scholarly journals Profiling of Differentially Expressed Genes in Roots of Robinia pseudoacacia during Nodule Development Using Suppressive Subtractive Hybridization

PLoS ONE ◽  
2013 ◽  
Vol 8 (6) ◽  
pp. e63930 ◽  
Author(s):  
Hongyan Chen ◽  
Minxia Chou ◽  
Xinye Wang ◽  
Sisi Liu ◽  
Feilong Zhang ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Robinia Pseudoacacia ◽  
Subtractive Hybridization ◽  
Differentially Expressed ◽  
Suppressive Subtractive Hybridization ◽  
Nodule Development

scholarly journals Screening of Differentially Expressed Microsporidia Genes from Nosema ceranae Infected Honey Bees by Suppression Subtractive Hybridization

Insects ◽  
2020 ◽  
Vol 11 (3) ◽  
pp. 199
Author(s):  
Zih-Ting Chang ◽  
Chong-Yu Ko ◽  
Ming-Ren Yen ◽  
Yue-Wen Chen ◽  
Yu-Shin Nai
Keyword(s):  
Gene Expression ◽  
Real Time ◽  
Differentially Expressed Genes ◽  
Honey Bees ◽  
Physiological Mechanism ◽  
Subtractive Hybridization ◽  
Infection Process ◽  
Nosema Ceranae ◽  
Differentially Expressed ◽  
Suppressive Subtractive Hybridization

The microsporidium Nosema ceranae is a high prevalent parasite of the European honey bee (Apis mellifera). This parasite is spreading across the world into its novel host. The developmental process, and some mechanisms of N. ceranae-infected honey bees, has been studied thoroughly; however, few studies have been carried out in the mechanism of gene expression in N. ceranae during the infection process. We therefore performed the suppressive subtractive hybridization (SSH) approach to investigate the candidate genes of N. ceranae during its infection process. All 96 clones of infected (forward) and non-infected (reverse) library were dipped onto the membrane for hybridization. A total of 112 differentially expressed sequence tags (ESTs) had been sequenced. For the host responses, 20% of ESTs (13 ESTs, 10 genes, and 1 non-coding RNA) from the forward library and 93.6% of ESTs (44 ESTs, 28 genes) from the reverse library were identified as differentially expressed genes (DEGs) of the hosts. A high percentage of DEGs involved in catalytic activity and metabolic processes revealed that the host gene expression change after N. ceranae infection might lead to an unbalance of physiological mechanism. Among the ESTs from the forward library, 75.4% ESTs (49 ESTs belonged to 24 genes) were identified as N. ceranae genes. Out of 24 N. ceranae genes, nine DEGs were subject to real-time quantitative reverse transcription PCR (real-time qRT-PCR) for validation. The results indicated that these genes were highly expressed during N. ceranae infection. Among nine N. ceranae genes, one N. ceranae gene (AAJ76_1600052943) showed the highest expression level after infection. These identified differentially expressed genes from this SSH could provide information about the pathological effects of N. ceranae. Validation of nine up-regulated N. ceranae genes reveal high potential for the detection of early nosemosis in the field and provide insight for further applications.

Transcriptomic approach to the study of osmoregulation in the European eelAnguilla anguilla

2007 ◽  
Vol 31 (3) ◽  
pp. 385-401 ◽  
Author(s):  
Svetlana Kalujnaia ◽  
Iain S. McWilliam ◽  
Vitalii A. Zaguinaiko ◽  
Anja L. Feilen ◽  
John Nicholson ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Sea Water ◽  
Subtractive Hybridization ◽  
Northern Blotting ◽  
Differentially Expressed ◽  
Suppressive Subtractive Hybridization ◽  
European Eel ◽  
Sargasso Sea ◽  
Functional Roles ◽  
Physiological Adaptations

In euryhaline teleosts, osmoregulation is a fundamental and dynamic process that is essential for the maintenance of ion and water balance, especially when fish migrate between fresh water (FW) and sea water (SW) environments. The European eel has proved to be an excellent model species to study the molecular and physiological adaptations associated with this osmoregulatory plasticity. The life cycle of the European eel includes two migratory periods, the second being the migration of FW eels back to the Sargasso Sea for reproduction. Various anatomical and physiological changes allow the successful transition to SW. The aim of this study was to use a microarray approach to screen the osmoregulatory tissues of the eel for changes in gene expression following acclimation to SW. Tissues were sampled from fish at selected intervals over a 5-mo period following FW/SW transfer, and RNA was isolated. Suppressive subtractive hybridization was used for enrichment of differentially expressed genes. Microarrays comprising 6,144 cDNAs from brain, gill, intestine, and kidney libraries were hybridized with appropriate targets and analyzed; 229 differentially expressed clones with unique sequences were identified. These clones represented the sequences for 95 known genes, with the remaining sequences (59%) being unknown. The results of the microarray analysis were validated by quantification of 28 differentially expressed genes by Northern blotting. A number of the differentially expressed genes were already known to be involved in osmoregulation, but the functional roles of many others, not normally associated with ion or water transport, remain to be characterized.

scholarly journals Profiling of BABA-induced differentially expressed genes of Zea mays using suppression subtractive hybridization

RSC Advances ◽  
2017 ◽  
Vol 7 (69) ◽  
pp. 43849-43865 ◽  
Author(s):  
Arun K. Shaw ◽  
Pardeep K. Bhardwaj ◽  
Supriya Ghosh ◽  
Ikbal Azahar ◽  
Sinchan Adhikari ◽  
...  
Keyword(s):  
Zea Mays ◽  
Differentially Expressed Genes ◽  
Suppression Subtractive Hybridization ◽  
Defense Mechanisms ◽  
Subtractive Hybridization ◽  
Differentially Expressed ◽  
Maize Leaves

This study aims to identify differentially expressed transcripts in BABA-primed maize leaves using suppression subtractive hybridization (SSH) strategy. Findings shed new light on the BABA potentiated defense mechanisms in plants.

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