scholarly journals Numerical Simulation of Various Shape Changes of a Swollen Red Blood Cell by Decrease of Its Volume.

2003 ◽  
Vol 69 (677) ◽  
pp. 14-21 ◽  
Author(s):  
Shigeo WADA ◽  
Ryo KOBAYASHI
Keyword(s):  
Numerical Simulation ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Shape Changes

scholarly journals Effect of red blood cell shape changes on haemoglobin interactions and dynamics: a neutron scattering study

2020 ◽  
Vol 7 (10) ◽  
pp. 201507
Author(s):  
Keyun Shou ◽  
Mona Sarter ◽  
Nicolas R. de Souza ◽  
Liliana de Campo ◽  
Andrew E. Whitten ◽  
...  
Keyword(s):  
Blood Cell ◽  
Neutron Scattering ◽  
Protein Interactions ◽  
Red Blood Cell ◽  
Small Angle ◽  
Time Scale ◽  
Small Angle Neutron Scattering ◽  
Length Scale ◽  
Elastic Neutron ◽  
Shape Changes

By using a combination of experimental neutron scattering techniques, it is possible to obtain a statistical perspective on red blood cell (RBC) shape in suspensions, and the inter-relationship with protein interactions and dynamics inside the confinement of the cell membrane. In this study, we examined the ultrastructure of RBC and protein–protein interactions of haemoglobin (Hb) in them using ultra-small-angle neutron scattering and small-angle neutron scattering (SANS). In addition, we used the neutron backscattering method to access Hb motion on the ns time scale and Å length scale. Quasi-elastic neutron scattering (QENS) experiments were performed to measure diffusive motion of Hb in RBCs and in an RBC lysate. By using QENS, we probed both internal Hb dynamics and global protein diffusion, on the accessible time scale and length scale by QENS. Shape changes of RBCs and variation of intracellular Hb concentration were induced by addition of the Na + -selective ionophore monensin and the K + -selective one, valinomycin. The experimental SANS and QENS results are discussed within the framework of crowded protein solutions, where free motion of Hb is obstructed by mutual interactions.

scholarly journals Curling and Local Shape Changes of Red Blood Cell Membranes Driven by Cytoskeletal Reorganization

2010 ◽  
Vol 99 (3) ◽  
pp. 808-816 ◽  
Author(s):  
Doron Kabaso ◽  
Roie Shlomovitz ◽  
Thorsten Auth ◽  
Virgilio L. Lew ◽  
Nir S. Gov
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Cell Membranes ◽  
Cytoskeletal Reorganization ◽  
Local Shape ◽  
Red Blood Cell Membranes ◽  
Shape Changes

scholarly journals Surface model of the human red blood cell simulating changes in membrane curvature under strain

2021 ◽  
Author(s):  
Philip W. Kuchel ◽  
Charles D. Cox ◽  
Daniel Daners ◽  
Dmitry Shishmarev ◽  
Petrik Galvosas
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Morphological Changes ◽  
Three Dimensional ◽  
Membrane Curvature ◽  
Glycolytic Flux ◽  
Surface Model ◽  
Principal Curvatures ◽  
Quartic Equation ◽  
Shape Changes

Abstract The highly deformable red blood cell (erythrocyte; RBC) responds to mechanically imposed shape changes with enhanced glycolytic flux and cation transport. Such morphological changes are produced experimentally by suspending the cells in a gelatin gel, which is then elongated or compressed in a special apparatus inside an NMR spectrometer. However, direct mathematical predictions of the shapes of the morphed cells have not been reported before. We used recently available functions in Mathematica to triangularize and then compute four types of curvature. The RBCs were described by a previously presented quartic equation in three dimensional (3D) Cartesian space. A key finding was the extent to which the maximum and minimum Principal Curvatures were localized symmetrically in patches at the poles or equators and distributed in rings around the main axis of the strained RBC. The simulations, on the nano-metre to micro-meter scale of curvature, suggest activation of only a subset of the intrinsic mechanosensitive cation channels, Piezo1, during experiments carried out with controlled distortions that persist for many hours. This view is consistent with a recent proposal for non-uniform distribution of Piezo1 molecules around the RBC membrane. On the other hand, if the curvature that gates Piezo1 is at a much finer length scale, then membrane tension will determine local curvature and micron scale curvature as described here will be less likely to influence Piezo1 activity. The geometrical reorganization of the simulated cytoskeleton helps understanding of the concerted metabolic and cation-flux responses of the RBC to mechanically imposed shape changes.

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