RECOMBINANT INTERFERON-γ SECRETED BY CHINESE HAMSTER OVARY-320 CELLS CULTIVATED IN SUSPENSION IN PROTEIN-FREE MEDIA IS PROTECTED AGAINST EXTRACELLULAR PROTEOLYSIS BY THE EXPRESSION OF NATURAL PROTEASE INHIBITORS AND BY THE ADDITION OF PLANT PROTEIN HYDROLYSATES TO THE CULTURE MEDIUM

2005 ◽  
Vol 41 (3) ◽  
pp. 83 ◽  
Author(s):  
J. MOLS ◽  
C. PEETERS-JORIS ◽  
R. WATTIEZ ◽  
S. N. AGATHOS ◽  
Y.-J. SCHNEIDER
Keyword(s):  
Protease Inhibitors ◽  
Chinese Hamster Ovary ◽  
Culture Medium ◽  
Chinese Hamster ◽  
Interferon Γ ◽  
Protein Hydrolysates ◽  
Plant Protein ◽  
Recombinant Interferon ◽  
Free Media ◽  
Extracellular Proteolysis

Metabolic Effects on Recombinant Interferon-γ Glycosylation in Continuous Culture of Chinese Hamster Ovary Cells

2000 ◽  
Vol 44 (5) ◽  
pp. 770-783
Author(s):  
Gregg B. Nyberg ◽  
R. Robert Balcarcel ◽  
Brian D. Follstad ◽  
Gregory Stephanopoulos ◽  
Daniel I. C. Wang
Keyword(s):  
Continuous Culture ◽  
Chinese Hamster Ovary ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Interferon Γ ◽  
Metabolic Effects ◽  
Recombinant Interferon ◽  
Ovary Cells

Production of Recombinant Proteins in Chinese Hamster Ovary Cells Using A Protein-Free Cell Culture Medium

1995 ◽  
Vol 13 (4) ◽  
pp. 389-392 ◽  
Author(s):  
Michael Zang ◽  
Helmut Trautmann ◽  
Christine Gandor ◽  
Ferruccio Messi ◽  
Fred Asselbergs ◽  
...  
Keyword(s):  
Cell Culture ◽  
Recombinant Proteins ◽  
Chinese Hamster Ovary ◽  
Culture Medium ◽  
Chinese Hamster Ovary Cells ◽  
Chinese Hamster ◽  
Free Cell ◽  
Cell Culture Medium ◽  
Ovary Cells

scholarly journals Hydrophobic glycosides of N-acetylglucosamine can act as primers for polylactosamine synthesis and can affect glycolipid synthesis in vivo

1995 ◽  
Vol 307 (3) ◽  
pp. 791-797 ◽  
Author(s):  
D C A Neville ◽  
R A Field ◽  
M A J Ferguson
Keyword(s):  
Chinese Hamster Ovary ◽  
Cho Cells ◽  
Culture Medium ◽  
Linear Array ◽  
Chinese Hamster ◽  
Living Cells ◽  
Anomeric Configuration ◽  
Glycolipid Synthesis ◽  
Synthetic Capacity

Several hydrophobic glycosides of N-acetylglucosamine (GlcNAc) served as primers for polylactosamine synthesis when added to Chinese hamster ovary (CHO) cells. The modified glycosides, containing one to six lactosamine repeats in linear array, were sialylated and secreted into the culture medium. The relative efficiencies of the glycosides to serve as primers were dependent on the nature of the aglycone and on the anomeric configuration of the GlcNAc residue. The same compounds were tested for their effects on glycolipid synthesis in CHO cells. All of the beta-glycosides significantly inhibited the synthesis of the lactoseries glycolipid GM3 whereas the alpha-glycoside was inactive. The compound GlcNAc alpha 1-O-benzyl- was the most efficient primer of polylactosamine synthesis and had no effect on glycolipid synthesis. This compound may have potential for the assay of the polylactosamine synthetic capacity of living cells.

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