Quantitative phase and refractive index analysis of optical fibers using differential interference contrast microscopy

2008 ◽  
Vol 47 (28) ◽  
pp. 5182 ◽  
Author(s):  
Betty Kouskousis ◽  
Daniel J. Kitcher ◽  
Stephen Collins ◽  
Ann Roberts ◽  
Greg W. Baxter
Keyword(s):  
Refractive Index ◽  
Optical Fibers ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Quantitative Phase ◽  
Index Analysis ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

Refractive index profiling of optical fibers using differential interference contrast microscopy

2005 ◽  
Vol 17 (10) ◽  
pp. 2149-2151 ◽  
Author(s):  
N.M. Dragomir ◽  
E. Ampem-Lassen ◽  
S.T. Huntington ◽  
G.W. Baxter ◽  
A. Roberts ◽  
...  
Keyword(s):  
Refractive Index ◽  
Optical Fibers ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

scholarly journals Optical sectioning in differential interference contrast microscopy

2009 ◽  
Vol 282 (16) ◽  
pp. 3223-3230 ◽  
Author(s):  
Atsushi Noguchi ◽  
Hiroshi Ishiwata ◽  
Masahide Itoh ◽  
Toyohiko Yatagai
Keyword(s):  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Optical Sectioning ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy

scholarly journals Minus-end capture of preformed kinetochore fibers contributes to spindle morphogenesis

2003 ◽  
Vol 160 (5) ◽  
pp. 671-683 ◽  
Author(s):  
Alexey Khodjakov ◽  
Lily Copenagle ◽  
Michael B. Gordon ◽  
Duane A. Compton ◽  
Tarun M. Kapoor
Keyword(s):  
Three Dimensional ◽  
Differential Interference Contrast ◽  
Differential Interference Contrast Microscopy ◽  
Spindle Formation ◽  
Capture Mechanism ◽  
Contrast Microscopy ◽  
Interference Contrast Microscopy ◽  
Kinesin Eg5 ◽  
Mitotic Kinesin Eg5 ◽  
Dependent Mechanism

Near-simultaneous three-dimensional fluorescence/differential interference contrast microscopy was used to follow the behavior of microtubules and chromosomes in living α-tubulin/GFP-expressing cells after inhibition of the mitotic kinesin Eg5 with monastrol. Kinetochore fibers (K-fibers) were frequently observed forming in association with chromosomes both during monastrol treatment and after monastrol removal. Surprisingly, these K-fibers were oriented away from, and not directly connected to, centrosomes and incorporated into the spindle by the sliding of their distal ends toward centrosomes via a NuMA-dependent mechanism. Similar preformed K-fibers were also observed during spindle formation in untreated cells. In addition, upon monastrol removal, centrosomes established a transient chromosome-free bipolar array whose orientation specified the axis along which chromosomes segregated. We propose that the capture and incorporation of preformed K-fibers complements the microtubule plus-end capture mechanism and contributes to spindle formation in vertebrates.

Sign in / Sign up

Export Citation Format

Share Document