Three-dimensional display through speckle patterns: a single exposure method

1988 ◽  
Vol 27 (12) ◽  
pp. 2370 ◽  
Author(s):  
Marcelo Trivi ◽  
Nestor Bolognini
Author(s):  
Neng-Yu Zhang ◽  
Terence Wagenknecht ◽  
Michael Radermacher ◽  
Tom Obrig ◽  
Joachim Frank

We have reconstructed the 40S ribosomal subunit at a resolution of 4 nm using the single-exposure pseudo-conical reconstruction method of Radermacher et al.Small (40S) ribosomal subunits were Isolated from rabbit reticulocytes, applied to grids and negatively stained (0.5% uranyl acetate) in a manner that “sandwiches” the specimen between two layers of carbon. Regions of the grid exhibiting uniform and thick staining were identified and photographed twice (magnification 49,000X). The first micrograph was always taken with the specimen tilted by 50° and the second was of the Identical area untilted (Fig. 1). For each of the micrographs the specimen was subjected to an electron dose of 2000-3000 el/nm2.Three hundred thirty particles appearing in the L view (defined in [4]) were selected from both tilted- and untilted-specimen micrographs. The untilted particles were aligned and their rotational alignment produced the azimuthal angles of the tilted particles in the conical tilt series.


Author(s):  
Honglei Xu ◽  
Linhuan Wang

In order to improve the accuracy of dynamic detection of wind field in the three-dimensional display space, system software is carried out on the actual scene and corresponding airborne radar observation information data, and the particle swarm algorithm fuzzy logic algorithm is introduced into the wind field dynamic simulation process in three-dimensional display space, to analyze the error of the filtering result in detail, to process the hurricane Lily Doppler radar measurement data with the optimal adaptive filtering according to the error data. The three-dimensional wind field synchronous measurement data obtained by filtering was compared with three-dimensional wind field synchronous measurement data of the GPS dropsonde in this experiment, the sea surface wind field measurement data of the multi-band microwave radiometer, and the wind field data at aircraft altitude.


2020 ◽  
Vol 461 ◽  
pp. 125260 ◽  
Author(s):  
Peng Sun ◽  
Chenning Tao ◽  
Jinlei Zhang ◽  
Rengmao Wu ◽  
Siqi Liu ◽  
...  

2021 ◽  
Vol 52 (S1) ◽  
pp. 613-616
Author(s):  
Gewei Yan ◽  
Jiayi Li ◽  
Chenxi Zhao ◽  
Zengxiang Lu ◽  
Zhaojun Liu

2020 ◽  
Vol 132 (22) ◽  
pp. 8494-8498 ◽  
Author(s):  
Shigang Wan ◽  
Hongqi Zhou ◽  
Jinxiong Lin ◽  
Wei Lu

2008 ◽  
Author(s):  
William J. Dallas ◽  
Hans Roehrig ◽  
Daniel J. Allen

1991 ◽  
Vol 3 (2) ◽  
pp. 194-199 ◽  
Author(s):  
Hiroshi HASHIMOTO ◽  
Satoshi YOKOYAMA ◽  
Kyoko NAKAO ◽  
Kousuke HARUKI ◽  
Motoko CHIBA ◽  
...  

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