The present study deals with the application of the Magneto-Rheological (MR) fluid assisted semi-active damper as a replacement to the conventional suspension system in Maruti 800 car (source vehicle). MR fluid damper is designed, fabricated and automated with a microcontroller. This experimentation is carried out with real time instrumentation on the selected road profile as a vehicle dynamic approach. Results obtained from the travel imply that MR fluid suspension suppresses the vibrations more effectively than the existing passive damper system. The MR fluid dampens the acceleration and displacement of the piston to a greater extent thereby controlling the ride comfort.