Bisphenol A (BPA) has been considered as a weak environmental estrogen, as similar to estradiol that has a potential in stimulating some cellular responses and phenotype changes. In addition, the ecological impacts of BPA to aquatic organisms have been increasingly raised at environmental relevant concentrations that potentially may affect to human health at early–life. This study used 3-day old zebrafish larvae (Danio rerio) as a model for toxicological testing. The semistatic testing was conducted to investigate the effects of different BPA concentrations (5 mg/L, 6 mg/L, 7 mg/L, 8 mg/L and 9 mg/L) that induced morphorlogical and physiological changes during the early development. As the results, the LC50-24hrs, LC50-48hrs, LC50-72hrs and LC50-96hrs were determined as 9.503 mg/L, 8.688 mg/L, 7.328 mg/L and 6.669 mg/L, respectively. Phenotypic analysis revealed that toxicity caused cardiac edema. The result obtained from this research provided relevant information for environmental and human risk assessments.