Production of Recombinant Protein Using the HeLa S3-Vaccinia Virus Expression System: Bioreactor Perfusion and Effects of Post-Infection Temperature

2005 ◽  
Vol 69 (6) ◽  
pp. 1065-1072 ◽  
Author(s):  
Nicole A. BLECKWENN ◽  
William E. BENTLEY ◽  
Joseph SHILOACH
Keyword(s):  
Recombinant Protein ◽  
Vaccinia Virus ◽  
Post Infection ◽  
Expression System ◽  
Hela S3 ◽  
Virus Expression

High Level Expression of Active Human Prothrombin in a Vaccina Virus Expression System

1992 ◽  
Vol 68 (02) ◽  
pp. 119-124 ◽  
Author(s):  
F G Falkner ◽  
P L Turecek ◽  
R T A MacGillivray ◽  
W Bodemer ◽  
F Scheiflinger ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Cell Lines ◽  
Expression System ◽  
Human Cell Line ◽  
Cell System ◽  
Time Saving ◽  
Expression Levels ◽  
Mammalian Cell Lines ◽  
Cell Line Hela ◽  
Virus Expression

SummaryWe have worked out an efficient and time saving procedure for the expression of recombinant human prothrombin. The glycoprotein was expressed in the vaccinia virus expression system in several mammalian cell lines. The kidney cell lines Vero and BHK and the human cell line Hela were found to efficiently secrete prothrombin. Expression levels of 3–4 µg of factor II per 106 cells per day corresponding to 18–23 mU per 106 cells per day were achieved. Since the expression levels obtained with the vaccinia virus/Vero cell system were comparable to those obtained in amplified transformed CHO cells it provides an alternative system for the efficient expression of human prothrombin and may allow to further elucidate structure-function relationships of (pro)thrombin and its various effectors.

Study of the murine Hox-2.3 protein using a vaccinia virus expression system

1989 ◽  
Vol 27 ◽  
pp. 186
Author(s):  
Ron de Jong ◽  
Lia de Laaf ◽  
Harry Vennema ◽  
Willy Spaan ◽  
Jacqueline Deschamps ◽  
...  
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Virus Expression

Functional Characterization of Glycosylation-Deficient Human P-Glycoprotein Using A Vaccinia Virus Expression System

2000 ◽  
Vol 173 (3) ◽  
pp. 203-214 ◽  
Author(s):  
J.J. Gribar ◽  
M. Ramachandra ◽  
C.A. Hrycyna ◽  
S. Dey ◽  
S.V. Ambudkar
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Functional Characterization ◽  
P Glycoprotein ◽  
Virus Expression

Synthesis and secretion of a functional antibody in a vaccinia virus expression system

1992 ◽  
Vol 29 (7-8) ◽  
pp. 821-827 ◽  
Author(s):  
Anthony R. Carroll ◽  
David J. Rowlands ◽  
Berwyn E. Clarke
Keyword(s):  
Vaccinia Virus ◽  
Expression System ◽  
Virus Expression

ATM protein purified from vaccinia virus expression system: DNA binding requirements for kinase activation

2004 ◽  
Vol 322 (1) ◽  
pp. 74-81 ◽  
Author(s):  
Helen H. Chun ◽  
Robert B. Cary ◽  
Fredrick Lansigan ◽  
Julian Whitelegge ◽  
David J. Rawlings ◽  
...  
Keyword(s):  
Dna Binding ◽  
Vaccinia Virus ◽  
Expression System ◽  
Kinase Activation ◽  
Atm Protein ◽  
Virus Expression
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