scholarly journals Studies on lytic enzyme toward cariogenic streptococci. Part VI. Purification and characterization of N-acetylmuramyl-L-alanine amidase from Streptomyces globisporus 1829.

1984 ◽  
Vol 48 (2) ◽  
pp. 261-269 ◽  
Author(s):  
Shigeo KAWATA ◽  
Tadashi TAKEMURA ◽  
Yoshiyuki TAKASE ◽  
Kanae YOKOGAWA
Keyword(s):  
Lytic Enzyme ◽  
Purification And Characterization

Purification and Characterization of the Lytic Enzyme Produced byStreptomyces rutgersensisH-46

1981 ◽  
Vol 45 (10) ◽  
pp. 2289-2300
Author(s):  
Kiyoshi Hayashi ◽  
Takafumi Kasumi ◽  
Naoya Kubo ◽  
Nobuzo Tsumura
Keyword(s):  
Lytic Enzyme ◽  
Purification And Characterization

Purification and characterization of cell wall lytic enzyme released by mating gametes of Chlamydomonas reinhardtii

FEBS Letters ◽  
1984 ◽  
Vol 166 (2) ◽  
pp. 293-297 ◽  
Author(s):  
Yoshihiro Matsuda ◽  
Atsuko Yamasaki ◽  
Saito Tatsuaki ◽  
Tetsuya Yamaguchi
Keyword(s):  
Cell Wall ◽  
Chlamydomonas Reinhardtii ◽  
Lytic Enzyme ◽  
Purification And Characterization

Purification and characterization of phosphoglucomutase from peas

1994 ◽  
Vol 92 (3) ◽  
pp. 479-486 ◽  
Author(s):  
Cynthia M. Galloway ◽  
W. Mack Dugger
Keyword(s):  
Purification And Characterization

Purification and Characterization of Tissue-Type Plasminogen Activator in Maxillary Mucosa with Chronic Inflammation

1985 ◽  
Vol 54 (02) ◽  
pp. 485-489 ◽  
Author(s):  
Yukiyoshi Hamaguchi ◽  
Masuichi Ohi ◽  
Yasuo Sakakura ◽  
Yasuro Miyoshi
Keyword(s):  
Plasminogen Activator ◽  
Chronic Inflammation ◽  
Gel Filtration ◽  
Potassium Acetate ◽  
Tissue Type ◽  
Purification And Characterization ◽  
Tissue Type Plasminogen Activator ◽  
Urokinase Inhibitor ◽  
Type Plasminogen Activator

SummaryTissue-type plasminogen activator (TPA) was purified from maxillary mucosa with chronic inflammation and compared with urokinase. Purification procedure consisted of the extraction from delipidated mucosa with 0.3M potassium acetate buffer (pH 4.2), 66% saturation of ammonium sulfate, zinc chelate-Sepharose, concanavalin A-Sepharose and Sephadex G-100 gel filtration chromatographies.The molecular weight of the TPA was approximately 58,000 ± 3,000. Its activity was enhanced in the presence of fibrin and was quenched by placental urokinase inhibitor, but not quenched by anti-urokinase antibody. The TPA made no precipitin line against anti-urokinase antibody, while urokinase did.All these findings indicate that the TPA in maxillary mucosa with chronic inflammation is immunologically dissimilar to urokinase and in its affinity for fibrin.

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