Peak two-photon molecular brightness of fluorophores is a robust measure of quantum efficiency and photostability

2006 ◽  
Vol 23 (7) ◽  
pp. 1420 ◽  
Author(s):  
Vijay Iyer ◽  
Molly J. Rossow ◽  
M. Neal Waxham
Keyword(s):  
Quantum Efficiency ◽  
Two Photon ◽  
Molecular Brightness

scholarly journals Laser Induced Synthesis: Condensation of Acrylonitrile on Carvone

1988 ◽  
Vol 8 (2-4) ◽  
pp. 303-313
Author(s):  
R. Stringat ◽  
F. Lemaire ◽  
R. Fellous ◽  
A. M. Loiseau
Keyword(s):  
Quantum Yield ◽  
Absorption Band ◽  
Laser Irradiation ◽  
Quantum Efficiency ◽  
Photon Absorption ◽  
High Quantum Efficiency ◽  
Two Photon Absorption ◽  
Two Photon ◽  
Intermolecular Reaction ◽  
Absorption Channel

Laser irradiation of R(–) carvone in solution in acrylonitrile within the absorption band of its n−π* transition (≃350 nm) leads to the formation of cyclobutanic adducts, together with traces of camphocarvone.The carvone transformation is complete and appears with high quantum efficiency. The quantum yield for intermolecular reaction is intensity dependent (13–30%). This is ascribed to the fact that the reaction takes place via both a one and a two-photon absorption channel, the second being even more efficient.

Quantum efficiency for F-center production by two-photon absorption

1969 ◽  
Vol 7 (14) ◽  
pp. 1019-1022 ◽  
Author(s):  
M. Geller ◽  
T.A. De Temple ◽  
H.F. Taylor
Keyword(s):  
Quantum Efficiency ◽  
Photon Absorption ◽  
Two Photon Absorption ◽  
Two Photon ◽  
F Center

scholarly journals Yeast Ribosomal Stalk Heterogeneity In Vivo Shown by Two-Photon FCS and Molecular Brightness Analysis

2008 ◽  
Vol 94 (7) ◽  
pp. 2884-2890 ◽  
Author(s):  
Alberto García-Marcos ◽  
Susana A. Sánchez ◽  
Pilar Parada ◽  
John Eid ◽  
David M. Jameson ◽  
...  
Keyword(s):  
Two Photon ◽  
Brightness Analysis ◽  
Ribosomal Stalk ◽  
Molecular Brightness

Two-photon excitation microscopy in cellular biophysics

1995 ◽  
Vol 53 ◽  
pp. 62-63
Author(s):  
David W. Piston ◽  
Brian D. Bennett ◽  
Robert G. Summers
Keyword(s):  
Confocal Microscopy ◽  
Laser Beam ◽  
Duty Cycle ◽  
Excited Electronic State ◽  
Input Power ◽  
Two Photon ◽  
Simultaneous Absorption ◽  
Photon Excitation ◽  
Very High ◽  
Two Photon Excitation

Two-photon excitation microscopy (TPEM) provides attractive advantages over confocal microscopy for three-dimensionally resolved fluorescence imaging and photochemistry. Two-photon excitation arises from the simultaneous absorption of two photons in a single quantitized event whose probability is proportional to the square of the instantaneous intensity. For example, two red photons can cause the transition to an excited electronic state normally reached by absorption in the ultraviolet. In practice, two-photon excitation is made possible by the very high local instantaneous intensity provided by a combination of diffraction-limited focusing of a single laser beam in the microscope and the temporal concentration of 100 femtosecond pulses generated by a mode-locked laser. Resultant peak excitation intensities are 106 times greater than the CW intensities used in confocal microscopy, but the pulse duty cycle of 10-5 maintains the average input power on the order of 10 mW, only slightly greater than the power normally used in confocal microscopy.

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