scholarly journals S-sulfocysteine as a useful sulfur source for cephalosporin C biosynthesis by Cephalosporium acremonium.

1982 ◽  
Vol 46 (6) ◽  
pp. 1519-1523 ◽  
Author(s):  
Yukio FUJISAWA ◽  
Minoru UCHIDA ◽  
Masaru SUZUKI
Keyword(s):  
Sulfur Source ◽  
Cephalosporin C ◽  
Cephalosporium Acremonium

scholarly journals The stereochemistry of β-lactam formation in cephalosporin biosynthesis

1978 ◽  
Vol 169 (3) ◽  
pp. 705-707 ◽  
Author(s):  
J A Huddleston ◽  
E P Abraham
Keyword(s):  
Cephalosporin C ◽  
Cephalosporium Acremonium ◽  
Beta Lactam ◽  
Lactam Ring

3H and 14C from (2R,3S)[U-14C,3-3H1]cysteine and (2R,3R)-[U-14C,2,3-3H2]cysteine were incorporated into cephalosporin C by Cephalosporium acremonium. Analysis of the radioactive cephalosporin C indicated that the formation of its beta-lactam ring occurs stereospecifically and with retention of configuration at C-3 of cysteine.

Sulfatase regulation and antibiotic synthesis in Cephalosporium acremonium

1969 ◽  
Vol 15 (2) ◽  
pp. 175-181 ◽  
Author(s):  
David W. Dennen ◽  
Diane D. Carver
Keyword(s):  
Amino Acid ◽  
Culture Medium ◽  
Sulfur Compounds ◽  
Cephalosporin C ◽  
Cephalosporium Acremonium ◽  
Antibiotic Synthesis ◽  
Cellular Processes ◽  
High Level ◽  
Sulfur Containing ◽  
Sulfur Containing Compounds

The sulfatase of Cephalosporium acremonium is regulated by exogenous sulfur compounds, repressed in cells in 0.02 M sulfate, and derepressed in 5 × 10−4 M sulfate. Organic sulfur sources, such as cysteine, homocysteine, and methionine, derepress the enzyme in varying degrees while the latter amino acid is also required for maximum synthesis of the antibiotics cephalosporin C and penicillin N. Sulfatase-repressed cells transferred from sulfate to methionine-containing medium produce a high level of these antibiotics in the culture medium and a proportionate derepression of the sulfatase. Cycloheximide inhibits sulfatase derepression in cultures transferred from sulfate to methionine medium while having negligible effect on antibiotic synthesis. Mutant cultures of C. acremonium, with an increased potential to synthesize sulfur-containing antibiotics, have decreased ability to degrade methionine for other cellular requirements and sulfatase derepression is proportionately increased. The sulfatase is thus regulated by the biosynthesis of cephalosporin C and penicillin N at the expense of sulfur-containing compounds required for other cellular processes.

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