scholarly journals Expression and Purification of Recombinant Marek's Disease Virus Serotype 1 Specific Phosphorylated Protein pp38 in E. coli.

1994 ◽  
Vol 56 (5) ◽  
pp. 823-826 ◽  
Author(s):  
Daiji ENDOH ◽  
Masahiro NIIKURA ◽  
Kanji HIRAI ◽  
Naohito INAGAKI ◽  
Masanobu HAYASHI ◽  
...  
Keyword(s):  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Expression And Purification ◽  
E Coli ◽  
Phosphorylated Protein ◽  
Virus Serotype ◽  
Serotype 1

scholarly journals Quantitative profiling of the shedding rate of the three Marek's disease virus (MDV) serotypes reveals that challenge with virulent MDV markedly increases shedding of vaccinal viruses

2007 ◽  
Vol 88 (8) ◽  
pp. 2121-2128 ◽  
Author(s):  
Aminul Islam ◽  
Stephen W. Walkden-Brown
Keyword(s):  
Disease Virus ◽  
Broiler Chickens ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Commercial Broiler ◽  
Virulent Virus

The shedding profile of Marek's disease virus serotype 1 (MDV1, virulent), serotype 2 (MDV2, vaccinal) and herpesvirus of turkeys (HVT, vaccinal) in commercial broiler chickens was determined by measuring the daily rate of production of feather dander from chickens housed in isolators and by quantifying the viral load of each of these serotypes in the dander using quantitative real-time PCR (qPCR). MDV1 and HVT viruses were detectable in dander filtered from isolator exhaust air from day 7 and MDV2 from day 12 after infection and thereafter until the end of the experiment at 61 days of age of the chickens. There was no difference in shedding rate among the three MDV1 isolates. Daily shedding of MDV1 increased sharply between days 7 and 28 and stabilized thereafter at about 109 virus copies per chicken per day, irrespective of vaccination status. Challenge with the three different MDV1 isolates markedly increased shedding of the vaccinal viruses HVT and MDV2 in dander by 38- and 75-fold, respectively. These results demonstrate the utility of qPCR for the differentiation and quantification of different MDV serotypes in feather dander and have significant implications for the routine monitoring of Marek's disease using qPCR assays of dust, for epidemiological modelling of the behaviour and spread of MDVs in chicken populations and for studies into the evolution of virulence in MDV1 in the face of blanket vaccination with imperfect vaccines that ameliorate disease but do not prevent infection and replication of virulent virus.

scholarly journals Pathotyping of recent Indian field isolates of Marek's disease virus serotype 1

2015 ◽  
Vol 59 (02) ◽  
pp. 156-165 ◽  
Author(s):  
P. SURESH ◽  
J. JOHNSON RAJESWAR ◽  
K. SUKUMAR ◽  
T. J. HARIKRISHNAN ◽  
P. SRINIVASAN
Keyword(s):  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Field Isolates ◽  
Virus Serotype ◽  
Serotype 1

Preparation of monoclonal antibodies against Marek's disease virus serotype 1 glycoprotein D expressed by a recombinant baculovirus

1995 ◽  
Vol 38 (2-3) ◽  
pp. 219-230 ◽  
Author(s):  
Mitsuru Ono ◽  
Hyung-Kwan Jang ◽  
Ken Maeda ◽  
Yasushi Kawaguchi ◽  
Yukinobu Tohya ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Disease Virus ◽  
Recombinant Baculovirus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Glycoprotein D ◽  
Virus Serotype ◽  
Serotype 1

scholarly journals Difference in the meq Gene between Oncogenic and Attenuated Strains of Marek's Disease Virus Serotype 1.

2000 ◽  
Vol 62 (3) ◽  
pp. 287-292 ◽  
Author(s):  
Sung-Il LEE ◽  
Michihiro TAKAGI ◽  
Kazuhiko OHASHI ◽  
Chihiro SUGIMOTO ◽  
Misao ONUMA
Keyword(s):  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Serotype ◽  
Serotype 1 ◽  
Meq Gene

scholarly journals Characterization of Marek's Disease Virus Serotype 1 (MDV-1) Deletion Mutants That Lack UL46 to UL49 Genes: MDV-1 UL49, Encoding VP22, Is Indispensable for Virus Growth

2002 ◽  
Vol 76 (4) ◽  
pp. 1959-1970 ◽  
Author(s):  
Fabien Dorange ◽  
B. Karsten Tischer ◽  
Jean-François Vautherot ◽  
Nikolaus Osterrieder
Keyword(s):  
Amino Acids ◽  
Disease Virus ◽  
Marek's Disease Virus ◽  
Marek's Disease ◽  
Homologous Gene ◽  
Marek’S Disease Virus ◽  
Marek’S Disease ◽  
Virus Growth ◽  
Virus Serotype ◽  
Serotype 1

ABSTRACT Experiments were conducted to investigate the roles of Marek's disease virus serotype 1 (MDV-1) major tegument proteins VP11/12, VP13/14, VP16, and VP22 in viral growth in cultured cells. Based on a bacterial artificial chromosome clone of MDV-1 (BAC20), mutant viruses were constructed in which the MDV-1 homologs of UL46, UL47, UL48, or UL49 were deleted alone and in various combinations. It could be demonstrated that the UL46, UL47, and UL48 genes are dispensable for MDV-1 growth in chicken embryonic skin and quail muscle QM7 cells, although the generated virus mutants exhibited reduced plaque sizes in all cell types investigated. In contrast, a UL49-negative MDV-1 (20Δ49) and a UL48-UL49 (20Δ48-49) doubly negative mutant were not able to produce MDV-1-specific plaques on either cell type. It was confirmed that this growth restriction is dependent on the absence of VP22 expression, because growth of these mutant viruses could be partially restored on cells that were cotransfected with a UL49 expression plasmid. In addition, we were able to demonstrate that cell-to-cell spread of MDV-1 conferred by VP22 is dependent on the expression of amino acids 37 to 187 of MDV-1 VP22, because expression plasmids containing MDV-1 UL49 mutant genes with deletions of amino acids 1 to 37 or 188 to 250 were still able to restore partial growth of the 20Δ49 and 20Δ48-49 viruses. These results demonstrate for the first time that an alphaherpesvirus UL49-homologous gene is essential for virus growth in cell culture.

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