In vivo Quantitation of Circulating Tumor Cells by High-speed Intravital Laser-scanning Confocal Microscopy

2016 ◽  
Author(s):  
Howon Seo ◽  
Yoonha Hwang ◽  
Inwon Park ◽  
Kibaek Choe ◽  
Pilhan Kim
Keyword(s):  
Confocal Microscopy ◽  
Tumor Cells ◽  
Circulating Tumor Cells ◽  
High Speed ◽  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
Scanning Confocal Microscopy

In Vivo Laser Scanning Confocal Microscopy of the Ocular Surface in Glaucoma

2014 ◽  
Vol 20 (3) ◽  
pp. 879-894 ◽  
Author(s):  
Leonardo Mastropasqua ◽  
Luca Agnifili ◽  
Rodolfo Mastropasqua ◽  
Vincenzo Fasanella ◽  
Mario Nubile ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Ocular Surface ◽  
Laser Scanning ◽  
Current Knowledge ◽  
Laser Scanning Confocal Microscopy ◽  
Cellular Level ◽  
Surgical Approaches ◽  
Imaging Method ◽  
Scanning Confocal Microscopy

AbstractOver the past decade, knowledge about the ocular surface in glaucoma has significantly increased through the use of in vivo laser scanning confocal microscopy (LSCM). This in vivo imaging method can show modifications at the cellular level induced by anti-glaucoma drugs on ocular surface structures and adnexa in the eye. High-quality images of the conjunctiva, cornea, limbus, meibomian glands, and lymphoid structures during therapy can be obtained. In addition, LSCM opened new fields of research on the patho-physiology of aqueous humor (AH) hydrodynamics in untreated, and in medically or surgically treated glaucomatous patients. In these conditions, an enhancement of the trans-scleral AH outflow contributed to clarification of the mechanism of action of different anti-glaucoma medications and surgical approaches. Finally, the use of LSCM represented a huge advance in evaluation of bleb functionality after filtration surgery, defining the hallmarks of AH filtration through the bleb-wall and distinguishing functional from nonfunctional blebs. Thus, signs seen with LSCM may anticipate clinical failure, guiding the clinician in planning the appropriate timing of the various steps in bleb management. In this review we summarize the current knowledge about in vivo LSCM of the ocular surface in glaucoma.

In Vivo Evaluation of Ocular Demodicosis Using Laser Scanning Confocal Microscopy

2011 ◽  
Vol 52 (1) ◽  
pp. 565 ◽  
Author(s):  
Takashi Kojima ◽  
Reiko Ishida ◽  
Enrique Adan Sato ◽  
Tetsuya Kawakita ◽  
Osama M. A. Ibrahim ◽  
...  
Keyword(s):  
Confocal Microscopy ◽  
Laser Scanning ◽  
Laser Scanning Confocal Microscopy ◽  
In Vivo Evaluation ◽  
Scanning Confocal Microscopy
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