A depressurization accident is a design-basis accidents of a very high temperature reactor. When a depressurization accident occurs, air is expected to enter the reactor pressure vessel from the breach and oxidize in-core graphite structures. Therefore, it is important to know a mixing process of different kind of gases in the stable and unstable stratified fluid layer. Especially, it is also important to examine an influence of localized natural convection and molecular diffusion on mixing process from a viewpoint of safety. In order to predict and analyze the phenomena of air ingress during a depressurization accident, therefore, it is important to develop the method for prevention of air ingress during the accident. We have carried out an experiment and a numerical analysis using three-dimensional computational fluid dynamics (3D CFD) to obtain the mixing process of two component gases and flow characteristics of the localized natural convection. This study is also to investigate a control method of natural circulation of air by injection of helium gas. The numerical model consists of a storage tank and a reverse U-shaped vertical slot. They are separated by a partition plate. One side of the left wall of the left side vertical slot is heated and the other side was cooled. The right side vertical slot is cooled. The procedure of the experiment and the numerical analysis is as follows. Firstly, the storage tank was filled with heavy gas and the reverse U-shaped vertical slot was filled with light gas. In the left side vertical slot, the localized natural convection was generated by the temperature difference between the vertical walls. The flow characteristics were obtained by the experiment and steady state analysis. The unsteady state experiment and analysis were started after the partition plate was opened. The result obtained in the experiment was simulated by the numerical analysis quantitatively. The gases were mixed by molecular diffusion and natural convection. After the time elapsed, natural circulation occurred. When the temperature difference of the left vertical fluid layer was set to 100K and the combination of the mixed gas was helium and nitrogen, natural circulation produced after 110 minutes elapsed.