Clubroot, caused by Plasmodiophora brassicae, is an important constraint on canola (Brassica napus) production in Canada. Rotations of clubroot-resistant (CR) canola cultivars in various sequences and planting intervals between canola with non-host crops and fallow periods were evaluated to determine their effects on clubroot severity and P. brassicae resting spore populations under field and micro-plot conditions. Under micro-plot conditions, the rotation sequences including CR canola, continuous fallow, and the non-host barley reduced gall weight by 63%–100% and clubroot severity by 34%–100% compared with continuous planting of susceptible canola. No visible clubroot symptoms developed following continuous fallow or the non-host crop. Under field conditions, clubroot severity was very high (78% disease index) in the continuous susceptible canola sequence. Most of the CR canola rotation sequences significantly reduced clubroot severity by 12%–23%, but continuous fallow, continuous barley, and alternating the CR canola cultivars ‘45H29’ or ‘73-47’ with ‘TC72429-10’ reduced clubroot severity by 32%–36%. A comparison of intervals between canola crops and four cropping sequences (continuous susceptible canola, alternating canola with barley or pea, a 2-yr non-host interval between canola crops, and a 3-yr non-host interval between canola crops) was conducted over 5 yr. A 2- or 3-yr non-host interval improved plant height, plant biomass, and seed yield, and reduced gall mass, P. brassicae propagules in the soil, and clubroot severity. A significant yield increase of more than 3600% was observed in a 3-yr non-host interval.
Endosphere microbiome comparison between symptomatic and asymptomatic roots of Brassica napus infected with Plasmodiophora brassicae
