Low-Intensity Pulsed Ultrasound Accelerates Rat Femoral Fracture Healing by Acting on the Various Cellular Reactions in the Fracture Callus

2001 ◽  
Vol 16 (4) ◽  
pp. 671-680 ◽  
Author(s):  
Yoshiaki Azuma ◽  
Masaya Ito ◽  
Yoshifumi Harada ◽  
Hideko Takagi ◽  
Tomohiro Ohta ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Femoral Fracture ◽  
Pulsed Ultrasound ◽  
Fracture Callus ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Osteocytes as main responders to low-intensity pulsed ultrasound treatment during fracture healing

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Tatsuya Shimizu ◽  
Naomasa Fujita ◽  
Kiyomi Tsuji-Tamura ◽  
Yoshimasa Kitagawa ◽  
Toshiaki Fujisawa ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Transcriptional Control ◽  
Target Genes ◽  
Pulsed Ultrasound ◽  
Activated T Cells ◽  
In Vivo Models ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Ultrasound Stimulation

AbstractUltrasound stimulation is a type of mechanical stress, and low-intensity pulsed ultrasound (LIPUS) devices have been used clinically to promote fracture healing. However, it remains unclear which skeletal cells, in particular osteocytes or osteoblasts, primarily respond to LIPUS stimulation and how they contribute to fracture healing. To examine this, we utilized medaka, whose bone lacks osteocytes, and zebrafish, whose bone has osteocytes, as in vivo models. Fracture healing was accelerated by ultrasound stimulation in zebrafish, but not in medaka. To examine the molecular events induced by LIPUS stimulation in osteocytes, we performed RNA sequencing of a murine osteocytic cell line exposed to LIPUS. 179 genes reacted to LIPUS stimulation, and functional cluster analysis identified among them several molecular signatures related to immunity, secretion, and transcription. Notably, most of the isolated transcription-related genes were also modulated by LIPUS in vivo in zebrafish. However, expression levels of early growth response protein 1 and 2 (Egr1, 2), JunB, forkhead box Q1 (FoxQ1), and nuclear factor of activated T cells c1 (NFATc1) were not altered by LIPUS in medaka, suggesting that these genes are key transcriptional regulators of LIPUS-dependent fracture healing via osteocytes. We therefore show that bone-embedded osteocytes are necessary for LIPUS-induced promotion of fracture healing via transcriptional control of target genes, which presumably activates neighboring cells involved in fracture healing processes.

Low-intensity pulsed ultrasound affects RUNX2 immunopositive osteogenic cells in delayed clinical fracture healing

Bone ◽  
2009 ◽  
Vol 45 (5) ◽  
pp. 862-869 ◽  
Author(s):  
Sjoerd Rutten ◽  
Peter A. Nolte ◽  
Clara M. Korstjens ◽  
Jenneke Klein-Nulend
Keyword(s):  
Fracture Healing ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Osteogenic Cells ◽  
Low Intensity ◽  
Clinical Fracture

scholarly journals Fracture Nonunion Treated with Low-Intensity Pulsed Ultrasound and Monitored with Ultrasonography: A Feasibility Study

2021 ◽  
Vol 2021 ◽  
pp. 1-5
Author(s):  
Peizhen Zhang ◽  
Pengdong Li ◽  
Shihai Liao ◽  
Xuan Li ◽  
Wufan Chen ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Bone Fracture ◽  
Soft Tissues ◽  
Callus Formation ◽  
Distal Tibia ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Cord Injury ◽  
Fracture Nonunion

The positive effect of low-intensity pulsed ultrasound (LIPUS) on bone fracture healing has been proved. However, during the period of LIPUS therapy, it is undetermined whether LIPUS promotes the formation of heterotopic ossification (HO), which usually occurs in muscle tissues after trauma such as bone fracture and spinal cord injury. Here, we used 6-week LIPUS therapy in a 42-year-old Chinese male patient with a fracture nonunion in combination with ultrasonography for monitoring fracture healing and HO formation. After the LIPUS therapy, the mineralized bone formation in the area of defect of the distal tibia was presented in an ultrasound image, which was consistent with the outcome of plain radiography showing callus formation and the blurred fracture line in the area exposed to LIPUS. In addition, ultrasound images revealed no evidence of HO development within soft tissues during the period of LIPUS therapy. This study suggests that ultrasonography is a potential tool to guarantee the performance of LIPUS therapy with monitoring HO formation. Easy to use, the integration of the handheld ultrasound scanner and the ultrasonic therapeutic apparatus is entirely dedicated to help orthopedists make high-quality care and diagnosis.

Low‐Intensity Pulsed Ultrasound Accelerates Traumatic Vertebral Fracture Healing by Coupling Proliferation of Type H Microvessels

2018 ◽  
Vol 37 (7) ◽  
pp. 1733-1742 ◽  
Author(s):  
Suiyi Wu ◽  
Ximing Xu ◽  
Jingchuan Sun ◽  
Yao Zhang ◽  
Jiangang Shi ◽  
...  
Keyword(s):  
Vertebral Fracture ◽  
Fracture Healing ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Low-intensity pulsed ultrasound accelerates fracture healing by stimulation of recruitment of both local and circulating osteogenic progenitors

2012 ◽  
Vol 30 (9) ◽  
pp. 1516-1521 ◽  
Author(s):  
Ken Kumagai ◽  
Ryohei Takeuchi ◽  
Hiroyuki Ishikawa ◽  
Yuichiro Yamaguchi ◽  
Takahiro Fujisawa ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Stimulation Of

3. Can Combined Therapy With Teriparatide and Low-Intensity Pulsed Ultrasound (LIPUS) Accelerate Fracture Healing?

2016 ◽  
Vol 30 (8) ◽  
pp. S2 ◽  
Author(s):  
Koji Nozaka ◽  
Yoichi Shimada ◽  
Naohisa Miyakoshi ◽  
Shin Yamada ◽  
Michio Hongo ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Combined Therapy ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals The effects of low-intensity pulsed ultrasound upon diabetic fracture healing

2010 ◽  
Vol 29 (2) ◽  
pp. 181-188 ◽  
Author(s):  
Michael Coords ◽  
Eric Breitbart ◽  
David Paglia ◽  
Nikolas Kappy ◽  
Ankur Gandhi ◽  
...  
Keyword(s):  
Fracture Healing ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

scholarly journals Low-intensity pulsed ultrasound increases the fracture callus strength in diabetic BB Wistar rats but does not affect cellular proliferation

2002 ◽  
Vol 20 (3) ◽  
pp. 587-592 ◽  
Author(s):  
Gregory P. Gebauer ◽  
Sheldon S. Lin ◽  
Heather A. Beam ◽  
Pedro Vieira ◽  
J. Russell Parsons
Keyword(s):  
Wistar Rats ◽  
Cellular Proliferation ◽  
Pulsed Ultrasound ◽  
Fracture Callus ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity

Poster 508 Benefits of Low-Intensity Pulsed Ultrasound Bone Stimulator for Fracture Healing in Spinal Cord Injury: A Case Report

PM&R ◽  
2012 ◽  
Vol 4 ◽  
pp. S364-S364
Author(s):  
Myrlynn Delille ◽  
Junney M. Baeza Dager ◽  
Kresimir Banovac ◽  
Luis Batlle ◽  
Karin Zachow
Keyword(s):  
Spinal Cord ◽  
Spinal Cord Injury ◽  
Case Report ◽  
Fracture Healing ◽  
Pulsed Ultrasound ◽  
Low Intensity Pulsed Ultrasound ◽  
Low Intensity ◽  
Cord Injury ◽  
Bone Stimulator ◽  
Ultrasound Bone
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