A plate culture method for the simultaneous detection of bacteria producing pullulan- and/or starch-hydrolyzing enzymes.

Abstract Background: This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis (EPTB). Methods: From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including EPTB (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing (SAT) method. The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of EPTB. Results: For 433 EPTB specimens and 49 non-TB specimens, the simultaneous amplification and testing tuberculosis (SAT-TB) results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6%, 79.4%, 59.4%, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6% and 100%, respectively, the cultures test were 29.3% and 98.0%. Conclusions: SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.

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Application of Real-Time Simultaneous Amplification and Testing Method to Accurately and Rapidly Detect Extra-pulmonary Tuberculosis

10.21203/rs.2.22566/v2 ◽

2020 ◽

Author(s):

Tongxin Li ◽

Tao Shi ◽

Ying Sun ◽

Kan Zhou ◽

Zhenggu Huang ◽

...

Keyword(s):

Pulmonary Tuberculosis ◽

Clinical Diagnosis ◽

Sensitivity And Specificity ◽

Simultaneous Detection ◽

Culture Method ◽

Culture Methods ◽

Predictive Values ◽

Extra Pulmonary Tuberculosis ◽

Testing Method ◽

Extra Pulmonary

Abstract Background: This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis. Methods: From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including extra-pulmonary tuberculosis (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing method (SAT). The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of extra-pulmonary tuberculosis. Results: For 433 extra-pulmonary tuberculosis (EPTB) specimens and 49 non-TB specimens, the SAT-TB results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6%, 79.4%, 59.4%, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6% and 100%, respectively, the cultures test were 29.3% and 98.0%. Conclusions: SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.

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Starch Hydrolyzing Enzymes for Retarding the Staling of Rice Bread

Cereal Chemistry ◽

10.1094/cchem.2003.80.6.750 ◽

2003 ◽

Vol 80 (6) ◽

pp. 750-754 ◽

Cited By ~ 86

Author(s):

Hardeep Singh Gujral ◽

Mónica Haros ◽

Cristina M. Rosell

Keyword(s):

Hydrolyzing Enzymes ◽

Starch Hydrolyzing Enzymes

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Changes in Activities of Starch- hydrolyzing Enzymes in Roots of Sugar Beets

Agricultural and Biological Chemistry ◽

10.1080/00021369.1987.10868404 ◽

1987 ◽

Vol 51 (9) ◽

pp. 2583-2584

Author(s):

Hiroshi Masuda ◽

Toshimasa Takahashi ◽

Shiro Sugawara

Keyword(s):

Sugar Beets ◽

Hydrolyzing Enzymes ◽

Starch Hydrolyzing Enzymes

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Changes in activities of starch-hydrolyzing enzymes in roots of sugar beets.

Agricultural and Biological Chemistry ◽

10.1271/bbb1961.51.2583 ◽

1987 ◽

Vol 51 (9) ◽

pp. 2583-2584

Author(s):

Hiroshi MASUDA ◽

Toshimasa TAKAHASHI ◽

Shiro SUGAWARA

Keyword(s):

Sugar Beets ◽

Hydrolyzing Enzymes ◽

Starch Hydrolyzing Enzymes

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Purification and characterization of a novel α-glucosidase from an Antarctic yeast Dioszegia fristingensis isolate

Amylase ◽

10.1515/amylase-2017-0005 ◽

2017 ◽

Vol 1 (1) ◽

Cited By ~ 2

Author(s):

Mario Carrasco ◽

Jennifer Alcaíno ◽

Víctor Cifuentes ◽

Marcelo Baeza

Keyword(s):

Industrial Processes ◽

High Catalytic Activity ◽

Purification And Characterization ◽

Amylolytic Enzyme ◽

Commercial Enzymes ◽

Antarctic Yeast ◽

Hydrolyzing Enzymes ◽

Starch Hydrolyzing Enzymes ◽

A Current

AbstractStarch hydrolyzing enzymes, amylases, are important commercial enzymes used in several productive areas. A current tendency is to find amylases with high catalytic activity at 20-40°C, to generate products that work well at low temperatures, such as detergents, and for energy saving resources in industrial processes. In this work, an α-glucosidase secreted by the cold-adapted yeast Dioszegia fristingensis was purified and biochemically characterized. The effect of physicochemical parameters on the enzyme activity was evaluated. According to our results, the amylolytic enzyme secreted by D. fristingensis is a monomeric α-glucosidase of about 30 kDa that displayed the highest activity at 37-40°C and at pH 5.5-6.5,in the presence of 10 mM CaCl

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Sugar Profile of Syrups from Malted and Unmalted Rice of Different Varieties

Journal of Food Research ◽

10.5539/jfr.v8n1p52 ◽

2019 ◽

Vol 8 (1) ◽

pp. 52 ◽

Cited By ~ 1

Author(s):

Chigozie E. Ofoedu ◽

Chijioke M. Osuji ◽

Moses Ojukwu

Keyword(s):

Raw Material ◽

Alpha Amylase ◽

Sugar Profile ◽

Hydrolyzing Enzymes ◽

Maltose Syrup ◽

Starch Hydrolyzing Enzymes

Rice syrup was produced from ten varieties of locally available rice in Nigeria. Flours of malted and unmalted rice from different varieties were treated with a combination of starch hydrolyzing enzymes (Amyloglucosidase, Bacterial α-amylase and Fungal α-amylase); and the starch hydrolysates were either filtered and/or centrifuged at the end of hydrolysis. The resulting rice syrup was evaluated for sugar compositions (maltose, glucose, maltotriose, sucrose, raffinose and stachyose) using HPLC. The results showed that syrups from malted rice had significantly higher (p<0.05) maltose and maltotriose concentration than syrups from unmalted rice. The resultant syrup is a ‘High Maltose Syrup’ since maltose was found to be the predominant sugar in the rice syrup with concentration of above 50% especially for malted samples. Rice syrup can be widely applied as a potential raw material in beverage and confectionery industries as well as a good adjunct for brewing since the sugar profile of the rice syrup was similar to that of barley wort.

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Application of Real-Time Simultaneous Amplification and Testing Method to Accurately and Rapidly Detect Extra-pulmonary Tuberculosis

10.21203/rs.2.22566/v1 ◽

2020 ◽

Author(s):

Tongxin Li ◽

Tao Shi ◽

Ying Sun ◽

Kan Zhou ◽

Zhenggu Huang ◽

...

Keyword(s):

Pulmonary Tuberculosis ◽

Clinical Diagnosis ◽

Sensitivity And Specificity ◽

Simultaneous Detection ◽

Culture Method ◽

Culture Methods ◽

Predictive Values ◽

Extra Pulmonary Tuberculosis ◽

Testing Method ◽

Extra Pulmonary

Abstract Background: This study aimed to establish and evaluate a simultaneous amplification and testing method for detection of extra-pulmonary tuberculosis. Methods: From January 2016 and December 2017 the pus or surgical excision from the lesions of inpatients admitted from Chongqing Public Health Treatment Center were collected. According to the clinical diagnosis, the samples were divided into two groups including extra-pulmonary tuberculosis (Group A) and other diseases excluded from tuberculosis diseases (Group B). Simultaneous detection of Mycobacterium tuberculosis (MTB) used Roche culture method, liquid culture method and simultaneous amplification and testing method (SAT). The sensitivity and specificity of the SAT method were compared with culture methods and clinical diagnosis of extra-pulmonary tuberculosis. Results: For 433 extra-pulmonary tuberculosis (EPTB) specimens and 49 non-TB specimens, the SAT-TB results correlated with 80.5% (388/482 specimens) of the culture assay results. The sensitivity, specificity, and positive and negative predictive values of the SAT-TB test for the diagnosis of EPTB were 83.6%, 79.4%, 59.4%, and 93.0%, respectively, compared to culture methods. Compared with the clinical diagnosis of patients, the sensitivity and specificity of the SAT-TB test were 41.6% and 100%, respectively, the cultures test were 29.3% and 98.0%. Conclusions: SAT test is a simple and rapid test with high specificity which may enhance the detection of EPTB. SAT-TB is a higher clinical diagnosis value for EPTB in clinical microbiology laboratories.

Download Full-text