecular Mass ◽  
Cyclic Tetrasaccharide ◽  
Cyclomaltodextrin Glucanotransferase ◽  
Thermal Stability Of

ABSTRACT A glycosyltransferase, involved in the synthesis of cyclic maltosylmaltose [CMM; cyclo-{→6)-α-d-Glcp(1→4)-α-d-Glcp(1→6)-α-d-Glcp(1→4)-α-d-Glcp(1→}] from starch, was purified to homogeneity from the culture supernatant of Arthrobacter globiformis M6. The CMM-forming enzyme had a molecular mass of 71.7 kDa and a pI of 3.6. The enzyme was most active at pH 6.0 and 50°C and was stable from pH 5.0 to 9.0 and up to 30°C. The addition of 1 mM Ca2+ enhanced the thermal stability of the enzyme up to 45°C. The enzyme acted on maltooligosaccharides that have degrees of polymerization of ≥3, amylose, and soluble starch to produce CMM but failed to act on cyclomaltodextrins, pullulan, and dextran. The mechanism for the synthesis of CMM from maltotetraose was determined as follows: (i) maltotetraose + maltotetraose → 64-O-α-maltosyl-maltotetraose + maltose and (ii) 64-O-α-maltosyl-maltotetraose → CMM + maltose. Thus, the CMM-forming enzyme was found to be a novel maltosyltransferase (6MT) catalyzing both intermolecular and intramolecular α-1,6-maltosyl transfer reactions. The gene for 6MT, designated cmmA, was isolated from a genomic library of A. globiformis M6. The cmmA gene consisted of 1,872 bp encoding a signal peptide of 40 amino acids and a mature protein of 583 amino acids with a calculated molecular mass of 64,637. The deduced amino acid sequence showed similarities to α-amylase and cyclomaltodextrin glucanotransferase. The four conserved regions common in the α-amylase family enzymes were also found in 6MT, indicating that 6MT should be assigned to this family.

scholarly journals Transglycosylation of Glycosyl Residues to Cyclic Tetrasaccharide byBacillus stearothermophilusCyclomaltodextrin Glucanotransferase Using Cyclomaltodextrin as the Glycosyl Donor

2003 ◽  
Vol 67 (5) ◽  
pp. 1094-1100 ◽  
Author(s):  
Takashi SHIBUYA ◽  
Hajime AGA ◽  
Hikaru WATANABE ◽  
Tomohiko SONODA ◽  
Michio KUBOTA ◽  
...  
Keyword(s):  
Glycosyl Donor ◽  
Cyclic Tetrasaccharide

X-ray structure determination and modeling of the cyclic tetrasaccharide cyclo-{→6)-α-d-Glcp-(1→3)-α-d-Glcp-(1→6)-α-d-Glcp-(1→3)-α-d-Glcp-(1→}

2000 ◽  
Vol 329 (3) ◽  
pp. 655-665 ◽  
Author(s):  
Gail M. Bradbrook ◽  
Katrin Gessler ◽  
Gregory L. Côté ◽  
Frank Momany ◽  
Peter Biely ◽  
...  
Keyword(s):  
Structure Determination ◽  
X Ray ◽  
Cyclic Tetrasaccharide

Diastereodifferentiating Photocyclodimerization of 2-Anthracenecarboxylates Tethered to a Cyclic Tetrasaccharide Scaffold: Critical Control of Photoreactivity and Stereoselectivity

2013 ◽  
Vol 78 (21) ◽  
pp. 10996-11006 ◽  
Author(s):  
Gaku Fukuhara ◽  
Tomohiro Nakamura ◽  
Yuko Kawanami ◽  
Cheng Yang ◽  
Tadashi Mori ◽  
...  
Keyword(s):  
Cyclic Tetrasaccharide
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