Recombinant interferon-gamma secreted by CHO-320 cells cultivated in suspension in protein-free media is protected against extracellular proteolysis by the expression of natural protease inhibitors and by the addition of plant protein hydrolysates to the culture medium

2005 ◽  
Author(s):  
Johann Mols ◽  
Chantal Peeters-Joris ◽  
Ruddy Wattiez ◽  
Spiros Agathos ◽  
Yves-Jacques SCHNEIDER
Keyword(s):  
Protease Inhibitors ◽  
Interferon Gamma ◽  
Culture Medium ◽  
Protein Hydrolysates ◽  
Plant Protein ◽  
Recombinant Interferon ◽  
Free Media ◽  
Extracellular Proteolysis

scholarly journals Plant protein hydrolysates support CHO-320 cells proliferation and recombinant IFN-γ production in suspension and inside microcarriers in protein-free media

2004 ◽  
Vol 44 (3) ◽  
pp. 103-114 ◽  
Author(s):  
J. S. Ballez ◽  
J. Mols ◽  
C. Burteau ◽  
S. N. Agathos ◽  
Y. J. Schneider
Keyword(s):  
Protein Hydrolysates ◽  
Plant Protein ◽  
Ifn Γ ◽  
Free Media

Plant protein hydrolysates (plant peptones) as substitutes for animal proteins in embryo culture medium

2009 ◽  
Vol 21 (4) ◽  
pp. 587 ◽  
Author(s):  
F. George ◽  
D. Kerschen ◽  
A. Van Nuffel ◽  
J. F. Rees ◽  
I. Donnay
Keyword(s):  
Culture Medium ◽  
Similar Rate ◽  
Protein Hydrolysates ◽  
Plant Protein ◽  
Fluid Medium ◽  
Anti Oxidant ◽  
The Cost ◽  
Oviduct Fluid

The aim of the present study was to improve the sanitary quality of in vitro-produced bovine embryos by using plant protein hydrolysates (plant peptones) as substitutes for animal proteins. Peptones were compared with bovine serum albumin (BSA) as the protein source in synthetic oviduct fluid medium and the quality of the resulting embryos was determined. Two batches of peptones (wheat and cotton) were selected on the basis of their anti-oxidant properties. When added to the culture medium, both peptones (at 0.56 mg mL–1 for cotton peptone and at 0.18 mg mL–1 for wheat peptone) led to similar developmental and hatching rates compared with 4 mg mL–1 BSA and embryos were equally resistant to freezing and able to elongate after transfer. Surprisingly, a significant decrease in reduced glutathione (GSH) content was observed when embryos were produced with plant peptone instead of BSA. Supplementation of the culture medium with precursors of GSH (cysteine and β-mercaptoethanol) significantly increased the GSH content. A shift of the sex ratio towards male embryos was seen for Day 8 embryos cultured with wheat peptone, whereas no shift was observed for embryos cultured in the presence of BSA or polyvinylpyrrolidone. In conclusion, culture with plant peptones enables embryos to be obtained at a similar rate and of similar quality to that seen following the use of BSA. The use of the plant peptones increased the sanitary quality of the embryos and decreased the cost of embryo production.

Use of plant protein hydrolysates for varicella virus production in Serum-Free Medium

2005 ◽  
Vol 27 (4) ◽  
pp. 243-248 ◽  
Author(s):  
Bok-Hwan Chun ◽  
Yong Kwon Lee ◽  
Won-Gi Bang ◽  
Namhyun Chung
Keyword(s):  
Virus Production ◽  
Protein Hydrolysates ◽  
Plant Protein ◽  
Serum Free Medium ◽  
Free Medium ◽  
Serum Free ◽  
Varicella Virus

Recognition of recombinant interferon-gamma from Felidae species by anti-cat antibodies

2021 ◽  
pp. 110327
Author(s):  
Jordan L. Mitchell ◽  
Anna Raper ◽  
Danièlle A. Gunn-Moore ◽  
Jayne C. Hope
Keyword(s):  
Interferon Gamma ◽  
Recombinant Interferon
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